Effects of Carnosic Acid on Muscle Growth in Zebrafish (Danio rerio)

제브라피쉬 근육성장에서의 carnosic acid의 효과

  • Kim, Jeong Hwan (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University) ;
  • Jin, Deuk-Hee (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University) ;
  • Kim, Young-Dae (Aquaculture Division, East Sea Regional Fisheries Research Institute) ;
  • Jin, Hyung-Joo (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University)
  • 김정환 (강릉원주대학교 해양분자생명공학과) ;
  • 진덕희 (강릉원주대학교 해양분자생명공학과) ;
  • 김영대 (국립수산과학원 동해수산연구소) ;
  • 진형주 (강릉원주대학교 해양분자생명공학과)
  • Received : 2014.06.16
  • Accepted : 2014.07.30
  • Published : 2014.09.30

Abstract

Myogenesis is the formation process of multinucleated myofiber with a contractile capacity from muscle satellite cell (MSCs) during life. This process is tightly controlled by several transcription factors such as Pax3 and Pax7 (paired box protein 3 and 7), MEF2C (myocyte enhancer factor 2) and MRFs (myogenic regulatory factors) etc. On the contrary, myostatin (MSTN) is a transforming growth factor-${\beta}$ superfamily, which functions as a negative regulator of skeletal muscle development and growth. Carnosic acid (CA) is a major phenolic component in rosemary (Rosmarinus officinalis) and have been reported various biological activities such as anticancer, antioxidant, antimicrobial and therapeutic agents for amnesia, dementia, alzheimer's disease. This study was confirmed to effects of CA on muscle cell line and muscle tissue alteration of zebrafish by intramuscular injection or feeding methods. $10{\mu}M$ CA showed a non-cytotoxic on myoblast and a complete inhibition effect against myostatin activity on luciferase assay. In intramuscular injection experiment, the total protein and triglyceride amount of $10{\mu}M/kg$ of CA injected group increased by 11% and decreased by 13% compared to these of the no injected group. In histology analysis of muscle tissues by hematoxylin/eosin staining, the number of muscle fiber of $10{\mu}M/kg$ of CA injected group decreased by 29% and fiber area increased 40% compared to these of no injected group. In feeding experiment, the total protein and triglyceride amount no significance difference compared to these of the normal feeding group. In histology analysis, the number of muscle fiber of 1% CA fed group decreased by 35% and fiber area increased 56% compared to these of normal fed group. We identified that CA have an effect on hypertrophy of muscle fiber in adult zebrafish and the results of this study are considered as the basic data that can reveal the mechanisms of muscle formation via gene and protein level analysis.

Acknowledgement

Supported by : 강릉원주대학교

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