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Expression of Cyclooxygenase-2 (COX-2) in Colorectal Adenocarcinoma: an Immunohistochemical and Histopathological Study

  • Mahmoud, Abla Sayed (Department of Oral Maxillofacial Sciences, Alfarabi College of Dentistry) ;
  • Umair, Ayesha (Department of Oral Maxillofacial Sciences, Alfarabi College of Nursing and Dentistry) ;
  • Azzeghaiby, Saleh Nasser (Department of Oral Maxillofacial Sciences, Alfarabi College of Nursing and Dentistry) ;
  • Alqahtani, Fahad Hussain (Department of Oral Maxillofacial Sciences, Alfarabi College of Nursing and Dentistry) ;
  • Hanouneh, Salah (Department of Oral Maxillofacial Sciences, Alfarabi College of Nursing and Dentistry) ;
  • Tarakji, Bassel (Department of Oral Maxillofacial Sciences, Alfarabi College of Nursing and Dentistry)
  • Published : 2014.08.30

Abstract

Background: The aim of this study was to evaluate cyclooxygenase-2 (COX-2) immunoreactivity in colorectal adenocarcinomas and to find correlations with different pathological features. Materials and Methods: This study included 35 cases of colorectal carcinoma foir which surgical colectomy specimens were collected. Immunohistochemical staining of COX-2 (cyclooxygenase-2) is done by using the Streptavidin-biotin technique. Results: This work reveals that COX-2 is positive in most cases of colorectal carcinoma and negative in normal colon tissue with statistically non significant relations between COX-2 immunostaining and different pathological features. Conclusions: Our data suggest over expression of COX-2 protein in colorectal carcinoma in contrast to normal mucosa, with a possible role in cell proliferation in carcinogenesis.

Keywords

COX-2;antibodies;colorectal adenocarcinoma;immunohistochemistry

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