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Experimental Study on Inhibition Effects of the XAF1 Gene against Lung Cancer Cell Proliferation

  • Yang, Wen-Tao (Department of Cardiothoracic Surgery, Second Affiliated Hospital, Soochow University) ;
  • Chen, Dong-Lai (Medicine Department of Soochow University) ;
  • Zhang, Fu-Quan (Department of Cardiothoracic Surgery, Second Affiliated Hospital, Soochow University) ;
  • Xia, Ying-Chen (Department of Cardiothoracic Surgery, Second Affiliated Hospital, Soochow University) ;
  • Zhu, Rong-Ying (Department of Cardiothoracic Surgery, Second Affiliated Hospital, Soochow University) ;
  • Zhou, Duan-Shan (Department of Cardiothoracic Surgery, Second Affiliated Hospital, Soochow University) ;
  • Chen, Yong-Bing (Department of Cardiothoracic Surgery, Second Affiliated Hospital, Soochow University)
  • 발행 : 2014.10.11

초록

Objective: To investigate the effect of high expression of XAF1 in vivo or in vitro on lung cancer cell growth and apoptosis. Methods: 1. The A549 human lung cancer cell line was transfected with Ad5/F35 - XAF1, or Ad5/F35 - Null at the same multiplicity of infection (MOI); (hereinafter referred to as transient transfected cell strain); XAF1 gene mRNA and protein expression was detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting respectively. 2. Methyl thiazolyl tetrazolium (MTT) and annexin V-FITC/PI double staining were used to detect cell proliferation and apoptosis before and after infection of Ad5/F35 - XAF1 with Western blotting for apoptosis related proteins, caspase 3, caspase - 8 and PARP. 3. After the XAF1 gene was transfected into lung cancer A549 cells by lentiviral vectors, and selected by screening with Blasticidin, reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were applied to detect mRNA and protein expression, to establish a line with a stable high expression of XAF1 (hereinafter referred to as stable expression cell strain). Twenty nude mice were randomly divided into groups A and B, 10 in each group: A549/XAF1 stable expression cell strain was subcutaneously injected in group A, and A549/Ctrl stable cell line stable expression cell strain in group B (control group), to observe transplanted tumor growth in nude mice. Results: The mRNA and protein expression of XAF1 in A549 cells transfected by Ad5/F35 - XAF1 was significantly higher than in the control group. XAF1 mediated by adenovirus vector demonstrated a dose dependent inhibition of lung cancer cell proliferation and induction of apoptosis. This was accompanied by cleavage of caspase -3, -8, -9 and PARP, suggesting activation of intrinsic or extrinsic apoptotic pathways. A cell strain of lung cancer highly expressing XAF1 was established, and this demonstrated delayed tumor growth after transplantation in vivo. Conclusion: Adenovirus mediated XAF1 gene expression could inhibit proliferation and induce apoptosis in lung cancer cells in vitro; highly stable expression of XAF1 could also significantly inhibit the growth of transplanted tumors in nude mouse, with no obvious adverse reactions observed. Therefore, the XAF1 gene could become a new target for lung cancer treatment.

참고문헌

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피인용 문헌

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  2. vol.299, pp.3, 2015, https://doi.org/10.1002/ar.23299
  3. Adenovirus encoding XAF-1 and TNF-α in the same open reading frame efficiently inhibits hepatocellular cancer cells vol.13, pp.6, 2016, https://doi.org/10.3892/mmr.2016.5193