- Volume 57 Issue 2
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Discrimination of the commercial Korean native chicken population using microsatellite markers
- Choi, Nu Ri (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
- Seo, Dong Won (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
- Jemaa, Slim Ben (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
- Sultana, Hasina (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
- Heo, Kang Nyeong (Poultry Science Division, National Institute of Animal Science, RDA) ;
- Jo, Cheorun (Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Science, Seoul National University) ;
- Lee, Jun Heon (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
- Received : 2014.12.23
- Accepted : 2015.01.20
- Published : 2015.02.28
Background: Korean native chicken (KNC) is a well-known breed due to its superior meat taste. This breed, however, owing to a low growth rate, has a high market price. In order to overcome this disadvantage, the National Institute of Animal Science (NIAS) in Korea developed a commercial KNC breed, named Woorimatdag version 2 (WM2), an upgraded version of the Woorimatdag (WM1) breed and the WM2 was created by crossing the KNC with meat type breeds. This study aims to discriminate between WM2 and other chicken breeds using microsatellite (MS) markers. Methods: A total of 302 individuals from eight Korean chicken populations were examined. The genetic diversity and population structure analysis were investigated using Cervus, API-CALC, STRUCTURE, PowerMarker programs. Results: Based on heterozygosity and polymorphic information content (PIC) values, 30 MS markers were initially selected from 150 markers. The identified average number of alleles (Na), expected heterozygosity, and PIC values for the WM2 samples were 7.17, 0.741, and 0.682, respectively. Additionally, the paternity of individuals was assigned with a success rate of greater than 99% using 12 markers, the best minimum number of markers. The 12 selected markers contained heterozygosity and PIC values above 0.7 and probability of identity values around zero. Using these markers, the determined probability of identity (PI),
Supported by : Korea Institute of Planning & Evaluation for Technology in Food, Agriculture Forestry & Fisheries (IPET)
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