- Volume 38 Issue 12
DOI QR Code
Corn Gluten Hydrolysate Affects the Time-Course of Metabolic Changes Through Appetite Control in High-Fat Diet-Induced Obese Rats
- Lee, Hyojung (Department of Nutritional Science and Food Management, Ewha Womans University) ;
- Lee, Hyo Jin (Department of Nutritional Science and Food Management, Ewha Womans University) ;
- Kim, Ji Yeon (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
- Kwon, Oran (Department of Nutritional Science and Food Management, Ewha Womans University)
- Received : 2015.04.23
- Accepted : 2015.09.18
- Published : 2015.12.31
This study first investigated the effects of corn gluten hydrolysate (CGH) (1.5 g/day) administration for 7 days on appetite-responsive genes in lean Sprague-Dawley (SD) rats. In a second set of experiments, the metabolic changes occurring at multiple time points over 8 weeks in response to CGH (35.33% wt/wt) were observed in high-fat (HF, 60% of energy as fat) diet-fed SD rats. In lean rats, the hypothalamus neuropeptide-Y and proopiomelanocortin mRNA levels of the CGH group were significantly changed in response to CGH administration. In the second part of the study, CGH treatment was found to reduce body weight and perirenal and epididymal fat weight. CGH also prevented an increase in food intake at 2 weeks and lowered plasma leptin and insulin levels in comparison with the HF group. This reduction in the plasma and hepatic lipid levels was followed by improved insulin resistance, and the beneficial metabolic effects of CGH were also partly related to increases in plasma adiponectin levels. The Homeostasis Model of Assessment - Insulin Resistance (HOMA-IR), an index of insulin resistance, was markedly improved in the HF-CGH group compared with the HF group at 6 weeks. According to the microarray results, adipose tissue mRNA expression related to G-protein coupled receptor protein signaling pathway and sensory perception was significantly improved after 8 weeks of CGH administration. In conclusion, the present findings suggest that dietary CGH may be effective for improving hyperglycemia, dyslipidemia and insulin resistance in diet-induced obese rats as well as appetite control in lean rats.
Grant : Research on New Functional Food Materials with Domestic Agricultural Products
Supported by : Rural Development Administration
- Kalra, S.P., Dube, M.G., Sahu, A., Phelps, C.P., and Kalra, P.S. (1991). Neuropeptide Y secretion increases in the paraventricular nucleus in association with increased appetite for food. Proc. Natl. Acad. Sci. USA 88, 10931-10935. https://doi.org/10.1073/pnas.88.23.10931
- Kim, S., Sohn, I., Ahn, J.I., Lee, K.H., and Lee, Y.S. (2004). Hepatic gene expression profiles in a long-term high-fat diet-induced obesity mouse model. Gene 340, 99-109. https://doi.org/10.1016/j.gene.2004.06.015
- Kim, J., Park, J., Hong, S., and Kim, M.K. (2009). Effect of corn gluten and its hydrolysate consumptions on weight reduction in rats fed a high-fat diet. Nutr. Res. Pract. 3, 200-207. https://doi.org/10.4162/nrp.2009.3.3.200
- Kleemann, R., van Erk, M., Verschuren, L., van den Hoek, A.M., Koek, M., Wielinga, P.Y., Jie, A., Pellis, L., Bobeldijk-Pastorova, I., Kelder, T., et al. (2010). Time-resolved and tissue-specific systems analysis of the pathogenesis of insulin resistance. PLoS One 5, e8817. https://doi.org/10.1371/journal.pone.0008817
- Kopelman, P.G. (2000). Obesity as a medical problem. Nature 404, 635-643. https://doi.org/10.1038/35007508
- Layman, D.K. (2003). The role of leucine in weight loss diets and glucose homeostasis. J. Nutr. 133, 261S-267S. https://doi.org/10.1093/jn/133.1.261S
- Layman, D.K., and Walker, D.A. (2006). Potential importance of leucine in treatment of obesity and the metabolic syndrome. J. Nutr. 136, 319S-323S. https://doi.org/10.1093/jn/136.1.319S
- Maffei, M., Halaas, J., Ravussin, E., Pratley, R.E., Lee, G.H., Zhang, Y., Fei, H., Kim, S., Lallone, R., Ranganathan, S., et al. (1995). Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nat. Med. 1, 1155-1161. https://doi.org/10.1038/nm1195-1155
- Mercer, A.J., Hentges, S.T., Meshul, C.K., and Low, M.J. (2013). Unraveling the central proopiomelanocortin neural circuits. Front Neurosci. 7, 19.
