- Volume 27 Issue 11
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Effect of Lactobacillus mucosae on In vitro Rumen Fermentation Characteristics of Dried Brewers Grain, Methane Production and Bacterial Diversity
- Soriano, Alvin P. (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University) ;
- Mamuad, Lovelia L. (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University) ;
- Kim, Seon-Ho (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University) ;
- Choi, Yeon Jae (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University) ;
- Jeong, Chang Dae (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University) ;
- Bae, Gui Seck (Department of Animal Science and Technology, Chung-Ang University) ;
- Chang, Moon Baek (Department of Animal Science and Technology, Chung-Ang University) ;
- Lee, Sang Suk (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University)
- Received : 2014.07.11
- Accepted : 2014.09.14
- Published : 2014.11.01
The effects of Lactobacillus mucosae (L. mucosae), a potential direct fed microbial previously isolated from the rumen of Korean native goat, on the rumen fermentation profile of brewers grain were evaluated. Fermentation was conducted in serum bottles each containing 1% dry matter (DM) of the test substrate and either no L. mucosae (control), 1% 24 h broth culture of L. mucosae (T1), or 1% inoculation with the cell-free culture supernatant (T2). Each serum bottle was filled anaerobically with 100 mL of buffered rumen fluid and sealed prior to incubation for 0, 6, 12, 24, and 48 h from which fermentation parameters were monitored and the microbial diversity was evaluated. The results revealed that T1 had higher total gas production (65.00 mL) than the control (61.33 mL) and T2 (62.00 mL) (p<0.05) at 48 h. Consequently, T1 had significantly lower pH values (p<0.05) than the other groups at 48 h. Ammonia nitrogen (
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