DOI QR코드

DOI QR Code

Comparison between Effects of Free Curcumin and Curcumin Loaded NIPAAm-MAA Nanoparticles on Telomerase and PinX1 Gene Expression in Lung Cancer Cells

  • Badrzadeh, Fariba ;
  • Akbarzadeh, Abolfazl ;
  • Zarghami, Nosratollah ;
  • Yamchi, Mohammad Rahmati ;
  • Zeighamian, Vahide ;
  • Tabatabae, Fateme Sadatem ;
  • Taheri, Morteza ;
  • Kafil, Hossein Samadi
  • Published : 2014.11.06

Abstract

Background: Herbal compounds such as curcumin which decrease telomerase and gene expression have been considered as beneficial tools for lung cancer treatment. In this article, we compared the effects of pure curcumin and curcumin-loaded NIPAAm-MAA nanoparticles on telomerase and PinX1 gene expression in a lung cancer cell line. Materials and Methods: A tetrazolium-based assay was used for determination of cytotoxic effects of curcumin on the Calu-6 lung cancer cell line and telomerase and pinX1 gene expression was measured with real-time PCR. Results: MTT assay showed that Curcumin-loaded NIPAAm-MAA inhibited the growth of the Calu-6 lung cancer cell line in a time and dose-dependent manner. Our q-PCR results showed that the expression of telomerase gene was effectively reduced as the concentration of curcumin-loaded NIPAAm-MAA increased while expression of the PinX1 gene became elevated. Conclusions: The results showed that curcumin-loaded-NIPAAm-MAA exerted cytotoxic effects on the Calu-6 cell line through down-regulation of telomerase and stimulation of pinX1 gene expression. NIPPAm-MAA could be good carrier for such kinds of hydrophobic agent.

Keywords

Curcumin;NIPAAm-MAA;PinX1;telomerase;lung cancer cells

References

  1. Aggarwal BB, Shishodia S, Takada Y, et al (2005). Curcumin suppresses the paclitaxel-induced nuclear factor-$\kappa{B}$ pathway in breast cancer cells and inhibits lung metastasis of human breast cancer in nude mice. Clin Cancer Res, 11, 7490-98. https://doi.org/10.1158/1078-0432.CCR-05-1192
  2. Azarmi S, Roa WH, Lobenberg R (2008). Targeted delivery of nanoparticles for the treatment of lung diseases. Adv Drug Deliv Rev, 60, 863-75. https://doi.org/10.1016/j.addr.2007.11.006
  3. Bach PB, Cramer LD, Warren JL, Begg CB (1999). Racial differences in the treatment of early-stage lung cancer. N Engl J Med, 341, 1198-205. https://doi.org/10.1056/NEJM199910143411606
  4. Brown JS, Eraut D, Trask C, Davison AG (1996). Age and the treatment of lung cancer. Thorax, 51, 564-68. https://doi.org/10.1136/thx.51.6.564
  5. Cong YS, Wright WE, Shay JW (2002). Human telomerase and its regulation. Microbiol Mol Biol Rev, 66, 407-25. https://doi.org/10.1128/MMBR.66.3.407-425.2002
  6. Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA: A cancer journal for clinicians, 61, 69-90. https://doi.org/10.3322/caac.20107
  7. Davaran S, Alimirzalu S, Nejati-Koshki K, et al (2014). Physicochemical characteristics of $Fe_3O_4$ magnetic nanocomposites based on poly (N-isopropylacrylamide) for anti-cancer drug delivery. Asian Pac J Cancer Prev, 15, 49-54. https://doi.org/10.7314/APJCP.2014.15.1.49
  8. Gupta P, Vermani K,Garg S (2002). Hydrogels: from controlled release to pH-responsive drug delivery. Drug Discov Today, 7, 569-79. https://doi.org/10.1016/S1359-6446(02)02255-9
  9. Hosseini M, Naghan PA, Karimi S, et al (2009). Environmental risk factors for lung cancer in Iran: a case-control study. Int J Epidemiol, 38, 989-96. https://doi.org/10.1093/ije/dyp218
  10. Kazemi-Lomedasht F, Rami A, Zarghami N (2013). Comparison of inhibitory effect of curcumin nanoparticles and free curcumin in human telomerase reverse transcriptase gene expression in breast cancer. Adv Pharm Bull, 3, 127-130.
  11. Li PM, Li YL, Liu B, et al (2014). Curcumin inhibits MHCC97H liver cancer cells by activating ROS/TLR-4/caspase signaling pathway. Asian Pac J Cancer Prev, 15, 2329-34. https://doi.org/10.7314/APJCP.2014.15.5.2329
  12. Li Y, Zhang S, Geng JX, Hu XY (2013). Curcumin inhibits human non-small cell lung cancer A549 cell proliferation through regulation of Bcl-2/Bax and cytochrome C. Asian Pac J Cancer Prev, 14, 4599-602. https://doi.org/10.7314/APJCP.2013.14.8.4599
  13. Liang G, Yang S, Zhou H, et al (2009). Synthesis, crystal structure and anti-inflammatory properties of curcumin analogues. Eur J Med Chem, 44, 915-19. https://doi.org/10.1016/j.ejmech.2008.01.031
  14. Molina JR, Yang P, Cassivi SD, Schild SE, Adjei AA (2008). Non-small cell lung cancer: epidemiology, risk factors, treatment, and survivorship. Mayo Clin Proc, 83, 584-94. https://doi.org/10.1016/S0025-6196(11)60735-0
  15. Tian P, Wu Q, Lian K (2008). Preparation of temperature- and pHsensitive, stimuli-responsive poly (N-isopropylacrylamideco-methacrylic acid) nanoparticles. J Applied Polymer Science, 108, 2226-32. https://doi.org/10.1002/app.27888
  16. Motterlini R, Foresti R, Bassi R,Green CJ (2000). Curcumin, an antioxidant and anti-inflammatory agent, induces heme oxygenase-1 and protects endothelial cells against oxidative stress. Free Radic Biol Med, 28, 1303-12. https://doi.org/10.1016/S0891-5849(00)00294-X
  17. Nasiri M, Zarghami N, Koshki KN, et al (2013). Curcumin and silibinin inhibit telomerase expression in T47D human breast cancer cells. Asian Pac J Cancer Prev, 14, 3449-53. https://doi.org/10.7314/APJCP.2013.14.6.3449
  18. Ramachandran C, Rodriguez S, Ramachandran R, et al (2005). Expression profiles of apoptotic genes induced by curcumin in human breast cancer and mammary epithelial cell lines. Anticancer Res, 25, 3293-302.
  19. Wang B, Xu XD, Wang ZC, et al (2008). "Synthesis and properties of pH and temperature sensitive P (NIPAAmco-DMAEMA) hydrogels. Colloids Surf B Biointerfaces, 64, 34-41. https://doi.org/10.1016/j.colsurfb.2008.01.001
  20. Yadav D, Anwar MF, Garg V, et al (2014). Development of polymeric nanopaclitaxel and comparison with free paclitaxel for effects on cell proliferation of MCF-7 and B16F0 carcinoma cells. Asian Pac J Cancer Prev, 15, 2335-40. https://doi.org/10.7314/APJCP.2014.15.5.2335
  21. Yoder LH (2006). Lung cancer epidemiology. Medsurg Nursing, 15, 171.