- Mun, J.M., Ok, H.M., and Kwon, O. (2014). Corn gluten hydrolysate and capsaicin have complimentary actions on body weight reduction and lipid-related genes in diet-induced obese rats. Nutr. Res. 34, 458-465. https://doi.org/10.1016/j.nutres.2014.04.009
- Negroni, J., Meunier, N., Monnerie, R., Salesse, R., Baly, C., Caillol, M., and Congar, P. (2012). Neuropeptide y enhances olfactory mucosa responses to odorant in hungry rats. PLoS One 7, e45266. https://doi.org/10.1371/journal.pone.0045266
- Ng, S.-F., Lin, R.C., Maloney, C.A., Youngson, N.A., Owens, J.A., and Morris, M.J. (2014). Paternal high-fat diet consumption induces common changes in the transcriptomes of retroperitoneal adipose and pancreatic islet tissues in female rat offspring. FASEB J. 28, 1830-1841. https://doi.org/10.1096/fj.13-244046
- Beck, B., Jhanwar-Uniyal, M., Burlet, A., Chapleur-Chateau, M., Leibowitz, S.F., and Burlet, C. (1990). Rapid and localized alterations of neuropeptide Y in discrete hypothalamic nuclei with feeding status. Brain Res. 528, 245-249. https://doi.org/10.1016/0006-8993(90)91664-3
- Bligh, E.G., and Dyer, W.J. (1959). A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911-917. https://doi.org/10.1139/o59-099
- Bong, H.Y., Kim, J.Y., Jeong, H.I., Moon, M.S., Kim, J., and Kwon, O. (2010). Effects of corn gluten hydrolyzates, branched chain amino acids, and leucine on body weight reduction in obese rats induced by a high fat diet. Nutr. Res. Pract 4, 106-113. https://doi.org/10.4162/nrp.2010.4.2.106
- Calder, P.C., Ahluwalia, N., Brouns, F., Buetler, T., Clement, K., Cunningham, K., Esposito, K., Jonsson, L.S., Kolb, H., Lansink, M., et al. (2011). Dietary factors and low-grade inflammation in relation to overweight and obesity. Br. J. Nutr. 106, S5-78. https://doi.org/10.1017/S0007114511005460
- Caudill, S.P., Cooper, G.R., Smith, S.J., and Myers, G.L. (1998). Assessment of current National Cholesterol Education Program guidelines for total cholesterol triglyceride, HDL-cholesterol, and LDL-cholesterol measurements. Clin Chem 44, 1650-1658.
