- Volume 30 Issue 3
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First Report of Bacterial Wilt Caused by Ralstonia solanacearum Biovar 2 Race 1 on Tomato in Egypt
- Seleim, Mohamed A.A. (Plant Pathology Department, Faculty of Agriculture, Al-Azhar University) ;
- Abo-Elyousr, Kamal A.M. (Plant Pathology Department, Faculty of Agriculture, Assiut University) ;
- Abd-El-Moneem, Kenawy M. (Plant Pathology Department, Faculty of Agriculture, Assiut University) ;
- Saead, Farag A. (Plant Pathology Department, Faculty of Agriculture, Assiut University)
- Received : 2013.11.02
- Accepted : 2014.04.07
- Published : 2014.09.01
This study aims to isolate and identify the causal pathogen of tomato bacterial wilt in Egypt. In 2008, tomato plants showing typical symptoms of bacterial wilt disease with no foliar yellowing were observed in Minia, Assiut and Sohag governorates, Egypt. When cut stems of symptomatic plants were submerged in water, whitish ooze was evident and longitudinal sections showed a brown discoloration in the vascular tissues. Bacteria were isolated on triphenyl tetrazolium chloride medium and fifteen isolates shown typical morphological and cultural characteristics were confirmed as Ralstonia solanacearum biovar 2 race 1. Pathogenicity tests showed that all isolates proved to be pathogenic to tomato plants, varied from 52 to 97% wilting. This is the first report of R. solanacearum biovar 2 race 1 causing bacterial wilt in tomato crop in Egypt.
- Abo-Elyousr, K. A. and Asran, M. R. 2009. Antibacterial activity of certain plant extracts against bacterial wilt of tomato. Arch. Phytopathol. Plant Protect. 42:573-578. https://doi.org/10.1080/03235400701284740
- Anon. 2004. Ralstonia solanacearum. EPPO Bulletin 34:173-178. https://doi.org/10.1111/j.1365-2338.2004.00715.x
- Araud-Razou, I., Vasse, J., Montrozier, H., Etchebar, C. and Trigalet, A. 1998. Detection and visualization of the major acidic exopolysaccharide of Ralstonia solanacearum and its role in tomato root infection and vascular colonization. Eur. J. Plant Pathol. 104:795-809. https://doi.org/10.1023/A:1008690712318
- Billing, E., Baker, L. A. E., Crosse, J. E. and Garcett, C. M. E. 1961. Laboratory diagnosis of fire blight and bacterial blossom blight of pear. Plant Pathol. 60:19-25.
- Buddenhagen, I. W., Sequeira, L. and Kelman, A. 1962. Designation of races of Pseudomonas solanacearum. Phytopathology 52:726.
- Denny, T. P. and Hayward, A. C. 2001. Ralstonia. In N. W. Schaad, J. B. Jones, & W. Chun (Eds.), Laboratory Guide for Identification of Plant Pathogenic Bacteria, 3rd Ed. (pp. 165- 189). St. Paul, MN. APS Press.
- Dye, D. W. 1968. A taxonomic study of the genus Erwinia. I. the "amylovora" group. New Zeal. J. Sci. 11: 590-607.
- El-Ariqi, S. N. S., El-Moflehi, M., El-Arbara, K., El-Kobati, A. and El-Shaari, A. 2005. Antibacterial activity of extracts from Withania somnifera and Aloe vera aginst Ralstonia solanacearum in potato. Arab J. Pl. Prot. 23:95-99.
- Elphinstone, J. G., Hennessy, J., Wilson, J. K. and Stead, D. E. 1996. Sensitivity of different methods for the detection of Pseudomonas solanacearum in potato tuber extracts. EPPO/OEPP Bulletin 26:663-678. https://doi.org/10.1111/j.1365-2338.1996.tb01511.x
- Food and Agriculture Organization of United Nations, Agriculture data FAO. 2010. htpp://www.foa.org .
- Galal, Y. G. M., El-ghandour, I. A., Osman, M. E. and Abdel- Raouf, A. M. N. 2003. The effect of inoculation by mycorrhizae and Rhizobium on the growth and yield of wheat in relation to nitrogen and phosphorus fertilization as assessed by 15N techniques. Symbiosis 34:171-183.
- Gartemann, K. H., Kirchner, O., Engemann, J., Grafen, I., Eichenlaub, R. and Burger, A. 2003. Clavibacter michiganensis subsp. michiganensis: First steps in the understanding of virulence of a Gram-positive phytopathogenic bacterium. J. Biotechnol. 106:179-191. https://doi.org/10.1016/j.jbiotec.2003.07.011
- Granada, G. A. and Sequeira, L. 1975. A hypersensitive reaction induced in tobacco leaves by a compatible isolate of Pseudomonas solanacearum. Phytopathology 65:731-733. https://doi.org/10.1094/Phyto-65-731
- Hayward, A. C. 1964. Characteristics of Pseudomonas solanacearum. J. Appl. Bacteriol. 27:265-277. https://doi.org/10.1111/j.1365-2672.1964.tb04912.x
- Hayward, A. C. 1994. Systematics and phylogeny of Pseudomonas solanacearum and related bacteria. In A. C. Hayward & G. L. Hartman (Eds.), Bacterial Wilt: The Disease and its Causative Agent,Pseudomonas solanacearum (pp. 123-135). Wallingford, UK: CAB International.
