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Enhancing Effect of Pleurotus ostreatus Extracts on Mouse Spleen and Cytokine Cells Activation

느타리버섯 물 추출물 투여에 의한 마우스 비장세포 및 사이토카인 활성효과

  • Received : 2014.05.30
  • Accepted : 2014.08.07
  • Published : 2014.08.30

Abstract

Pleurotus ostreatus have been used as a traditional remedy and food source. Its anti-inflammatory, anti-oxidation effects have been previously reported. The production of cytokines (IL-$2{\beta}$, IFN-${\gamma}$, and TNF-${\alpha}$) secreted by LPS- and non LPS-stimulated macrophages, were detected by ELISA assay using a cytokine kit. Mouse splenocytes proliferation increased with Pleurotus ostreatus water extracts supplement at 5, 10, 50, 100, 250, 500, $1,000{\mu}g/mL$ after a 48hr pre-treatment with the mitogen (ConA or LPS). The cytokine production (IL-$2{\beta}$, IFN-${\gamma}$, and TNF-${\alpha}$), measured by a cytokine ELISA kit, increased on water extracts supplementation. This in vitro study suggested that supplementation with Pleurotus ostreatus water extracts may enhance the immune function by regulating the splenocyte proliferation and enhancing cytokine production.

Keywords

splenocytes proliferation;Pleurotus ostreatus;cytokine;immune

References

  1. Brubaker JO, Thompson CM, Morrison LA, Knipe DM, Siber, GR, Finberg RW. 1996. Th1-associated immune responses to beta-galactosidase expressed by replication-defective herpes simplex virus. J Immunol 157:1598-1604
  2. Barnes PJ, Liew FY. 1995 Nitric oxide and asthmatic inflammation. Immunology Today 16:128-130 https://doi.org/10.1016/0167-5699(95)80128-6
  3. Chao CC, Hu S, Molitor TW, Brosnan CF, Berman JW. 1998. Cytokine prodution by human fetal microglia and astrocytes. J Immunol 150:2659-2660
  4. Cho SH, Lee SD, Ryu JS, Kim NG, Lee DS. 2001. Change in quality of king oyster mushroom during modified atmosphere storage. Korean J Postharvest Sci Technol 8:367-373
  5. Han SY, Park MS, Seo KI. 2002. Biological activities of ostreatus mushroom kimchi. Korean J Food Sci & Technol 30:1373-1380
  6. Jung IC, Park S, Park KS, Ha HC, Kim SH, Kwon YI, Lee JS. 1996. Antioxidative effect of fruit body and mycelial extracts of Pleurotus ostreatus. Kor J Food Science & Technology 28:464-469
  7. Kang TS, Kang MS, Sung JM, Kang AS, Shon HR, Lee SY. 2001. Effect of Pleurotus ostreatus on the blood glucose and cholesterol in rats. Kor J Mycology 29:86-90
  8. Ahn MR, Jeong DY, Hong SP, Song GS, Kim YS. 2003. Quality of traditional kochujang supplemented with mushrooms. J Korean Society of Agricultural Chemistry and Biotechnology 46:229-234
  9. Balkwill FR, Maylor MS, Malik S. 1990. Tumor necrosis factor as an ant cancer agent. Eur J Cance 26:641-644 https://doi.org/10.1016/0277-5379(90)90097-D
  10. Kim YS, Lee BE, Kim KJ, Lee YT, Cho KB, Chung YC. 1998. Antitumor and immunomudulatory activities of the P. grandiflorum cultivated for more than 20 years. Yakhak Hoeji 42:382-387
  11. Kang, MS, Kang TS, Kang AS, Shon HR, Sung JM. 2000. Studies on mycelial growth and artificial cultivation of Pleurotus eryngii. Kor J Mycol 28:73-80
  12. Kim HP, Son KH, Chang HW, Kang SS. 2004. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacological Sci 96:229-245 https://doi.org/10.1254/jphs.CRJ04003X
  13. Kim HP, Son KH, Kang SS. 2004. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacological Sci 96:229-245 https://doi.org/10.1254/jphs.CRJ04003X
  14. Kim YS. 1998. Quality of wet noodle prepared with wheat flour and mushroom power. Kor J Food Science & Tochnology 30:1373-1380
  15. Lee HM, Chang UJ. 2001. Effect of corn peptide on the lipid metabolism in rat. Korean J Dietary Culture 16:416-422
  16. Park JS, Chyun JH. 1993. Effects of low fat diet and saturated fat supplementation on the immune status of BALB/c mouse. Kor J Nutr 26:578-585
  17. Park KY, Lee SJ, Lee KI, Rhee SH. 2005. The antitumor effect in Sarcoma-180 tumor cell of mice of mice administered with Japanese apricot, garlic ginger doenjang. Kor J Food Cookery Sci 21:599-606
  18. Park MH, Kil KJ, Lee BW. 1998. Anti-cancer activity of Lentinus edoeds and Pleurotus ostreatus. Kor J Food Science & Technology 30:702-708
  19. Pisa P, Halapi E, Pisa EK, Gerdin E, Hising C, Buchi A. 1992. Selective expression of interleukin 10, interferon gamma and granulocyte-macrophage colony-stimulating factor in ovarian cancer biopsies. Proc Nual Acad Sci 89:7708-7712 https://doi.org/10.1073/pnas.89.16.7708
  20. Ryu HS, Jung YH, Kim HS. 2007. Effect of Hizikia fusiforme water extracts on mouse immune cell activation. Kor J Nutr 40:639-649
  21. Ryu HS. 2011. Effect of corn extracts on mouse spleen and cytokine production by peritoneal macrophage for four weeks. Korean J Food Nutr 24:65-70 https://doi.org/10.9799/ksfan.2011.24.1.065
  22. Ryu HS, Kim HS. 2005. Effects of job's tear extracts on mouse immune cell activation. J Korean Diet Assoc 11:44-50
  23. Ryu HS, Kim JH, Kim HS. 2006. Enhancing effect of Sorghum bicolor L. Moench (Sorghum, su-su) extracts on mouse spleen and macrophage cell activation. Kor J Food Nutr 19:176-182
  24. Ryu HS. 2010. Effects of water extract acorn on mouse immune cell activation ex vivo. Korean J Food Nutr 23:135-140
  25. Shan BE, Yoshida Y, Kuroda E, Yamashira U. 1999. Immunomodulating activity of seaweed extract on human lymphocytes in vitro. Int J Immunopharmacol 21:59-70 https://doi.org/10.1016/S0192-0561(98)00063-0
  26. Suh JS, Eun JS. 1998. Isolation of active components on immunocytes from Codonopsis lanceolatae. Korean J Community Nutrition 31:1076-1081
  27. Suh JS. 1996. Effect of Codonopsis lanceolata Radix water extract on immunocyte. Korean J Food & Nutr 9:379-384
  28. Sun JS. 1996. Effect of Codonopsis lanceolatae Radix water extract on immunocytes. Korean J Food Nutr 9:379-384
  29. Sypek JP, Chung CL, Mayer SH, Subramanyam JM, Goldman SJ, Sieburth DS, Wolf SF, Schaub RG. 1994. Ressolution of cutaneous leishmaniasis: interleukin 12 initiates a protective T helper type 1 immune response. J Exp Med 263:235-237

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Acknowledgement

Supported by : 상지대학교