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Correlation between E-Cadherin-Regulated Cell Adhesion and Human Osteosarcoma MG-63 Cell Anoikis

  • Lin, Ding-Sheng (Department of Orthopedics, the 2nd Affiliated Hospital of Wenzhou Medical University, Key Lab of Zhejiang Orthopedics Science) ;
  • Cai, Le-Yi (Department of Orthopedics, the 2nd Affiliated Hospital of Wenzhou Medical University, Key Lab of Zhejiang Orthopedics Science) ;
  • Ding, Jian (Department of Orthopedics, the 2nd Affiliated Hospital of Wenzhou Medical University, Key Lab of Zhejiang Orthopedics Science) ;
  • Gao, Wei-Yang (Department of Orthopedics, the 2nd Affiliated Hospital of Wenzhou Medical University, Key Lab of Zhejiang Orthopedics Science)
  • Published : 2014.10.23

Abstract

Purpose: The aim of this study was to investigate the relationship between cell adhesion and anoikis evasion among human osteosarcoma cells (MG-63), and to further study the molecular mechanisms. Materials and Methods: Human osteosarcoma cells (MG-63) were assessed for apoptosis, and caspase-3, E-cadherin and ${\beta}$-catenin expression in EDTA and control non-EDTA groups. Results: MG-63 cells were predominantly aggregated when in suspension, and the suspended cells were more dispersed in the EDTA group. Following culture in suspension for 24 h, 48 h, or 72 h, the rates of apoptosis were $34.88%{\pm}3.64%$, $59.3%{\pm}7.22%$ and $78.5%{\pm}5.21%$ in the experimental group and $7.34%{\pm}2.13%$, $14.7%{\pm}3.69%$, and $21.4%{\pm}3.60%$ in the control group, respectively. Caspase-3 expression progressively increased and E-cadherin and ${\beta}$-catenin were decreased in the experimental group, whereas there was no change in the control group. Conclusions: MG-63 cells could avoid anoikis through cell adhesion, and E-cadherin might play a role in this process.

