DOI QR코드

DOI QR Code

Mitigating Effect of Resveratrol on the Structural Changes of Mice Liver and Kidney Induced by Cadmium; A Stereological Study

  • Rafati, Ali (Histomorphometry and Stereology Research Center) ;
  • Hoseini, Leila (Department of Traditional Medicine and History of Medicine, Shiraz University of Medical Sciences) ;
  • Babai, Ali (Histomorphometry and Stereology Research Center) ;
  • Noorafshan, Ali (Histomorphometry and Stereology Research Center) ;
  • Haghbin, Hossein (Histomorphometry and Stereology Research Center) ;
  • Karbalay-Doust, Saied (Histomorphometry and Stereology Research Center)
  • Received : 2015.06.08
  • Accepted : 2015.11.03
  • Published : 2015.12.31

Abstract

Exposure to cadmium (Cd) has harmful effects on the liver and kidney. Resveratrol (RES) is an herbal substance that functions as a protective mediator. This study aimed to investigate the effects of RES on the histology of liver and kidney in Cd-exposed mice. Male mice were divided into 4 groups daily receiving normal saline (1 mL normal saline/d), Cd (1 mg/kg/d), RES (20 mg/kg/d), and Cd plus RES, respectively. After 4 weeks, the liver and kidney components were evaluated using stereological methods. The total volume and number of hepatocytes, and volume of fibrous tissue were respectively increased by 34%, 58%, and a 3-fold in the Cd-exposed mice in comparison to the control animals (P<0.03). On the other hand, the volume of the main vasculature (sinusoids and central veins) was decreased by 36% in the Cd group compared to the control mice (P<0.03). Considering the kidney, the results showed a 3-fold increase in the total glomeruli volume and a 7-fold increase in fibrous tissue in the Cd-treated group compared to the control mice (P<0.03). After Cd treatment, a 32% reduction was observed in the volume and length of the proximal and distal convoluted tubules. RES-treatment alone did not induce any structural changes. In comparison to the Cd group, an increase in the normal components of the liver and kidney and a decrease in the formation of the fibrous and degenerated tissues were observed in the Cd+RES-treated mice (P<0.03).

