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C13orf18 and C1orf166 (MULAN) DNA Genes Methylation are Not Associated with Cervical Cancer and Precancerous Lesions of Human Papillomavirus Genotypes in Iranian Women

  • Sohrabi, Amir (Department of Molecular Medicine, School of Advanced Medical Technologies, Tehran University of Medical Sciences) ;
  • Mirab-Samiee, Siamak (Food and Drug Laboratory Research Center, Ministry of Health and Medical Education) ;
  • Rahnamaye-Farzami, Marjan (Research Center of Health Reference Laboratory, Ministry of Health and Medical Education) ;
  • Rafizadeh, Mitra (Department of Pathology, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences) ;
  • Akhavan, Setareh (Department of Gynecology, Vali-e-asr Hospital, Tehran University of Medical Sciences) ;
  • Hashemi-Bahremani, Mohammad (Department of Pathology, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences) ;
  • Modarressi, Mohammad Hossein (Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences)
  • Published : 2014.08.30

Abstract

Background: Nowadays, molecular biomarkers have critical roles for cancer diagnosis and prognosis in clinical laboratories. Human papillomaviruses are the main agents for etiology of cervical carcinoma. The present survey was conducted to evaluate the genes methylation in cervical cancer and precancerous lesions involvement with HPV genotypes. Materials and Methods: C13orf18 and C10rf166 (MULl or Mulan) DNA methylation as potential biomarkers and risk factors was investigated in 112 liquid based cytology and Formalin-Fixed Paraffin-Embedded tissue specimens in Iranian females with cervical intraepithelial neoplasia and dysplasia. Results: In this survey, HPV18 (61.6%) and HPV16 (42.9%) proved to be the most common HPV genotypes identified by In-House Multiplex Real Time PCR. There were no significant relationship between HPV positivity and the methylated DNA genes mentioned above (p>0.05). Conclusions: Our MethyLight data demonstrated that these genes could not be considered as specific, sensitive and suitable prognostic biomarkers in cervical dysplasia related HPV. It is suggested that further studies with more patients should be done on candidate methylated markers in different countries in order to plan for cervical cancer prevention.

Keywords

HPV;methylation;C13orf18;C1orf166;cervical neoplasia;Iran

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