Mitigating Effect of Resveratrol on the Structural Changes of Mice Liver and Kidney Induced by Cadmium; A Stereological Study

  • Rafati, Ali (Histomorphometry and Stereology Research Center) ;
  • Hoseini, Leila (Department of Traditional Medicine and History of Medicine, Shiraz University of Medical Sciences) ;
  • Babai, Ali (Histomorphometry and Stereology Research Center) ;
  • Noorafshan, Ali (Histomorphometry and Stereology Research Center) ;
  • Haghbin, Hossein (Histomorphometry and Stereology Research Center) ;
  • Karbalay-Doust, Saied (Histomorphometry and Stereology Research Center)
  • Received : 2015.06.08
  • Accepted : 2015.11.03
  • Published : 2015.12.31


Exposure to cadmium (Cd) has harmful effects on the liver and kidney. Resveratrol (RES) is an herbal substance that functions as a protective mediator. This study aimed to investigate the effects of RES on the histology of liver and kidney in Cd-exposed mice. Male mice were divided into 4 groups daily receiving normal saline (1 mL normal saline/d), Cd (1 mg/kg/d), RES (20 mg/kg/d), and Cd plus RES, respectively. After 4 weeks, the liver and kidney components were evaluated using stereological methods. The total volume and number of hepatocytes, and volume of fibrous tissue were respectively increased by 34%, 58%, and a 3-fold in the Cd-exposed mice in comparison to the control animals (P<0.03). On the other hand, the volume of the main vasculature (sinusoids and central veins) was decreased by 36% in the Cd group compared to the control mice (P<0.03). Considering the kidney, the results showed a 3-fold increase in the total glomeruli volume and a 7-fold increase in fibrous tissue in the Cd-treated group compared to the control mice (P<0.03). After Cd treatment, a 32% reduction was observed in the volume and length of the proximal and distal convoluted tubules. RES-treatment alone did not induce any structural changes. In comparison to the Cd group, an increase in the normal components of the liver and kidney and a decrease in the formation of the fibrous and degenerated tissues were observed in the Cd+RES-treated mice (P<0.03).


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