Keywords

Osteosarcoma;anoikis;cell adhesion;E-cadherin

Acknowledgement

Supported by : ZheJiang Provincial Natural Science Foundation

References

  1. Cao L, Zhang Z, Han L, et al (2014). Mitogen-activated protein kinase pathway is pivotal for anoikis resistance in metastatic hepatoma cells. Mol Med Rep, 9, 1121-7.
  2. Asiaf A, Ahmad ST, Aziz SA, et al (2014). Loss of expression and aberrant methylation of the CDH1 (E-cadherin) gene in breast cancer patients from Kashmir. Asian Pac J Cancer Prev, 15, 6397-403. https://doi.org/10.7314/APJCP.2014.15.15.6397
  3. Burnette DT, Shao L, Ott C, et al (2014). A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells. J Cell Biol, 205, 83-96. https://doi.org/10.1083/jcb.201311104
  4. Chang HW, Wang HC, Chen CY, et al (2014). 5-azacytidine induces anoikis, inhibits mammosphere formation and reduces metalloproteinase 9 activity in MCF-7 human breast cancer cells. Molecules, 19, 3149-59. https://doi.org/10.3390/molecules19033149
  5. Chaotham C, Pongrakhananon V, Sritularak B, Chanvorachote P (2014). A bibenzyl from dendrobium ellipsophyllum inhibits epithelial-to-mesenchymal transition and sensitizes lung cancer cells to anoikis. Anticancer Res, 34, 1931-8.
  6. He ML, Wu Y, Zhao JM, et al (2013). PIK3CA and AKT gene polymorphisms in susceptibility to osteosarcoma in a Chinese population. Asian Pac J Cancer Prev, 14, 5117-22. https://doi.org/10.7314/APJCP.2013.14.9.5117
  7. Fan M, Sun J, Wang W, et al (2014). Tropomyosin-related kinase B promotes distant metastasis of colorectal cancer through protein kinase B-mediated anoikis suppression and correlates with poor prognosis. Apoptosis, 19, 860-70. https://doi.org/10.1007/s10495-014-0968-1
  8. Frisch SM, Screaton RA (2001). Anoikis mechanisms. Curr Opin Cell Biol, 13, 555-62. https://doi.org/10.1016/S0955-0674(00)00251-9
  9. He JP, Hao Y, Wang XL, et al (2014). Review of the molecular pathogenesis of osteosarcoma. Asian Pac J Cancer Prev, 15, 5967-76. https://doi.org/10.7314/APJCP.2014.15.15.5967
  10. Hynes RO (1992). Integrins: versatility, modulation, and signaling in cell adhesion. Cell, 69, 11-25. https://doi.org/10.1016/0092-8674(92)90115-S
  11. Ivanova IA, Vermeulen JF, Ercan C, et al (2013). FER kinase promotes breast cancer metastasis by regulating ${\alpha}6$- and ${\beta}1$-integrin-dependent cell adhesion and anoikis resistance. Oncogene, 32, 5582-92. https://doi.org/10.1038/onc.2013.277
  12. Jin J, Cai L, Liu ZM, et al (2013). MiRNA-218 inhibits osteosarcoma cell migration and invasion by downregulating of TIAM1, MMP2 and MMP9. Asian Pac J Cancer Prev, 14, 3681-4. https://doi.org/10.7314/APJCP.2013.14.6.3681
  13. Kanda Y, Kawaguchi T, Kuramitsu Y, et al (2014). Fascin regulates chronic inflammation-related human colon carcinogenesis by inhibiting cell anoikis. Protenomics, 14, 1031-41. https://doi.org/10.1002/pmic.201300414
  14. Kang HG, Jenabi JM, Zhang J, et al (2007). E-cadherin cellcell adhesion in ewing tumor cells mediates suppression of anoikis through activation of the ErbB4 tyrosine kinase. Cancer Res, 67, 3094-105. https://doi.org/10.1158/0008-5472.CAN-06-3259
  15. Lee JW, Kim JH (2013). Activation of the leukotriene B4 receptor 2-reactive oxygen species (BLT2-ROS) cascade following detachment confers anoikis resistance in prostate cancer cells. J Biol Chem, 288, 30054-63. https://doi.org/10.1074/jbc.M113.481283
  16. Marco RA, Diaz-Montero CM, et al (2003). Alpha 4 integrin increases anoikis of human osteosarcoma cells. J Cell Biochem, 88, 1038-47. https://doi.org/10.1002/jcb.10465
  17. Lin D, Feng J, Chen W (2008). Bcl-2 and caspase-8 related anoikis resistance in human osteosarcoma MG-63 cells. Cell Biol Int, 32, 1199-206. https://doi.org/10.1016/j.cellbi.2008.07.002
  18. Lin D (2011). Possible mechanism of anoikis resistance in human osteosarcome MG-63 cells. Chinese J Pathophysiol, 27, 165-70.
  19. Ma XK, Wang L, Li Y, et al (2010). HAb18G/CD147 cell-cell contacts confer resistance of a HEK293 subpopulation to anoikis in an E-cadherin-dependent manner. BMC Cell Biol, 11, 27. https://doi.org/10.1186/1471-2121-11-27
  20. Maroni P, Bendinelli P, Matteucci E, et al (2014). Osteolytic bone metastasis is hampered by impinging on the interplay among autophagy, anoikis and ossification. Cell Death Dis, 5, 1005. https://doi.org/10.1038/cddis.2013.465
  21. Onder TT, Gupta PB, Mani SA, et al (2008). Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. Cancer Res, 68, 3645-54. https://doi.org/10.1158/0008-5472.CAN-07-2938
  22. Paoli P, Giannoni E, Chiarugi P (2013). Anoikis molecular pathways and its role in cancer progression. Biochim Biophys Acta, 1833, 3481-98. https://doi.org/10.1016/j.bbamcr.2013.06.026
  23. Perry JM, He XC, Sugimura R, et al (2011). Cooperation between both wnt/{beta}-catenin and PTEN/PI3K/Akt signaling promotes primitive hematopoietic stem cell self-renewal and expansion. Genes Dev, 25, 1928-42. https://doi.org/10.1101/gad.17421911
  24. Regezi JA, Ramos DM, Pytela R, et al (2002). Tenascin and beta 6 integrin are overexpressed in floor of mouth in situ carcinomas and invasive squamous cell carcinomas. Oral Oncol, 38, 332-6. https://doi.org/10.1016/S1368-8375(01)00062-8
  25. Silginer M, Weller M, Ziegler U, Roth P (2014). Integrin inhibition promotes atypical anoikis in glioma cells. Cell Death Dis, 5, 1012. https://doi.org/10.1038/cddis.2013.543
  26. Strauss SJ, Ng T, Mendoza-Naranjo A, et al (2010). Understanding micrometastatic disease and anoikis resistance in ewing family of tumors and osteosarcoma. Oncologist, 15, 627-35. https://doi.org/10.1634/theoncologist.2010-0093
  27. Su CY, Li YS, Han Y, et al (2014). Correlation between expression of cell adhesion molecules CD44v6 and E-cadherin and lymphatic metastasis in non- small cell lung cancer. Asian Pac J Cancer Prev, 15, 2221-4. https://doi.org/10.7314/APJCP.2014.15.5.2221
  28. Tamura G, Yin J, Wang S, et al (2000). E-cadherin gene promoter hypermethylation in primary human gastric carcinomas. J Natl Cancer Inst, 92, 569-73. https://doi.org/10.1093/jnci/92.7.569
  29. Tu CL, You M (2014). Obligatory roles of filamin A in E-cadherin-mediated cell-cell adhesion in epidermal keratinocytes. J Dermatol Sci, 73, 142-51. https://doi.org/10.1016/j.jdermsci.2013.09.007
  30. Wang XS, Luo KJ, Bella AE, et al (2014). Caspase-3 expression in metastatic lymph nodes of esophageal squamous cell carcinoma is prognostic of survival. World J Gastroenterol, 20, 4414-20. https://doi.org/10.3748/wjg.v20.i15.4414
  31. Zhang Y, Wang CP, Ding XX, et al (2014). FNC, a novel nucleoside analogue, blocks invasion of aggressive nonhodgkin lymphoma cell lines via inhibition of the Wnt/$\beta$-catenin signaling pathway. Asian Pac J Cancer Prev, 15, 6829-35. https://doi.org/10.7314/APJCP.2014.15.16.6829

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