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Apoptotic Effects of psiRNA-STAT3 on 4T1 Breast Cancer Cells in Vitro

  • Zhou, Yue (School of Pharmacy, The Second Clinical Hospital) ;
  • Tian, Lin (School of Pharmacy, The Second Clinical Hospital) ;
  • Zhang, Ying-Chao (Department of Breast Surgery, The Second Clinical Hospital) ;
  • Guo, Bao-Feng (Department of Plastic Surgery, the China-Japan Union Hospital) ;
  • Zhou, Qing-Wei (Department of Biology and Medical Engineering, Institute of Regenerative Medicine, Jilin University)
  • Published : 2014.08.30

Abstract

Background: The aim of this study was to investigate the effect of a Lipofectamine2000 (Life2000) Transfection Reagent transfected psiRNA-STAT3 plasmid on 4T1 breast cancer cells. Materials and Methods: MTT was used to detect the cell proliferation of breast cancer 4T1 cells at different periods (0h, 6h, 8h, 10h); the cell cycle was assessed by flow cytometry; variation of apoptosis and mitochondrial membrane potential was observed under a fluorescence microscope; immunohistochemical staining was used to determine the expression of caspase-3 and cyclin-D1 protein. Results: An obvious effect of inhibition to 4T1 cancer cells could be observed at 8h after the psiRNA-STAT3 was transfected. Typical alterations of apoptotic morphological features were visible in the psiRNA-STAT3 treatment group. Mitochondrial membrane potential decreased significantly, the number of cells was increased in G0/G1 phase, and the number of cells was decreased in S phase, and the data were statistically significant (p<0.05), compared with the Scramble and Mock groups. Expression of caspase-3 protein was increased significantly, while that of cyclin D1 was significantly decreased. Conclusions: Life2000 transfected psiRNA-STAT3 plasmid can inhibit 4T1 tumor cell proliferation and promote apoptosis of 4T1 tumor cells, which process depends on the regulation of expression of cyclin D1 and caspase-3 protein.

Keywords

psiRNA-STAT3 plasmid;breast cancer 4T1 cells;apoptosis;protein expression

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