DOI QR코드

DOI QR Code

Anti-Cancer Effect of Gallic Acid in CT-26 Cells Inoculated Cancer Bearing Balb/C Mice

CT-26 세포 암 유발 마우스에서 Gallic acid의 항암 효과

  • Lee, Jung Hee (Department of Beauty Science, Gwangju Women's University) ;
  • Choi, Hwa-Jung (Department of Beauty Science, Gwangju Women's University) ;
  • Kim, Pom-Ho (Department of Alternative Medicine of Graduate School of Welfare & Management, Namseoul University)
  • 이정희 (광주여자대학교 미용과학과) ;
  • 최화정 (광주여자대학교 미용과학과) ;
  • 김범호 (남서울대학교 복지경영대학원 대체치유학과)
  • Received : 2014.06.16
  • Accepted : 2014.10.10
  • Published : 2014.10.31

Abstract

This study examined the anti-cancer activity of gallic acid(GA) isolated from P. suffruticosa. was analyzed by ESI-MS, $^1H$-NMR, and $^{13}C$-NMR. The anti-cancer activity was evaluated by measuring the cancer size in CT-26 cancer-allograft mice treated with GA(100 mg/kg p.o) for 14 days. The change in body weight, acute toxicity, weight change of the liver and spleen and biomaker of the liver were evaluated in the mice after the GA treatment. As a result, the cancer size of the CT-26 cancer-allograft mice treated with GA decreased significantly compared to that of the cancer mice without significant changes in weight loss (p<0.05) and acute toxicity. The weight of the liver and spleen and ALT, AST and LPO levels increased by cancer were decreased significantly after the GA treatment, and the GSH levels decreased by cancer were increased significantly with the GA treatment (p<0.05). Therefore, GA could be an attractive lead for the development of anticancer agents.

