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Toothpick-Aided Detection of Sclerotinia homoeocarpa in the Turfgrass Leaf Canopy, Thatch, and Soil in Relation to Dollar Spot Infection Centers

이쑤시개를 이용한 잔디층, 대취층, 및 토양층에서 동전마름병 전염원의 검출

  • Lee, Jung Han (Korea Turfgrass Research Institute) ;
  • Min, Gyu Young (Daejung Golf Engineering Co. Ltd.) ;
  • Shim, Gyu Yul (Korea Turfgrass Research Institute) ;
  • Kim, Dong Soo (Southern Forest Resource Research Center, Korea Forestry Research Institute) ;
  • Sang, Hyunkyu (Stockbridge School of Agriculture, University of Massachusetts) ;
  • Jung, Geunhwa (Stockbridge School of Agriculture, University of Massachusetts) ;
  • Kwak, Youn-Sig (Department of Plant Medicine and IALS, Gyeongsang National University)
  • 이정한 (한국잔디연구소) ;
  • 민규영 (대정골프엔지니어링) ;
  • 심규열 (한국잔디연구소) ;
  • 김동수 (국립산림과학원 남부산림자원연구소) ;
  • 상현규 (매사추세스대학교 스탁브릿지 농업대학) ;
  • 정근화 (매사추세스대학교 스탁브릿지 농업대학) ;
  • 곽연식 (경상대학교 식물의학과)
  • Received : 2015.02.03
  • Accepted : 2015.03.16
  • Published : 2015.12.30

Abstract

Dollar spot, caused by Sclerotinia homoeocarpa, is the major disease in cool-season turfgrasses. Understanding the distribution of this pathogen in soil and thatch is important to developing disease control strategies. In this study, toothpicks were used to detect S. homoeocarpa in the turfgrass canopy, thatch, and soil at different distances from dollar spot infection centers. The effect of penetrant and contact fungicide applications with different water volumes on distribution of S. homoeocarpa was also investigated. S. homoeocarpa was detected in 100% of samples taken from the leaf canopy, 83.3% in thatch area, and 0% in the soil from within the infection center. S. homoeocarpa was isolated in 100% of samples taken from the edge of the infection center, but was only detected in 13% of the samples taken at 1.5 cm away from the infection center edge. S. homoeocarpa was isolated at a higher frequency in the propiconazole treated plots than those treated with chlorothalonil and was not detected in leaf canopy samples when either fungicides was applied with 6.78 L of water. In conclusion, the toothpick-aided detection technique has improved our understanding of S. homoeocarpa epidemiology and could be used as a diagnostic tool to detect for fungicide resistance on golf courses.

Acknowledgement

Supported by : Rural Development Administration

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