Cited by

  1. Magnetic nanoparticles: Applications in gene delivery and gene therapy pp.2169-141X, 2015, https://doi.org/10.3109/21691401.2015.1014093
  2. Comparison, synthesis and evaluation of anticancer drug-loaded polymeric nanoparticles on breast cancer cell lines pp.2169-141X, 2015, https://doi.org/10.3109/21691401.2015.1008510
  3. Deactivation of Telomerase Enzyme and Telomere Destabilization by Natural Products: a Potential Target for Cancer Green Therapy vol.16, pp.18, 2016, https://doi.org/10.7314/APJCP.2015.16.18.8671
  4. Progress in nanotechnology-based drug carrier in designing of curcumin nanomedicines for cancer therapy: current state-of-the-art vol.24, pp.4, 2016, https://doi.org/10.3109/1061186X.2015.1055570
  5. Upregulation of cugbp2 increases response of pancreatic cancer cells to chemotherapy vol.401, pp.1, 2016, https://doi.org/10.1007/s00423-015-1364-1
  6. Emerging Importance of Phytochemicals in Regulation of Stem Cells Fate via Signaling Pathways vol.31, pp.11, 2017, https://doi.org/10.1002/ptr.5908
  7. Silibinin-loaded magnetic nanoparticles inhibit hTERT gene expression and proliferation of lung cancer cells vol.45, pp.8, 2017, https://doi.org/10.1080/21691401.2016.1276922
  8. Co-Delivery of Curcumin and Chrysin by Polymeric Nanoparticles Inhibit Synergistically Growth and hTERT Gene Expression in Human Colorectal Cancer Cells vol.69, pp.8, 2017, https://doi.org/10.1080/01635581.2017.1367932
  9. Macrophage repolarization using CD44-targeting hyaluronic acid–polylactide nanoparticles containing curcumin pp.2169-141X, 2017, https://doi.org/10.1080/21691401.2017.1408116
  10. Nano-encapsulated metformin-curcumin in PLGA/PEG inhibits synergistically growth and hTERT gene expression in human breast cancer cells pp.2169-141X, 2017, https://doi.org/10.1080/21691401.2017.1347879
  11. Telomerase Inhibitors from Natural Products and Their Anticancer Potential vol.19, pp.1, 2017, https://doi.org/10.3390/ijms19010013
  12. Current developments in green synthesis of metallic nanoparticles using plant extracts: a review pp.2169-141X, 2018, https://doi.org/10.1080/21691401.2018.1492931
  13. Latest in Vitro and in Vivo Assay, Clinical Trials and Patents in Cancer Treatment using Curcumin: A Literature Review vol.70, pp.4, 2018, https://doi.org/10.1080/01635581.2018.1464347
  14. gene expression in mouse B16F10 melanoma tumour model pp.2169-141X, 2018, https://doi.org/10.1080/21691401.2018.1452021
  15. Curcumin as a therapeutic agent in leukemia pp.00219541, 2019, https://doi.org/10.1002/jcp.28072
  16. The relationship between microRNAs and Rab family GTPases in human cancers pp.00219541, 2019, https://doi.org/10.1002/jcp.28038
  17. Synthesis and characterization of PEG-functionalized graphene oxide as an effective pH-sensitive drug carrier vol.47, pp.1, 2019, https://doi.org/10.1080/21691401.2018.1543196