Keywords

Anticancer agents;Biochemical markers;Gallic acid;Mice;Paeonia suffruticosa

References

  1. S. H. Park, Y. S. Cho, M. J. Kwack, H. S. Lee, C. B. Kang, "Effect of Kegel exercise on urinary incontinence after radical prostatectomy: Systematic review of randomized controlled trials," Korean Journal of Adult Nursing, Vol. 25, No. 2, pp. 219-230, 2013. DOI: http://dx.doi.org/10.7475/kjan.2013.25.2.219 https://doi.org/10.7475/kjan.2013.25.2.219
  2. J. M. Song, "Epidemiology and natural history of prostate cancer," Korean Journal of Urological Oncology, Vol. 1, No. 1, pp. 6-9, 2003.
  3. N. H. Kim, S. N. Kim, J. S. Oh, S. Lee, Y. K. Kim, "Anti-mitotic potential of 7-diethylamino-3(2′-benzoxazolyl)-coumarin in 5-fluorouracil-resistant human gastric cancer cell line SNU620/5-FU," Biochemical and Biophysical Research Communications, 2012; 418: 616-621. Vol. 25, No. 2, pp. https://doi.org/10.1016/j.bbrc.2012.01.049
  4. K. Kanetaka, A. Enjoji, J. Furui, Y. Nagata, H. Fujioka, T. Shiogama, A. Miyata, H. Kishikawa, S. Matsuo, T. Iwata, T. Kanematsu, S. Eguchi, "Effects of intermittent 5-fluorouracil and low-dose cisplatin therapy on advanced and recurrent gastric cancer," Anticancer Research, Vol. 32, No. 8, pp. 3495-3499, 2012.
  5. C. Dikken, J. Sitzia, "Patients' experiences of chemotherapy: side-effects associated with 5-fluorouracil + folinic acid in the treatment of colorectal cancer," Journal of Clinical Nursing, Vol. 7, No. 4, pp. 371-379, 1998. DOI: http://dx.doi.org/10.1046/j.1365-2702.1998.00159.x https://doi.org/10.1046/j.1365-2702.1998.00159.x
  6. H. Zhou, Y. F. Wong, X. Cai, Z. Q. Liu, Z. H. Jiang, Z. X. Bian, H. X. Xu, L Liu, "Suppressive effects of JCICM-6, the extract of an anti-arthritic herbal formula, on the experimental inflammatory and nociceptive models in rodents," Biological & Pharmaceutical Bulletin, Vol. 29, No. 2, pp. 253-260, 2006. DOI: http://dx.doi.org/10.1248/bpb.29.253 https://doi.org/10.1248/bpb.29.253
  7. S. M. Park, D. W. Jun, C. H. Park, J. S. Jang, S. K. Park, B. S. Ko, B. J. Kim, S. B. Choi, "Hyperglycemic effects of crude extracts of Moutan radicis cortex," Korean Journal of Food Science and Technology, Vol. 36, No. 3, pp. 472-477, 2004.
  8. M. Wu, Z. Gu, "Screening of bioactive compounds from Moutan cortex and their anti-inflammatory activities in rat synoviocytes," Evidence-Based Complementary and Alternative Medicine, Vol. 6, No. 1, pp. 57-63, 2009. DOI: http://dx.doi.org/10.1093/ecam/nem066 https://doi.org/10.1093/ecam/nem066
  9. O. G. Kweon, J. C. Son, S. C. Kim, S. K. Chung, S. W. Park, "Antimicrobial and antioxidative activities from Moutan cortex extract," Korean Journal of Food Preservation, Vol. 5, No. 3, pp. 281-285, 1998.
  10. H. J. Seon, H. Y. Sil, "Isolation and identification of antimicrobial compound from Mokdan bark (Paeonia suffruticosa ANDR)," Journal of the Korean Society of Food Science and Nutrition, Vol. 32, No. 7, pp. 1059-1065, 2003. DOI: http://dx.doi.org/10.3746/jkfn.2003.32.7.1059 https://doi.org/10.3746/jkfn.2003.32.7.1059
  11. S. Rho, H. S. Chung, M. Kang, E. Lee, C. Cho, H. Kim, S. Park, H. Y. Kim, M. Hong, M. Shin, H. Bae, "Inhibition of production of reactive oxygen species and gene expression profile by treatment of ethanol extract of Moutan cortex radicis in oxidative stressed PC12 cells," Biological & Pharmaceutical Bulletin, Vol. 28, No. 4, pp. 661-666, 2005. DOI: http://dx.doi.org/10.1248/bpb.28.661 https://doi.org/10.1248/bpb.28.661
  12. J. Y. Kim, H. S. Ju, J. Y. Ban, K. S. Song, Y. H. Seong, "Moutan cortex extract inhibits amyloid B protein (25-35)-induced neurotoxicity in cultured rat cortical neurons," Korean Journal of Pharmacognosy, Vol. 16, No. 6, pp. 409-415, 2008.
  13. A. Serrano, C. Palacios, G. Roy, C. Cespon, M. L. Villar, M. Nocito, "Gonzalez-Porque P. Derivatives of gallic acid induce apoptosis in tumoral cell lines and inhibit lymphocyte proliferation," Archives of Biochemistry and Biophysics, Vol. 350, No. 1, pp. 49-54, 1998. DOI: http://dx.doi.org/10.1006/abbi.1997.0474 https://doi.org/10.1006/abbi.1997.0474
  14. K. Yoshioka, T. Kataoka, T. Hayashi, M. Hasegawa, Y. Ishi, H. Hibasami, "Induction of apoptosis by gallic acid in human stomach cancer KATO III and colon adenocarcinoma COLO 205 cell lines," Oncology Reports, Vol. 7, No. 6, pp. 1221-1223, 2000.
  15. M. Salucci, L. A. Stivala, G. Maiani, R. Bugianesi, V. Vannini, "Flavonoids uptake and their effect on cell cycle of human colon adenocarcinoma cells (Caco2)," British Journal of Cancer, Vol. 86, No. 10, pp. 1645-1651, 2002. DOI: http://dx.doi.org/10.1038/sj.bjc.6600295 https://doi.org/10.1038/sj.bjc.6600295
