Preparation of Yeast Hydrolysate Enriched in Cyclo-His-Pro (CHP) by Enzymatic Hydrolysis and Evaluation of Its Functionality

  • Lee, Hyun Jung (Department of Public Health Science, Graduate School, Korea University) ;
  • Son, Heung Soo (Department of Food Science and Biotechnology, Shin Ansan University) ;
  • Park, Chung (LINC Project Group, Daejeon University) ;
  • Suh, Hyung Joo (Department of Public Health Science, Graduate School, Korea University)
  • Received : 2015.08.25
  • Accepted : 2015.11.25
  • Published : 2015.12.31


In this study, we attempted to enrich cyclo-His-Pro (CHP) using enzymatic hydrolysis of yeast and to evaluate the functionality of yeast hydrolysate (YH)-enriched CHP. Flavourzyme offered a better performance in enhancing CHP content than other proteases. The CHP enrichment conditions were optimized as follows: addition of 1% Flavourzyme, 48-h incubation at 60oC, and pH 6.0. The CHP content significantly increased by 20-fold after ultra-filtration (UF). Maximal CHP translation was obtained after heating for 8 h at 50oC and pH 7.0. YH showed poor foaming capacity between pH 3.0 to 9.0. The emulsifying activities of YHs were slightly higher at near acidic pH. Increase in heating temperature and time resulted in decreased CHP content. The results indicate that YH is more heat stable after UF. Therefore, the CHP in YH after UF can be used as a food additive with physiological CHP activity and high heat stability.


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