- Holt, J. G., Krieg, N. R., Sneathm, P. H. A., Staley, J. T. and Williams, S. T. 1994. Bergey's Manual of Determinative Bacteriology 9th Eds Williams and Wilkinc, Baltimore, MD, USA. 787 pp.
- Ji, P., Momol M. T., Olson, S. M., Pradhanang, P . M. and Jones, J. B. 2005. Evolution of Thymol as Biofunmigant for control of Bacterial wilt of tomato under field conditions. Plant Dis. 89: 497-500. https://doi.org/10.1094/PD-89-0497
- Kelman, A. 1954. The relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium medium. Phytopathology 44:693-695.
- Kishun, R. 1985. Effect of bacterial wilt on yield of tomato. Indian Phytopathol. 38:606.
- Klement, Z. 1990. Tobacco (HR) test for the quick demonstration of pathogenicity. In: Methods in Phytobacteriology. Klement, Z., Rudolph, K. and Sands, D. C., eds. Akademiai Kiado, Budapest. pp. 101-102.
- Krieg, N. R. and Holt, J. G. 1984. Bergey's Manual of Systematic Bacteriology, Williams and Wilkins, Baltimore and London.
- McGarvey, J. A., Denny, T. P. and Schell, M. A. 1999. Spatialtemporal and quantitative analysis of growth and EPS I production by Ralstonia solanacearum in resistant and susceptible tomato cultivars. Phytopathology 89:1233-1239. https://doi.org/10.1094/PHYTO.1918.104.22.1683
- Poussier, S., Cheron, J. J., Couteau, A. and Luisetti, J. 2002. Evaluation of procedures for reliable PCR detection of Ralstonia solanacearum in common natural substrates. J. Microbiol. Methods 51:349-359. https://doi.org/10.1016/S0167-7012(02)00111-2
- Pradhanang, P. M., Elphinstone, J. G. and Fox, R. T. V. 2000. Sensitive detection of Ralstonia solanacearum in soil: a comparison of different detection techniques. Plant Pathol. 49: 414-422. https://doi.org/10.1046/j.1365-3059.2000.00481.x
- Robertson, A. E., Wechter, W. P., Denny, T. P., Fortnum, B. A. and Kluepfel, D. A. 2004. Relationship between avirulence gene (avrA) diversity in Ralstonia solanacearum and bacterial wilt incidence. Mol. Plant-Microbe Interact. 17:1376-1384. https://doi.org/10.1094/MPMI.2004.17.12.1376
- Saile, E., Schell, M. A. and Denny, T. P. 1997. Role of extracellular polysaccharide and endoglucanase inroot invasion and colonization of tomato plants by Ralstonia solanacearum. Phytopathology 87:1264-1271. https://doi.org/10.1094/PHYTO.1922.214.171.1244
- Schaad, N. W. 1992. Laboratory Guide for Identification of Plant Pathogenic Bacteria, 2nd Edn. International Book Distributing Co., Lucknow, pp 44-58.
- Tahat, M. M. and Kamaruzaman, S. 2010. Ralstonia solanacearum: The Bacterial Wilt Causal Agent. Asian J. Plant Sci. 9:385-393. https://doi.org/10.3923/ajps.2010.385.393
- Van der Wolf, J. M., Vriend, S. G. C., Kastelein, P., Nijhuis, E. H., van Bekkum, P. J. and van Vuurde, J. W. L. 2000. Immunofluorescence colony-staining (IFC) for detection and quantification of Ralstonia (Pseudomonas) solanacearum biovar 2 (race 3) in soil and verification of positive results by PCR and dilution plating. Eur. J. Plant Pathol. 106:123-133. https://doi.org/10.1023/A:1008728828144
- Williamson, L., Nakaho, K., Hudelson, B. and Allen, C. 2002. Ralstonia solanacearum race 3, biovar 2 strains isolated from geranium are pathogenic on potato. Plant Dis. 86:987-991. https://doi.org/10.1094/PDIS.2002.86.9.987
- Winstead, N. N. and Kelman, A. 1952. Inoculation techniques for evaluating resistance to Pseudomonas solanacearum. Phytopathology 42:628-634.
- Identification and analysis of differentially expressed genes in Solanum lines in response to challenge with a Ralstonia solanacearum phylotype I strain vol.8, 2016, https://doi.org/10.1016/j.plgene.2016.09.002
- Ralstonia solanacearum upregulates marker genes of the salicylic acid and ethylene signaling pathways but not those of the jasmonic acid pathway in leaflets of Solanum lines during early stage of infection vol.147, pp.3, 2017, https://doi.org/10.1007/s10658-016-1030-7
- Species Complex: A Quick Diagnostic Guide pp.1535-1025, 2019, https://doi.org/10.1094/PHP-04-18-0015-DG