Immunopreventive Effects against Murine H22 Hepatocellular Carcinoma in vivo by a DNA Vaccine Targeting a Gastrin-Releasing Peptide

  • Meko'o, Jean Louis Didier (School of Life Science and Technology, China Pharmaceutical University) ;
  • Xing, Yun (School of Life Science and Technology, China Pharmaceutical University) ;
  • Zhang, Huiyong (School of Life Science and Technology, China Pharmaceutical University) ;
  • Lu, Yong (School of Life Science and Technology, China Pharmaceutical University) ;
  • Wu, Jie (School of Life Science and Technology, China Pharmaceutical University) ;
  • Cao, Rongyue (School of Life Science and Technology, China Pharmaceutical University)
  • Published : 2014.11.06


There is a continuing need for innovative alternative therapies for liver cancer. DNA vaccines for hormone/growth factor immune deprivation represent a feasible and attractive approach for cancer treatment. We reported a preventive effect of a DNA vaccine based on six copies of the B cell epitope GRP18-27 with optimized adjuvants against H22 hepatocarcinoma. Vaccination with pCR3.1-VS-HSP65-TP-GRP6-M2 (vaccine) elicited much higher level of anti-GRP antibodies and proved efficacious in preventing growth of transplanted hepatocarcinoma cells. The tumor size and weight were significantly lower (p<0.05) in the vaccine subgroup than in the control pCR3.1-VS-TP-HSP65-TP-GRP6, pCR3.1-VS-TP-HSP65-TP-M2 or saline subgroups. In addition, significant reduction of tumor-induced angiogenesis associated with intradermal tumors of H22 cells was observed. These potent effects may open ways towards the development of new immunotherapeutic approaches in the treatment of liver cancer.


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