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Effects of Low-Dose Fractionated Total Body Irradiation on Murine Immune System

마우스에서 전신 저선량 분할 방사선 조사에 의한 면역학적 변화 평가

  • Kim, Mi-Hyoung (Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences) ;
  • Rhu, Sang-Young (Departments of Obstetrics and Gynecology, Korea Cancer Canter Hospital, Korea Institute of Radiological & Medical Sciences) ;
  • Lim, Dae-Seog (Department of Biotechnology, CHA university) ;
  • Song, Jie-Young (Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences)
  • 김미형 (한국원자력의학원 방사선의학연구소 방사선암연구부) ;
  • 유상영 (한국원자력의학원 원자력병원 산부의과) ;
  • 임대석 (차의과학대학교 생명과학대학 바이오공학과) ;
  • 송지영 (한국원자력의학원 방사선의학연구소 방사선암연구부)
  • Received : 2014.07.08
  • Accepted : 2014.08.10
  • Published : 2014.09.30

Abstract

Along with the wide use of radiotherapy in cancer treatment, there is growing interest in beneficial effect of low-dose irradiation (LDI) in cancer therapy. Therefore, we investigate how LDI affects immune responses in mice model. Total body irradiation (TBI) on C57BL/6 mice was given at low-dose rate of $1mGy{\cdot}min^{-1}$ using $^{137}Cs$ source at three times for consecutive three days. Hematological examination, total cell numbers of spleen, populations and characteristics of splenocytes were determined. Total numbers of RBC or platelet in irradiated mice showed no significant changes. WBC counts were decreased in a dose-dependent manner 2 days after TBI, however, these differences are gradually waned until 28 days. Dose-dependent decrease in the number of splenocytes of TBI mice at day 2 was also improved as time progressed. While the level of Foxp3 mRNA was decreased, the frequency of $CD4^+$ T cells and $CD69^+$ cells in spleen was increased at day 2 and 14. Fractionated low-dose TBI on mice exhibited normal body weight with no distinguishable behavior during whole experimental periods. These results suggest that some parameters of immune system could be altered and evaluated by fractionated low-dose TBI and be used to broaden boundary of low dose radiation research.

Acknowledgement

Supported by : 지식경제부

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