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Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cell Line

  • Raj, Kathamuthu Gokul (Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University) ;
  • Sambantham, Shanmugam (Department of Genetics, Dr. ALMPGIBMS, University of Madras) ;
  • Manikanadan, Ramar (Department of Zoology, University of Madras) ;
  • Arulvasu, Chinnansamy (Department of Zoology, University of Madras) ;
  • Pandi, Mohan (Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University)
  • Published : 2014.08.30

Abstract

Purpose: The present study concerns molecular mechanisms involved in induction of apoptosis by a fungal taxol extracted from the fungus Cladosporium oxysporum in T47D human breast cancer cells. Materials and Methods: Apoptosis-induced by the fungal taxol was assessed by MTT assay, nuclear staining, DNA fragmentation, flow cytometry and pro- as well as anti-apoptotic protein expression by Western blotting. Results: Our results showed inhibition of T47D cell proliferation with an $IC_{50}$ value of $2.5{\mu}M/ml$ after 24 h incubation. It was suggested that the extract may exert its anti-proliferative effect on human breast cancer cell line by suppressing growth, arresting through the cell cycle, increase in DNA fragmentation as well as down-regulation of the expression of NF-${\kappa}B$, Bcl-2 and Bcl-XL and up-regulation of pro-apoptotic proteins like Bax, cyt-C and caspase-3. Conclusions: We propose that the fungal taxol contributes to growth inhibition in the human breast cancer cell through apoptosis induction via a mitochondrial mediated pathway, with possible potential as an anticancer therapeutic agent.

Keywords

Fungal taxol;apoptosis;DNA fragmentation;DNA damage;NF-${\kappa}B$

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