- Clark, M.J., and Slavin, J.L. (2013). The effect of fiber on satiety and food intake: a systematic review. J. Am. Coll. Nutr. 32, 200-211. https://doi.org/10.1080/07315724.2013.791194
- Das, U.N. (2010). Obesity: genes, brain, gut, and environment. Nutrition 26, 459-473. https://doi.org/10.1016/j.nut.2009.09.020
- Davis, J., Steinle, J., Higginbotham, D., Oitker, J., Peterson, R., and Banz, W. (2005). Soy protein influences insulin sensitivity and cardiovascular risk in male lean SHHF rats. Horm. Metab. Res. 37, 309-315. https://doi.org/10.1055/s-2005-861475
- Do, G.M., Oh, H.Y., Kwon, E.Y., Cho, Y.Y., Shin, S.K., Park, H.J., Jeon, S.M., Kim, E., Hur, C.G., Park, T.S., et al. (2011). Long-term adaptation of global transcription and metabolism in the liver of high-fat diet-fed C57BL/6J mice. Mol. Nutr. Food Res. 55, S173-185. https://doi.org/10.1002/mnfr.201100064
- Fraulob, J.C., Ogg-Diamantino, R., Fernandes-Santos, C., Aguila, M.B., and Mandarim-de-Lacerda, C.A. (2010). A mouse model of metabolic syndrome: insulin resistance, fatty liver and nonalcoholic fatty pancreas disease (NAFPD) in C57BL/6 mice fed a high fat diet. J. Clin. Biochem. Nutr. 46, 212-223. https://doi.org/10.3164/jcbn.09-83
- Gehlert, D.R., Chronwall, B.M., Schafer, M.P., and O'Donohue, T.L. (1987). Localization of neuropeptide Y messenger ribonucleic acid in rat and mouse brain by in situ hybridization. Synapse 1, 25-31. https://doi.org/10.1002/syn.890010106
- Huang, D.W., Sherman, B.T., and Lempicki, R.A. (2008). Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat. Protoc. 4, 44-57. https://doi.org/10.1038/nprot.2008.211
- Jequier, E. (2002). Leptin signaling, adiposity, and energy balance. Ann. N Y Acad. Sci. 967, 379-388.
- Joo, J.I., Kim, D.H., Choi, J.-W., and Yun, J.W. (2010). Proteomic analysis for antiobesity potential of capsaicin on white adipose tissue in rats fed with a high fat diet. J. Proteome Res. 9, 2977-2987. https://doi.org/10.1021/pr901175w
- Adam, C., Williams, P., Garden, K., Thomson, L., and Ross, A. (2014). Dose-dependent effects of a soluble dietary fibre (pectin) on food intake, adiposity, gut hypertrophy and gut satiety hormone secretion in rats. PLoS One 10, e0115438-e0115438.
- Aoyama, T., Fukui, K., Takamatsu, K., Hashimoto, Y., and Yamamoto, T. (2000). Soy protein isolate and its hydrolysate reduce body fat of dietary obese rats and genetically obese mice (yellow KK). Nutrition 16, 349-354. https://doi.org/10.1016/S0899-9007(00)00230-6
- Ashburner, M., Ball, C.A., Blake, J.A., Botstein, D., Butler, H., Cherry, J.M., Davis, A.P., Dolinski, K., Dwight, S.S., Eppig, J.T., et al. (2000). Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat. Genet. 25, 25-29. https://doi.org/10.1038/75556
- Panteghini, M., Pagani, F., and Bonora, R. (1993). Clinical and analytical evaluation of a continuous enzymatic method for measuring pancreatic lipase activity. Clin. Chem. 39, 304-308.
- Radonjic, M., de Haan, J.R., van Erk, M.J., van Dijk, K.W., van den Berg, S.A., de Groot, P.J., Muller, M., and van Ommen, B. (2009). Genome-wide mRNA expression analysis of hepatic adaptation to high-fat diets reveals switch from an inflammatory to steatotic transcriptional program. PLoS One 4, e6646. https://doi.org/10.1371/journal.pone.0006646
- Schneeberger, M., Gomis, R., and Claret, M. (2014). Hypothalamic and brainstem neuronal circuits controlling homeostatic energy balance. J. Endocrinol. 220, T25-46. https://doi.org/10.1530/JOE-13-0398
- Slavin, J. (2013). Fiber and prebiotics: mechanisms and health benefits. Nutrients 5, 1417-1435. https://doi.org/10.3390/nu5041417
- Slavin, J., and Green, H. (2007). Dietary fibre and satiety. Nutr. Bull. 32, 32-42. https://doi.org/10.1111/j.1467-3010.2007.00603.x
- Wanders, A.J., van den Borne, J.J., de Graaf, C., Hulshof, T., Jonathan, M.C., Kristensen, M., Mars, M., Schols, H.A., and Feskens, E.J. (2011). Effects of dietary fibre on subjective appetite, energy intake and body weight: a systematic review of randomized controlled trials. Obes. Rev. 12, 724-739.