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Convergence Studies of NO Homeostasis in Cellular Signalling

세포의 신호전달 과정에서 NO 항상성에 관한 융복합 연구

  • Oh, Hee-Kyun (Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University) ;
  • Do, Eun-Young (Department of Nursing, Kwangju Women's University) ;
  • Park, Hae-Ryoung (Division of Liberal Arts & Teacher Training, Kwangju Women's University)
  • 오희균 (전남대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 도은영 (광주여자대학교 긴호학과) ;
  • 박해령 (광주여자대학교 교양교직과정부)
  • Received : 2015.10.24
  • Accepted : 2015.12.20
  • Published : 2015.12.28

Abstract

Saussurea lappa is known for a variety of physiological activities as a component but has not known to show the effect of the cellular signaling pathway. We investigated the anti-inflammatory effects by Saussurea lappa ethanol extract on the LPS(lipopolysaccharide) induced nitric oxide (NO) production by RAW 264.7 cell line. It shows the expressions of iNOS and COX-2 at the transcriptional level (RT-PCR). The Saussurea lappa ethanol extract showed transcriptional expression levels of pro-inflammatory cytokine TNF- and IL-$1{\beta}$ induced by LPS(lipolysaccharide) in RAW264.7 cell line. Saussurea lappa ethanol extract reduced the LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the RNA levels in a concentration-dependent manner. The finding that ethanol extract of Saussurea lappa has an influence on NO (nitric oxide) homeostasis in the study of the action mechanism on the macrophage-mediated inflammatory reaction was considered in terms of convergence. And it is to provide an important basis for the prevention and treatment of inflammatory diseases in the future.

Keywords

Cellular Signalling;COX-2(cyclooxygenase-2);Convergence;Homeostasis;NO(nitric oxide);Saussurea lappa

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