DOI QR코드

DOI QR Code

Assessment of interleukin-1beta and interleukin-6 in the crevicular fluid around healthy implants, implants with peri-implantitis, and healthy teeth: a cross-sectional study

  • Received : 2014.06.04
  • Accepted : 2014.08.27
  • Published : 2014.10.31

Abstract

Objectives: This study aimed to assess and compare the levels of interleukin-1beta (IL-$1{\beta}$) and interleukin-6 (IL-6) in the crevicular fluid around healthy implants, implants with peri-implantitis, and healthy teeth. Materials and Methods: This study evaluated 16 dental implants in 8 patients (4 males and 4 females). These patients had at least one healthy implant and one implant with peri-implantitis next to healthy teeth. The crevicular fluid was collected using absorbent cones and transferred to the laboratory. Specimens were evaluated by ELISA for interleukin levels. Data were analyzed using repeated measures ANOVA and Bonferroni tests (P<0.05). Results: Levels of IL-$1{\beta}$ in the crevicular fluid around implants with peri-implantitis were significantly higher than around healthy implants (P=0.002); the latter was significantly higher than around healthy teeth (P=0.015). A significant difference was found in the level of IL-6 in the crevicular fluid around implants with peri-implantitis and healthy implants (P=0.049) and also between implants with peri-implantitis and healthy teeth (P<0.001). Conclusion: Within the limitations of this study, significant differences exist in the levels of IL-$1{\beta}$ and IL-6 in the crevicular fluid of implants with peri-implantitis, healthy implants, and healthy teeth. More studies with larger sample sizes in different populations are necessary.