References

  1. Govil N, Chaudhary S, Waseem M, Parvez S. 2012. Postnuclear supernatant: an in vitro model for assessing cadmiuminduced neurotoxicity. Biol Trace Elem Res 146: 402-409. https://doi.org/10.1007/s12011-011-9263-y
  2. Romero A, Ramos E, de Los Rios C, Egea J, Del Pino J, Reiter RJ. 2014. A review of metal-catalyzed molecular damage: protection by melatonin. J Pineal Res 56: 343-370. https://doi.org/10.1111/jpi.12132
  3. Shimada H, Hashiguchi T, Yasutake A, Waalkes MP, Imamura Y. 2012. Sexual dimorphism of cadmium-induced toxicity in rats: involvement of sex hormones. Arch Toxicol 86: 1475-1480. https://doi.org/10.1007/s00204-012-0844-0
  4. Rani A, Kumar A, Lal A, Pant M. 2014. Cellular mechanisms of cadmium-induced toxicity: a review. Int J Environ Health Res 24: 378-399. https://doi.org/10.1080/09603123.2013.835032
  5. Kara H, Cevik A, Konar V, Dayangac A, Servi K. 2008. Effects of selenium with vitamin E and melatonin on cadmiuminduced oxidative damage in rat liver and kidneys. Biol Trace Elem Res 125: 236-244. https://doi.org/10.1007/s12011-008-8168-x
  6. Gong P, Chen FX, Wang L, Wang J, Jin S, Ma YM. 2014. Protective effects of blueberries (Vaccinium corymbosum L.) extract against cadmium-induced hepatotoxicity in mice. Environ Toxicol Pharmacol 37: 1015-1027. https://doi.org/10.1016/j.etap.2014.03.017
  7. Al-Attar AM. 2011. Vitamin E attenuates liver injury induced by exposure to lead, mercury, cadmium and copper in albino mice. Saudi J Biol Sci 18: 395-401. https://doi.org/10.1016/j.sjbs.2011.07.004
  8. Hyder O, Chung M, Cosgrove D, Herman JM, Li Z, Firoozmand A, Gurakar A, Koteish A, Pawlik TM. 2013. Cadmium exposure and liver disease among US adults. J Gastrointest Surg 17: 1265-1273. https://doi.org/10.1007/s11605-013-2210-9
  9. Renugadevi J, Prabu SM. 2010. Quercetin protects against oxidative stress-related renal dysfunction by cadmium in rats. Exp Toxicol Pathol 62: 471-481. https://doi.org/10.1016/j.etp.2009.06.006
  10. Waalkes MP. 2000. Cadmium carcinogenesis in review. J Inorg Biochem 79: 241-244. https://doi.org/10.1016/S0162-0134(00)00009-X
  11. Lakshmi GD, Kumar PR, Bharavi K, Annapurna P, Rajendar B, Patel PT, Kumar CS, Rao GS. 2012. Protective effect of Tribulus terrestris Linn on liver and kidney in cadmium intoxicated rats. Indian J Exp Biol 50: 141-146.
  12. Tripathi S, Srivastav AK. 2011. Cytoarchitectural alterations in kidney of Wistar rat after oral exposure to cadmium chloride. Tissue Cell 43: 131-136. https://doi.org/10.1016/j.tice.2011.01.001
  13. Xiao J, So KF, Liong EC, Tipoe GL. 2013. Recent advances in the herbal treatment of non-alcoholic fatty liver disease. J Tradit Complement Med 3: 88-94. https://doi.org/10.4103/2225-4110.110411
  14. Powell RD, Swet JH, Kennedy KL, Huynh TT, McKillop IH, Evans SL. 2014. Resveratrol attenuates hypoxic injury in a primary hepatocyte model of hemorrhagic shock and resuscitation. J Trauma Acute Care Surg 76: 409-417. https://doi.org/10.1097/TA.0000000000000096
  15. Hussein MA. 2013. Prophylactic effect of resveratrol against ethinylestradiol-induced liver cholestasis. J Med Food 16: 246-254. https://doi.org/10.1089/jmf.2012.0183
  16. Palsamy P, Sivakumar S, Subramanian S. 2010. Resveratrol attenuates hyperglycemia-mediated oxidative stress, proinflammatory cytokines and protects hepatocytes ultrastructure in streptozotocin-nicotinamide-induced experimental diabetic rats. Chem Biol Interact 186: 200-210. https://doi.org/10.1016/j.cbi.2010.03.028
  17. Paul A, Das J, Das S, Samadder A, Khuda-Bukhsh AR. 2013. Poly (lactide-co-glycolide) nano-encapsulation of chelidonine, an active bioingredient of greater celandine (Chelidonium majus), enhances its ameliorative potential against cadmium induced oxidative stress and hepatic injury in mice. Environ Toxicol Pharmacol 36: 937-947. https://doi.org/10.1016/j.etap.2013.08.008
  18. Lin HC, Chen YF, Hsu WH, Yang CW, Kao CH, Tsai TF. 2012. Resveratrol helps recovery from fatty liver and protects against hepatocellular carcinoma induced by hepatitis B virus X protein in a mouse model. Cancer Prev Res (Phila) 5: 952-962. https://doi.org/10.1158/1940-6207.CAPR-12-0001
  19. Karbalay-Doust S, Noorafshan A. 2009. Stereological study of the effects of nandrolone decanoate on the mouse liver. Micron 40: 471-475. https://doi.org/10.1016/j.micron.2008.12.006
  20. Marcos R, Monteiro RA, Rocha E. 2012. The use of designbased stereology to evaluate volumes and numbers in the liver: a review with practical guidelines. J Anat 220: 303-317. https://doi.org/10.1111/j.1469-7580.2012.01475.x