  16. S. R. Aravind, M. M. Joseph, S. Varghese, P. Balaram, T. T. Sreelekha, "Antitumor and immunopotentiating activity of polysaccharide PST001 isolated from the seed kernel of Tamarindus indica: an in vivo study in mice," The Scientific World Journal, Vol. 2012, No. 2, pp. 361-382, 2012.
  17. K. Miquel, A. Pradines, G. Favre, "Farnesol and geranylgeraniol induce actin cytoskeleton disorganization and apoptosis in A549 lung adenocarcinoma cells," Biochemical and Biophysicla Research Communnications, Vol. 225, No. 3, pp. 869-876, 1996. DOI: http://dx.doi.org/10.1006/bbrc.1996.1265 https://doi.org/10.1006/bbrc.1996.1265
  18. M. Ishihara, H. Sakagami, "Application of semiempirical method to estimate the cytotoxic activity of gallic acid and its related compounds," Anticancer Research, Vol. 23, No. 3(B), pp. 2549-2552, 2003.
  19. G. Alessandria, S. Filippeschi, P. Sinibaldi, F. Mornet, P. Passera, F. Spreafico, P. M. Cappa, P. M. Gullino, "Influence of gangliosides on primary and metastatic neoplastic growth in human and murine cells, "Cancer Research, Vol. 47, No. 16, pp. 4243-4247, 1987.
  20. H. Ohkawa, N. Ohishi, K. Yagi, "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction," Analytical Biochemistry, Vol. 95, No. 2, pp. 351-35, 1979. DOI: http://dx.doi.org/10.1016/0003-2697(79)90738-3 https://doi.org/10.1016/0003-2697(79)90738-3
  21. O. W. Griffith, "Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinyl pridine," Analytical Biochemistry, Vol. 106, No. 1, pp. 207-212, 1980. DOI: http://dx.doi.org/10.1016/0003-2697(80)90139-6 https://doi.org/10.1016/0003-2697(80)90139-6
  22. S. Reitman, S. K. Frankel, "A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase," American Journal of Clinical Pathology, Vol. 28, No. 1, pp. 56-63, 1957. https://doi.org/10.1093/ajcp/28.1.56
  23. O. Benavente-Garcia, J. Castillo, F. R. Marin, A. Ortuno, J. A. Del Rio, "Uses and properties of citrus flavonoids," Journal of the Agricultural and Food Chemistry, Vol. 45, No. 12, pp. 4505-4515, 1997. DOI: http://dx.doi.org/10.1021/jf970373s https://doi.org/10.1021/jf970373s
  24. G. Galati, P. J. O'Brien, "Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties," Free Radical Biology and Medicine, Vol. 37, No. 3, pp. 287-303, 2004. DOI: http://dx.doi.org/10.1016/j.freeradbiomed.2004.04.034 https://doi.org/10.1016/j.freeradbiomed.2004.04.034
  25. G. S. Oh, H. O. Pae, B. M. Choi, H. S. Lee, I. K. Kim, Y. G. Yun, J. D. Kim, H. T. Chung, "Penta-O-galloylbeta- D-glucose inhibits phorbol myristate acetate-induced interleukin-8 [correction of intereukin-8] gene expression in human monocytic U937 cells through its inactivation of nuclear factor-kappa B," International Immunopharmacology, Vol. 4, No. 3, pp. 377-386, 2004. DOI: http://dx.doi.org/10.1016/j.intimp.2003.10.010 https://doi.org/10.1016/j.intimp.2003.10.010
  26. J. Kim, H. Lee, Y. Lee, B. G. Oh, C. Cho, Y. Kim, M. Shin, M. Hong, S. K. Jung, H. Bae, "Inhibition effects of Moutan Cortex Radicis on secrtion of eotaxin in A549 human epithelial cells and eosinophil migration," Journal of Ethnopharmacology, Vol. 114, No. 2, pp. 186-193, 2007. DOI: http://dx.doi.org/10.1016/j.jep.2007.07.038 https://doi.org/10.1016/j.jep.2007.07.038
  27. R. M. Breikaa, M. M. Algandaby, E. El-Demerdash, A. B. Abdel-Naim, "Biochanin A protects against acute carbon tetrachloride-Induced hepatotoxicity in rats," Bioscience, Biotechnology, and Biochemistry, Vol. 77, No. 5, pp. 909-916, 2013. DOI: http://dx.doi.org/10.1271/bbb.120675 https://doi.org/10.1271/bbb.120675
  28. C. Aydin, Y. Ay, H. Basel, S. .Kavak, B. Inan, H. Bektas, H. A. Gümrükçüoğlu, H. Ekim, H. Demir, "Analysis of the influences of shortterm levosimendan exposure on oxidant/antioxidant status and trace-element levels in the physiological status of the thoracic aorta of rats," The Journal of Membrane Biology, Vol. 245, No. 12, pp. 827-832, 2012. https://doi.org/10.1007/s00232-012-9489-4
  29. J. R. Mitchell, D. J. Jollow, W. Z. Potter, D. C Davis, J. R. Gillette, B. B. Brodie, "Acetaminophen-induced hepatic necrosis. I. Role of drug metabolism," The Journal of Pharmacology and Experimental Therapeutics,. Vol. 187, No. 1, pp. 185-194, 1973.
  30. J. Eliza, P. Daisy, S. Ignacimuthu, V. Duraipandiyan, "Antidiabetic and antilipidemic effect of eremanthin from Costus speciosus (Koen.) Sm., in STZ-induced diabetic rats," Chemico-Biological Interactions, 2009; 182:. Vol. 182, No. 1, pp. 67-72, 2009. https://doi.org/10.1016/j.cbi.2009.08.012