References

  1. Abrishami MR, Sabour S, Nasiri M, Amid R, Kadkhodazadeh M. Comparison of the reproducibility of results of a new peri-implantitis assessment system (implant success index) with the Misch classification. J Korean Assoc Oral Maxillofac Surg 2014;40:61-7. https://doi.org/10.5125/jkaoms.2014.40.2.61
  2. Roos-Jansaker AM, Lindahl C, Renvert H, Renvert S. Nine- to fourteen-year follow-up of implant treatment. Part II: presence of peri-implant lesions. J Clin Periodontol 2006;33:290-5. https://doi.org/10.1111/j.1600-051X.2006.00906.x
  3. Ata-Ali J, Flichy-Fernandez AJ, Ata-Ali F, Penarrocha-Diago M, Penarrocha-Diago M. Clinical, microbiologic, and host response characteristics in patients with peri-implant mucositis. Int J Oral Maxillofac Implants 2013;28:883-90. https://doi.org/10.11607/jomi.2654
  4. Petkovic AB, Matic SM, Stamatovic NV, Vojvodic DV, Todorovic TM, Lazic ZR, et al. Proinflammatory cytokines (IL-1beta and TNF-alpha) and chemokines (IL-8 and MIP-1alpha) as markers of peri-implant tissue condition. Int J Oral Maxillofac Surg 2010;39:478-85. https://doi.org/10.1016/j.ijom.2010.01.014
  5. Sanz M, Chapple IL; Working Group 4 of the VIII European Workshop on Periodontology. Clinical research on peri-implant diseases: consensus report of Working Group 4. J Clin Periodontol 2012;39(Suppl 12):202-6. https://doi.org/10.1111/j.1600-051X.2011.01837.x
  6. Greenstein G, Caton J. Periodontal disease activity: a critical assessment. J Periodontol 1990;61:543-52. https://doi.org/10.1902/jop.1990.61.9.543
  7. Dennison DK, Van Dyke TE. The acute inflammatory response and the role of phagocytic cells in periodontal health and disease. Periodontol 2000 1997;14:54-78. https://doi.org/10.1111/j.1600-0757.1997.tb00192.x
  8. Okada H, Murakami S. Cytokine expression in periodontal health and disease. Crit Rev Oral Biol Med 1998;9:248-66. https://doi.org/10.1177/10454411980090030101
  9. Panagakos FS, Aboyoussef H, Dondero R, Jandinski JJ. Detection and measurement of inflammatory cytokines in implant crevicular fluid: a pilot study. Int J Oral Maxillofac Implants 1996;11:794-9.
  10. Stashenko P, Fujiyoshi P, Obernesser MS, Prostak L, Haffajee AD, Socransky SS. Levels of interleukin 1 beta in tissue from sites of active periodontal disease. J Clin Periodontol 1991;18:548-54. https://doi.org/10.1111/j.1600-051X.1991.tb00088.x
  11. Ataoglu H, Alptekin NO, Haliloglu S, Gursel M, Ataoglu T, Serpek B, et al. Interleukin-1beta, tumor necrosis factor-alpha levels and neutrophil elastase activity in peri-implant crevicular fluid. Clin Oral Implants Res 2002;13:470-6. https://doi.org/10.1034/j.1600-0501.2002.130505.x
  12. Kadkhodazadeh M, Esfahrood ZR, Amid R, Zarnegarnia P. Comparison of the acceptability of a new scoring system with Misch's classification for dental implant success determination. J Long Term Eff Med Implants 2012;22:85-93. https://doi.org/10.1615/JLongTermEffMedImplants.v22.i1.90
  13. Griffiths GS, Curtis MA, Wilton JM. Selection of a filter paper with optimum properties for the collection of gingival crevicular fluid. J Periodontal Res 1988;23:33-8. https://doi.org/10.1111/j.1600-0765.1988.tb01024.x
  14. Page RC. Host response tests for diagnosing periodontal diseases. J Periodontol 1992;63(4 Suppl):356-66. https://doi.org/10.1902/jop.1992.63.4s.356
  15. Casado PL, Canullo L, de Almeida Filardy A, Granjeiro JM, Barboza EP, Leite Duarte ME. Interleukins 1${\beta}$ and 10 expressions in the periimplant crevicular fluid from patients with untreated periimplant disease. Implant Dent 2013;22:143-50. https://doi.org/10.1097/ID.0b013e3182818792
  16. Nowzari H, Botero JE, DeGiacomo M, Villacres MC, Rich SK. Microbiology and cytokine levels around healthy dental implants and teeth. Clin Implant Dent Relat Res 2008;10:166-73. https://doi.org/10.1111/j.1708-8208.2007.00076.x
  17. Murata M, Tatsumi J, Kato Y, Suda S, Nunokawa Y, Kobayashi Y, et al. Osteocalcin, deoxypyridinoline and interleukin-1beta in peri-implant crevicular fluid of patients with peri-implantitis. Clin Oral Implants Res 2002;13:637-43. https://doi.org/10.1034/j.1600-0501.2002.130610.x
  18. Melo RF, Lopes BM, Shibli JA, Marcantonio E Jr, Marcantonio RA, Galli GM. Interleukin-1${\beta}$ and interleukin-6 expression and gene polymorphisms in subjects with peri-implant disease. Clin Implant Dent Relat Res 2012;14:905-14. https://doi.org/10.1111/j.1708-8208.2010.00325.x
  19. Stashenko P, Dewhirst FE, Peros WJ, Kent RL, Ago JM. Synergistic interactions between interleukin 1, tumor necrosis factor, and lymphotoxin in bone resorption. J Immunol 1987;138:1464-8.
  20. Preiss DS, Meyle J. Interleukin-1 beta concentration of gingival crevicular fluid. J Periodontol 1994;65:423-8. https://doi.org/10.1902/jop.1994.65.5.423
  21. Zhong Y, Slade GD, Beck JD, Offenbacher S. Gingival crevicular fluid interleukin-1beta, prostaglandin E2 and periodontal status in a community population. J Clin Periodontol 2007;34:285-93. https://doi.org/10.1111/j.1600-051X.2007.01057.x
  22. Guncu GN, Akman AC, Gunday S, Yamalik N, Berker E. Effect of inflammation on cytokine levels and bone remodelling markers in peri-implant sulcus fluid: a preliminary report. Cytokine 2012;59:313-6. https://doi.org/10.1016/j.cyto.2012.04.024
  23. Yaghobee S, Khorsand A, Paknejad M. Comparison of interleukin-1${\beta}$ levels in gingival crevicular fluid and peri-implant crevicular fluid and its relationship with clinical indexes. J Dent (Tehran) 2013;10:1-9.
  24. Liskmann S, Vihalemm T, Salum O, Zilmer K, Fischer K, Zilmer M. Correlations between clinical parameters and interleukin-6 and interleukin-10 levels in saliva from totally edentulous patients with peri-implant disease. Int J Oral Maxillofac Implants 2006;21:543-50.
  25. Konttinen YT, Lappalainen R, Laine P, Kitti U, Santavirta S, Teronen O. Immunohistochemical evaluation of inflammatory mediators in failing implants. Int J Periodontics Restorative Dent 2006;26:135-41.
  26. Mengel R, Stelzel M, Hasse C, Flores-de-Jacoby L. Osseointegrated implants in patients treated for generalized severe adult periodontitis. An interim report. J Periodontol 1996;67:782-7. https://doi.org/10.1902/jop.1996.67.8.782