Convergence Studies of NO Homeostasis in Cellular Signalling

세포의 신호전달 과정에서 NO 항상성에 관한 융복합 연구

  • Oh, Hee-Kyun (Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University) ;
  • Do, Eun-Young (Department of Nursing, Kwangju Women's University) ;
  • Park, Hae-Ryoung (Division of Liberal Arts & Teacher Training, Kwangju Women's University)
  • 오희균 (전남대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 도은영 (광주여자대학교 긴호학과) ;
  • 박해령 (광주여자대학교 교양교직과정부)
  • Received : 2015.10.24
  • Accepted : 2015.12.20
  • Published : 2015.12.28


Saussurea lappa is known for a variety of physiological activities as a component but has not known to show the effect of the cellular signaling pathway. We investigated the anti-inflammatory effects by Saussurea lappa ethanol extract on the LPS(lipopolysaccharide) induced nitric oxide (NO) production by RAW 264.7 cell line. It shows the expressions of iNOS and COX-2 at the transcriptional level (RT-PCR). The Saussurea lappa ethanol extract showed transcriptional expression levels of pro-inflammatory cytokine TNF- and IL-$1{\beta}$ induced by LPS(lipolysaccharide) in RAW264.7 cell line. Saussurea lappa ethanol extract reduced the LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the RNA levels in a concentration-dependent manner. The finding that ethanol extract of Saussurea lappa has an influence on NO (nitric oxide) homeostasis in the study of the action mechanism on the macrophage-mediated inflammatory reaction was considered in terms of convergence. And it is to provide an important basis for the prevention and treatment of inflammatory diseases in the future.


Cellular Signalling;COX-2(cyclooxygenase-2);Convergence;Homeostasis;NO(nitric oxide);Saussurea lappa


  1. P. Batchelor, The changing epidemiology of oral diseases in the elderly, their growing importance for care and how they can be managed. Age Ageing, Vol. 44, No. 6, pp. 1064-1070, 2015.
  2. D. J. Zuluaga, J. Ferreira, J. A. Montoya, T. Willumsen, Oral health in institutionalised elderly people in Oslo, Norway and its relationship with dependence and cognitive impairment. Gerodontology, Vol. 29, No. 2, pp. 420-426, 2012.
  3. K. Sanjeev, S. Naganandini, R. Vasuda, N. Sachin, R. Rekha, M. S. Madhuniranjanswamy, Oral Hygiene Status of Institutionalised Dependent Elderly in India - a Cross-Sectional Survey. Can Geriatr J., Vol. 18, No. 2, pp. 51-56, 2015.
  4. F. Cindy, Iron Deficiency Anemia: A Guide to Oral Iron Supplements. Clinical Correlation, 2015.
  5. M. Harold, C. L. Marc, Oral Microbial Ecology and the Role of Salivary Immunoglobulin A. Microbiol. Mol. Biol. Rev. Vol. 62 No. 1 pp. 71-109, 1998.
  6. W. C. Nance, S. E. Dowd, D. Samarian, J. Chludzinski, J. Delli, J. Battista, A. H. Rickard, A high-throughput microfluidic dental plaque biofilm system to visualize and quantify the effect of antimicrobials. Antimicrob Chemother, Vol. 68, No. 11, pp. 2550-2560, J 2013.
  7. D. M. Philip, Dental plaque as a biofilm and a microbial community-implications for health and disease. BMC Oral Health, Vol. 6(Suppl 1):S14, 2006.
  8. J. Y. Choi, E. H. Choi, H. W. Jung, J. S. Oh, W. H. Lee, J. G. Lee, J. K. Son, Y. Kim, S. H. Lee, Melanogenesis inhibitory compounds from Saussureae Radix. Arch Pharm Res. Vol. 31, No. 3, pp. 294-299, 2008.
  9. N. H. Jung, J. Y. Ha, K. R. Min, F. Shibata, H. Nakagawa, S. S. Kang, I. M. Chang, Y. S. Kim, Reynosis from Saussurea lappa as inhibitor on CINC-1 induction in LPS-stimulated NRK-52E cells. Planta Med, Vol. 64, pp. 454-456, 1998.
  10. Y. J. Jeon, H. S. Lee, S. Y. Yeon, J. H. Ko, K. M. An, S. W. Yu, J. H. Kang, B. Y. Hwang, T. Y. Kin. Inhibitory effect of dehydrocostuslactone isolated from Sussureae Radix on CDK2 activity. Kor J Pharmacogn 36: 97-101.2005.
  11. J. L. Ebersole, M. A. Taubmam, The protective nature of hostresponses in periodontal diseases. Periodontol 2000, Vol. 5, pp. 112-141, 1994.
  12. K. S. Kornman, R. C. Page, M. S. Tonetti, The host response tothe microbial challenge in periodontitis: assembling the play-ers. Periodontol 2000, Vol. 14, pp. 33-53, 1997.
  13. H. R. Park, S. J. Hong, Research on Natural Medicine for Wellness and Oral Health, The Journal of Digital Policy & Management, Vol. 13, No. 5, pp. 357-1363, 2015.
  14. T. Kirikae, T. Nitta, F. Kirikae, T. Suda, S. Kusumoto, N. Qureshi, M. Nakano, Lipopolysaccharides (LPS) of oral black pigmented bacteria induce tumor necrosis factor production by LPS-refractory C3H/HeJ macrophages in a way different fromthat of Salmonella LPS. Infect Immun, Vol. 67, pp. 17-36, 1999.
  15. K. Seibert, Y. Zhang, K. Leahy, S. Hauser, J. Masferrer, W. Perkins, L. Lee, P. Ksakson, Pharmacological andbiochemical demonstration of the role of cyclooxygenase 2 ininflammation and pain. Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, pp. 12013-12017, 1994.
  16. M. Feldmann, F. M. Brennan, R. N. Maini, Role of cytokines in rheumatoid arthritis. Annual Review of Immunology, Vol. 14, pp. 397-440, 1996.
  17. Y. C. Hseu, F. Y. Wu, J. J. Wu, J. Y. Chen, W. H. Chang, F. J. Lu, Y. C. Lai, H. L. Yang, Anti-inflammatory potential of Antrodia Camphorata through inhibition of iNOS, COX-2 and cytokines via the NF-kappaB pathway. Int Immunopharmacol, Vol. 5, pp. 1914-1925, 2005.
  18. B. Rudi, M. Alexander, Induction of nitric oxide synthase by cytokines in vascular smooth muscle cells, and children. FEBS Letters, Vol. 275, No. 1-2, pp. 87-90, 1990.
  19. M. Takeshi, N. Toshio, H. Keiichi, S. Hiromichi, K. Ryuichi, S. Takao, Cyclosporin A Inhibits Nitric Oxide Synthase Induction in Vascular Smooth Muscle Cells. Hypertension, Vol. 25, pp. 764-768, 1995.
  20. M. Cetkovic-Cvrlje, D. L. Eizirik, TNF and IFN ${\gamma}$ potentiate the deleterious effects of IL-$1{\beta}$ on mouse pancreatic islets mainly viageneration of nitric oxide. Cytokine, Vol. 6, pp. 399-406, 1994.