  21. Dorph-Petersen KA, Nyengaard JR, Gundersen HJ. 2001. Tissue shrinkage and unbiased stereological estimation of particle number and size. J Microsc 204: 232-246. https://doi.org/10.1046/j.1365-2818.2001.00958.x
  22. von Bartheld CS. 2012. Distribution of particles in the Z-axis of tissue sections: relevance for counting methods. Neuroquantology 10: 66-75.
  23. Cupertino MC, Costa KL, Santos DC, Novaes RD, Condessa SS, Neves AC, Oliveira JA, Matta SL. 2013. Long-lasting morphofunctional remodelling of liver parenchyma and stroma after a single exposure to low and moderate doses of cadmium in rats. Int J Exp Pathol 94: 343-351. https://doi.org/10.1111/iep.12046
  24. Marcano L, Faria Cde R, Carruyo I, Montiel X. 2006. Cadmium cytotoxicity in mice hepatocytes and implications on tropical environments. Rev Biol Trop 54: 257-263.
  25. Siddiqi NJ, Zargar S. 2014. Effect of quercetin on cadmium fluoride-induced alterations in hydroxyproline/collagen content in mice liver. Connect Tissue Res 55: 234-238. https://doi.org/10.3109/03008207.2014.900551
  26. de Almeida TM, Leitao RC, Andrade JD, Becak W, Carrilho FJ, Sonohara S. 2004. Detection of micronuclei formation and nuclear anomalies in regenerative nodules of human cirrhotic livers and relationship to hepatocellular carcinoma. Cancer Genet Cytogenet 150: 16-21. https://doi.org/10.1016/j.cancergencyto.2003.08.001
  27. Kim BM, Lee SY, Jeong IH. 2013. Influence of squid liver powder on accumulation of cadmium in serum, kidney and liver of mice. Prev Nutr Food Sci 18: 1-10. https://doi.org/10.3746/pnf.2013.18.1.001
  28. Wu X, Li C, Xing G, Qi X, Ren J. 2013. Resveratrol downregulates Cyp2e1 and attenuates chemically induced hepatocarcinogenesis in SD rats. J Toxicol Pathol 26: 385-392. https://doi.org/10.1293/tox.2013-0020
  29. Barrouillet MP, Potier M, Cambar J. 1999. Cadmium nephrotoxicity assessed in isolated rat glomeruli and cultured mesangial cells: evidence for contraction of glomerular cells. Exp Nephrol 7: 251-258. https://doi.org/10.1159/000020610
  30. Chargui A, Zekri S, Jacquillet G, Rubera I, Ilie M, Belaid A, Duranton C, Tauc M, Hofman P, Poujeol P, El May MV, Mograbi B. 2011. Cadmium-induced autophagy in rat kidney: an early biomarker of subtoxic exposure. Toxicol Sci 121: 31-42. https://doi.org/10.1093/toxsci/kfr031
  31. Prozialeck WC, Edwards JR. 2012. Mechanisms of cadmiuminduced proximal tubule injury: new insights with implications for biomonitoring and therapeutic interventions. J Pharmacol Exp Ther 343: 2-12. https://doi.org/10.1124/jpet.110.166769
  32. Eybl V, Kotyzova D, Koutensky J. 2006. Comparative study of natural antioxidants-curcumin, resveratrol and melatonin-in cadmium-induced oxidative damage in mice. Toxicology 225: 150-156. https://doi.org/10.1016/j.tox.2006.05.011
  33. Morales AI, Rodriguez-Barbero A, Vicente-Sanchez C, Mayoral P, Lopez-Novoa JM, Perez-Barriocanal F. 2006. Resveratrol inhibits gentamicin-induced mesangial cell contraction. Life Sci 78: 2373-2377. https://doi.org/10.1016/j.lfs.2005.09.045
  34. Chander V, Chopra K. 2006. Protective effect of nitric oxide pathway in resveratrol renal ischemia-reperfusion injury in rats. Arch Med Res 37: 19-26. https://doi.org/10.1016/j.arcmed.2005.05.018
  35. Chander V, Chopra K. 2006. Protective effect of resveratrol, a polyphenolic phytoalexin on glycerol-induced acute renal failure in rat kidney. Ren Fail 28: 161-169. https://doi.org/10.1080/08860220500531112
  36. Do Amaral CL, Francescato HD, Coimbra TM, Costa RS, Darin JD, Antunes LM, Bianchi Mde L. 2008. Resveratrol attenuates cisplatin-induced nephrotoxicity in rats. Arch Toxicol 82: 363-370. https://doi.org/10.1007/s00204-007-0262-x
  37. Tikoo K, Singh K, Kabra D, Sharma V, Gaikwad A. 2008. Change in histone H3 phosphorylation, MAP kinase p38, SIR 2 and p53 expression by resveratrol in preventing streptozotocin induced type I diabetic nephropathy. Free Radic Res 42: 397-404. https://doi.org/10.1080/10715760801998646

Cited by

  1. Endoplasmic reticulum stress eIF2α–ATF4 pathway-mediated cyclooxygenase-2 induction regulates cadmium-induced autophagy in kidney vol.7, pp.6, 2016, https://doi.org/10.1038/cddis.2016.78
  2. Lengths of nephron tubule segments and collecting ducts in the CD-1 mouse kidney: an ontogeny study vol.311, pp.5, 2016, https://doi.org/10.1152/ajprenal.00435.2016
  3. Flavocoxid, a Natural Antioxidant, Protects Mouse Kidney from Cadmium-Induced Toxicity vol.2018, pp.1942-0994, 2018, https://doi.org/10.1155/2018/9162946