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Flavonoids of Rosa roxburghii Tratt Act as Radioprotectors

  • Xu, Ping (Department of Pharmacy, Xinxiang Medical University) ;
  • Zhang, Wen-Bo (Department of Pharmacy, Xinxiang Medical University) ;
  • Cai, Xin-Hua (Department of Pharmacy, Xinxiang Medical University) ;
  • Lu, Dan-Dan (Institute of Radiation Medicine, Military Medical Sciences) ;
  • He, Xiao-Yang (Institute of Radiation Medicine, Military Medical Sciences) ;
  • Qiu, Pei-Yong (Department of Pharmacy, Xinxiang Medical University) ;
  • Wu, Jiao (Department of Pharmacy, Xinxiang Medical University)
  • Published : 2014.10.23

Abstract

Background: To study the radioprotective effects of flavonoids from Rosa roxburghii Tratt (FRT). Materials and Methods: The radioprotective effects of FRT were investigated by examining cell viability, 30-day survival of mice and the number of colony-forming units in spleen (CFU-S) after total-body 60Co irradiation. Results: The survival rates of irradiated cells gradually increased with increasing concentrations of FRT. The survival rate was the highest at 87% with a concentration of $30{\mu}g/mL$. Pretreatment with FRT was needed to realize its radioprotective activity in mice at the dose of 60 mg/kg. With the increasing doses of 30 mg/kg, 60 mg/kg and 120 mg/kg, the numbers of CFU-S increased, and were significantly different compared with the control group. Conclusions: Pretreatment with FRT prior to irradiation resulted in significantly higher cell survival at 24 h after 5 Gy radiation, increased 30-day survival in mice after exposure to a potentially lethal dose of 8 Gy, and resulted in a higher number of CFU-S in mice after exposure to a dose of 6 Gy. These results collectively indicate that FRT is an effective radioprotective agent.

Keywords

FRT;radioprotection;survival rate;AHH-1;CFU-S

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