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Mangiferin Induces Apoptosis by Regulating Bcl-2 and Bax Expression in the CNE2 Nasopharyngeal Carcinoma Cell Line

  • Pan, Li-Li (Department of Biochemistry and Molecular Biology, Guangxi Medical University) ;
  • Wang, Ai-Yan (Department of Biochemistry and Molecular Biology, Guangxi Medical University) ;
  • Huang, Yong-Qi (Department of Biochemistry and Molecular Biology, Guangxi Medical University) ;
  • Luo, Yu (Department of Biochemistry and Molecular Biology, Guangxi Medical University) ;
  • Ling, Min (Department of Biochemistry and Molecular Biology, Guangxi Medical University)
  • Published : 2014.09.15

Abstract

To investigate the anti-proliferative mechanism of mangiferin in a human nasopharyngeal carcinoma cell line, CNE2 cells were incubated with different concentrations of mangiferin (12.5, 25, 50, 100, 150 and $200{\mu}M$) or with PBS as a control for 72 hours. Analyses were made of the cell cycle and apoptosis with measurement of mRNA and protein levels of two apoptosis-related genes, Bcl-2 and Bax. Flow cytometry assays showed mangiferin could inhibit CNE2 cell proliferation via G2/M arrest and induction of early apoptosis. Real time PCR and Western blotting showed the mRNA and protein level of Bcl-2 to be down-regulated, while those of Bax were upregulated, when CNE2 cells were treated with mangiferin. This investigation indicated anti-proliferation effects of mangiferin through induction of cell apoptosis regulated by Bcl-2 and Bax expression.

Keywords

Mangiferin;CNE2;apoptosis;Bcl-2;Bax

Acknowledgement

Supported by : Guangxi Colleges and Universities

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