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Potential Mechanisms of Benzyl Isothiocyanate Suppression of Invasion and Angiogenesis by the U87MG Human Glioma Cell Line

  • Zhu, Yu (Department of Clinical Laboratory, Tianjin Huan Hu Hospital, Tianjin Key Laboratory of Cerebral Vessels and Neural Degeneration) ;
  • Zhang, Ling (Department of Clinical Laboratory, Tianjin Huan Hu Hospital, Tianjin Key Laboratory of Cerebral Vessels and Neural Degeneration) ;
  • Zhang, Guo-Dong (Department of Clinical Laboratory, Tianjin Huan Hu Hospital, Tianjin Key Laboratory of Cerebral Vessels and Neural Degeneration) ;
  • Wang, Hong-Ou (Public Health Department, Tianjin Medical University) ;
  • Liu, Ming-Yan (Department of Oncology, Tianjin Central Hospital of Gynecology Obstetrics) ;
  • Jiang, Yuan (Department of Oncology, Tianjin Central Hospital of Gynecology Obstetrics) ;
  • Qi, Li-Sha (Key Laboratory of Immune Microenvironment and Disease of the Ministry of Education, Tianjin Medical University) ;
  • Li, Qi (Key Laboratory of Immune Microenvironment and Disease of the Ministry of Education, Tianjin Medical University) ;
  • Yang, Ping (Department of Clinical Laboratory, Tianjin Huan Hu Hospital, Tianjin Key Laboratory of Cerebral Vessels and Neural Degeneration)
  • Published : 2014.10.23

Abstract

Glioma is one of the most common tumors in China and chemotherapy is critical for its treatment. Recent studies showed that benzyl isothiocyanate (BITC) could inhibit the growth of glioma cells, but the mechanisms are not fully understood. This study explored the inhibitory effect of BITC on invasion and angiogenesis of U87MG human glioma cells in vitro and in vivo, as well as potential mechanisms. It was found that BITC could inhibit invasion and angiogenesis of human glioma U87MG cells by inducing cell cycle arrest at phase G2/M. It also was demonstrated that BITC decreased expression of cyclin B1, p21, MMP-2/9, VE-cadherin, CD44, CXCR4 and MTH1, the activity of the telomerase and $PKC{\zeta}$ pathway. Microarray analysis was thus useful to explore the potential target genes related to tumorigenic processes. BITC may play important roles in the inhibition of invasion and angiogenesis of human glioma cells.

Keywords

Glioma cells;benzyl isothiocyanate;invasion;angiogenesis

Acknowledgement

Supported by : The Fund of the Health Bureau of Tianjin

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