Establishment and Partial Characterization of an Epirubicin-Resistant Gastric Cancer Cell Line with Upregulated ABCB1

  • Felipe, Aledson Vitor (Department of Medicine, Gastroenterology Division, Federal University) ;
  • Moraes, Andrea Aparecida (Department of Medicine, Gastroenterology Division, Federal University) ;
  • de Oliveira, Juliana (Department of Medicine, Gastroenterology Division, Federal University) ;
  • da Silva, Tiago Donizetti (Department of Medicine, Gastroenterology Division, Federal University) ;
  • Forones, Nora Manoukian (Department of Medicine, Gastroenterology Division, Federal University)
  • Published : 2014.08.30


Multidrug resistance (MDR) is a major impediment to successful chemotherapy of gastric cancer. Our aim was to establish an epirubicin-resistant cell subline (AGS/EPI) and to elucidate the mechanisms involved in acquired EPI resistance. The AGS/EPI cell subline developed by exposing parental AGS cells to stepwise increasing concentrations of EPI demonstrated 2.52-fold resistance relative to the AGS cell line, and mRNA expression of the ATP-dependent drug-efflux pump P-glycoprotein (Pgp), more recently known as ABCB1 protein, was similarly upregulated. An AGS/EPI cell subline could thus be effectively established, and MDR mechanism of these cells was shown to be related to the overexpression of mRNA of the ABCB1 gene.


Multidrug resistance;gastric cancer;ABCB1;epirubicin


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