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세포의 신호전달 과정에서 NO 항상성에 관한 융복합 연구

Convergence Studies of NO Homeostasis in Cellular Signalling

  • 오희균 (전남대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 도은영 (광주여자대학교 긴호학과) ;
  • 박해령 (광주여자대학교 교양교직과정부)
  • Oh, Hee-Kyun (Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University) ;
  • Do, Eun-Young (Department of Nursing, Kwangju Women's University) ;
  • Park, Hae-Ryoung (Division of Liberal Arts & Teacher Training, Kwangju Women's University)
  • 투고 : 2015.10.24
  • 심사 : 2015.12.20
  • 발행 : 2015.12.28

초록

목향(Saussurea lappa)은 다양한 생리활성을 나타내는 성분들은 알려져 있으나 세포내에서 신호전달 체계에 미치는 영향에 대해서 자세히 알려진 바가 없다. 본 연구에서 목향의 에탄올 추출물이 Raw 264.7세포의 NO(nitric oxide) 항상성에 대한 논의를 하고자 한다. LPS(lipolysaccharide)로 유도한 후, NO(nitric oxide) 생성억제력과 IL-$1{\beta}$(interleukin-$1{\beta}$), TNF-${\alpha}$(tumour necrosis factor-${\alpha}$), iNOS와 COX-2를 전사수준(transcriptional level)에서 발현양상을 살펴보고자 한다. 목향의 에탄올 추출물은 RAW264.7 세포에서 LPS를 전처리한 후 유도되는 iNOS와 COX-2가 전사 수준에서 발현 억제와 pro-inflammatory cytokine인 TNF-${\alpha}$와 IL-$1{\beta}$의 발현을 효과적으로 저해함을 보였다. 본 연구 결과로 목향의 에탄올 추출물이 대식세포를 매개로 한 염증반응의 작용기전 연구에 있어서 NO(nitric oxide) 항상성에 영향이 있음을 융복합적 측면에서 고찰하였다. 향후 염증성 질환의 예방과 치료에 중요한 기초 자료로 제공하고 자 한다.

키워드

목향;신호전달;융복합;항상성

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