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The activity of ${\alpha}$-amylase and ${\alpha}$-glucosidase as anti-diabetic function

  • Kim, Misook (Department of Food Science and Nutrition, Dankook University) ;
  • Kim, Eunji (Department of Food Science and Nutrition, Dankook University) ;
  • Kwak, Han Sub (Department of Food Science and Nutrition, Dankook University) ;
  • Jeong, Yoonhwa (Department of Food Science and Nutrition, Dankook University)
  • Received : 2013.12.31
  • Accepted : 2014.01.27
  • Published : 2014.10.01

Abstract

BACKGROUND/OBJECTIVES: We investigated total 26 ingredients of Saengshik which will be commercially produced as an anti-diabetic dietary supplement. SUBJECTS/METHODS: Thirteen vegetables, nine cereals, three legumes and one seed were extracted with aqueous ethanol for 2 h at $60^{\circ}C$, and evaluated for their inhibitory effects against ${\alpha}$-amylase and ${\alpha}$-glucosidase and for total phenolic and flavonoid contents. RESULTS: All ingredients inhibited ${\alpha}$-amylase activity except cabbage. Strong inhibitory activity of ${\alpha}$-amylase was observed in leek, black rice, angelica and barley compared with acarbose as a positive control. Stronger inhibition of ${\alpha}$-glucosidase activity was found in small water dropwort, radish leaves, sorghum and cabbage than acarbose. All Saengshik ingredients suppressed ${\alpha}$-glucosidase activity in the range of 0.3-60.5%. Most ingredients contained total phenols which were in the range of 1.2-229.4 mg gallic acid equivalent/g dried extract. But, total phenolic contents were not observed in carrot, pumpkin and radish. All ingredients contained flavonoid in the range of 11.6-380.7 mg catechin equivalent/g dried extract. CONCLUSIONS: Our results demonstrate that Saengshik containing these ingredients would be an effective dietary supplement for diabetes.

Keywords

Saengshik;${\alpha}$-amylase inhibition;${\alpha}$-glucosidase inhibition;total phenolic compounds;antioxidant activity

Acknowledgement

Supported by : Ministry of Agriculture, Food and Rural Affairs

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