Comparison of Oven-drying Methods for Determination of Moisture Content in Feed Ingredients

  • Ahn, J.Y. (Department of Animal Science and Technology, Konkuk University) ;
  • Kil, D.Y. (Department of Animal Science and Technology, Chung-Ang University) ;
  • Kong, C. (Department of Animal Science and Technology, Konkuk University) ;
  • Kim, B.G. (Department of Animal Science and Technology, Konkuk University)
  • Received : 2014.04.23
  • Accepted : 2014.07.19
  • Published : 2014.11.01


An accurate assessment of moisture content in feed ingredients is important because moisture influences the nutritional evaluation of feedstuffs. The objective of this study was to evaluate various methods for moisture content determination. In Exp. 1, the weight loss on drying (LOD) of corn, soybean meal (SBM), distillers dried grains with solubles (DDGS), whey permeate, whey powder, spray-dried porcine plasma (SDPP), fish meal, and a mixed diet of these 7 ingredients were measured by oven drying at $135^{\circ}C$ for 2 h. Additionally, the samples were dried at $105^{\circ}C$ for 3, 6, 9, 12, or 15 h. The LOD contents of the DDGS, whey permeate, and whey powder measured by drying at $135^{\circ}C$ for 2 h were greater than the values measured by drying at $105^{\circ}C$ for 3 h (p<0.05). All samples except SDPP (p = 0.70) dried at $105^{\circ}C$ for 6, 9, 12, or 15 h caused more LOD compared with the samples dried for at $105^{\circ}C$ for 3 h (p<0.05). The LOD contents of the individual ingredients were additive when dried at $105^{\circ}C$ regardless of drying time. In Exp. 2, moisture contents of corn, SBM, wheat, whey permeate, whey powder, lactose, and 2 sources of DDGS (DDGS1 and DDGS2) were measured by the Karl Fischer method, oven drying at $135^{\circ}C$ for 2 h, and oven drying at $125^{\circ}C$, $115^{\circ}C$, $105^{\circ}C$, or $95^{\circ}C$ for increasing drying time from 1 to 24 h. Drying samples at $135^{\circ}C$ for 2 h resulted in higher moisture content in whey permeate (7.5% vs 3.0%), whey powder (7.7% vs 3.8%), DDGS1 (11.4% vs 7.5%), and DDGS2 (13.1% vs 8.8%) compared with the Karl Fischer method (p<0.05). Whey permeate and whey powder were considerably darkened as the drying time increased. In conclusion, drying samples at $135^{\circ}C$ for 2 h is not appropriate for determining the moisture content in whey permeate, whey powder, or DDGS as well as the mixed diet containing these ingredients. The oven-drying method at $105^{\circ}C$ for 5 to 6 h appears to be appropriate for whey permeate and whey powder, and at $105^{\circ}C$ for 2 to 3 h for DDGS.


Supported by : Konkuk University


  1. AOAC. 2005. Official Methods of Analysis. 18th edn. Association of Official Analytical Chemists. Arlington, VA, USA.
  2. Ileleji, K. E., A. A. Garcia, A. R. P. Kingsly, and C. L. Clementson. 2010. Comparison of standard moisture loss-on-drying methods for the determination of moisture content of corn distillers dried grains with solubles. J. AOAC Int. 93:825-832.
  3. Isengard, H.-D., R. Kling, and C. T. Reh. 2006. Proposal of a new reference method to determine the water content of dried dairy products. Food Chem. 96:418-422.
  4. Kim, B. G., D. Y. Kil, Y. Zhang, and H. H. Stein. 2012. Concentrations of analyzed or reactive lysine, but not crude protein, may predict the concentration of digestible lysine in distillers dried grains with solubles fed to pigs. J. Anim. Sci. 90:3798-3808.
  5. Mo, M. and T. Tjornhom. 1978. Losses of carbon-containing substances during dry matter determination by oven drying. Acta Agric. Scand. 28:196-202.
  6. Shreve, B., N. Thiex, and M. Wolf. 2006. National Forage Testing Association Reference Method: Dry Matter by Oven Drying for 3 Hours at $100{^{\circ}C}$. NFTA Reference Methods. National Forage Testing Association, Omaha, NE, USA.
  7. Thiex, N. 2009. Evaluation of analytical methods for the determination of moisture, crude protein, crude fat, and crude fiber in distillers dried grains with solubles. J. AOAC Int. 92:61-73.
  8. Thiex, N. and C. R. Richardson. 2003. Challenges in measuring moisture content of feeds. J. Anim. Sci. 81:3255-3266.
  9. Thiex, N. and T. Van Erem. 1999. Comparisons of Karl Fischer method with oven methods for determination of water in forages and animal feeds. J. AOAC Int. 82:799-808.
  10. Windham, W. R., J. A. Robertson, and R. G. Leffler. 1987. A comparison of methods for moisture determination of forages for new infrared reflectance spectroscopy calibration and validation. Crop Sci. 27:777-783.

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