Comparison of Bacterial Cultivation Results before and after Hand Washing from a College Student in Gangwon Province, Korea: Using Plain and Antibacterial Soap

일 지역 대학생의 세정제를 이용한 손 씻기 후 유해 미생물의 차이: 일반세정제와 항균세정제 차이를 중심으로

  • Joung, Hye Young (Department of Nursing, Songho College) ;
  • Choi, Yeonim (Department of Biomedical Laboratory Science, Songho College) ;
  • Hyun, Hye Jin (Department of Nursing, Kangwon National University) ;
  • Kim, Joo Hyun (Department of Nursing, Kangwon National University) ;
  • Yoon, Sung Ja (Department of Nursing, Songho College) ;
  • Lee, Gyusang (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
  • 정혜영 (송호대학교 간호과) ;
  • 최연임 (송호대학교 임상병리과) ;
  • 현혜진 (강원대학교 춘천캠퍼스 간호학과) ;
  • 김주현 (강원대학교 춘천캠퍼스 간호학과) ;
  • 윤성자 (송호대학교 간호과) ;
  • 이규상 (연세대학교 임상병리학과)
  • Received : 2014.04.01
  • Accepted : 2014.07.31
  • Published : 2014.08.31


Purpose: This study investigated the microorganisms in the hand before and after hand washing, using plain and antibacterial soap. The purpose of this study was to provide details, for educational purposes, of hand washing methods that should be used by college students for their hand hygiene. Methods: This study was arranged using a randomized control group pretest-posttest design. Data obtained through questionnaires were collected from college students in Gangwon Province. This study involved three groups under different conditions. The first and the second group washed their hands with plain and antibacterial soap, respectively. The third group members were educated about hand washing and they then washed their hands with plain soap. In addition, we collected samples to investigate the removal rate of Escherichia coli and Staphylococcus aureus, which can cause bacterial disease, by using sterile swabs from the hands in group 3. Results: The removal rates of bacteria in the first and the second group after hand washing with plain and antimicrobial soap were 62.7% and 76%, respectively. The third group, who were educated concerning proper hand washing, showed a figure of 72.8%. Conclusion: Repeated and more consistent education of college students concerning proper hand washing would be important in order to improve their knowledge, attitude, and performance with regard to hand washing.



  1. Britennica Online Korea.비누&DBase=Article_Up
  2. Han, M. J. (2003). The Effect of on Educational Hand washing Program on the Hnad washing Compliance. Unpublished master's thesis, Dankook, Seoul.
  3. Hand Washing Awareness Survey Findings Report and Recognition. (2005, October). Korea Centers for Disease Control (KCDC), from
  4. Hand Washing Practices and perception. (2009, December). Korea Centers for Disease Control (KCDC) from
  5. Jang, Y. J. (2007). A Study on the knowledge, attitude and practice of handwashing in middle school students. Unpublished master's thesis, Konyang University, Nonsan.
  6. Jeong, J. S., Choi, J. H., Lee, S. H., & Kim, Y. S. (2003). Hand hygiene effects measured by hand culture in intensive care unit. Korean Society of Biological Nursing Science, 5(2), 21-30.
  7. John, M. B., & Didier, P. (2002). Guidline for hand hygine in health-care settings, 51(RR-16): Atianta, Morbidity and Mortality Weekly Report (MMWR), CDC.
  8. Jones, R. D. (1999). Bacterial resistance and topical antimicrobial wash products. American Journal of infection control, 27, 351-363.
  9. Kang, Y. J. (2001). A Technical review: Handwashing, essential for safe food preparation. The Korean public health association, 27(4), 56-71.
  10. Kim, J. G., & Kim, J. S. (2009). A Study on the Hand-washing Awareness and Practices of Female University Students. Journal of Food Hygiene and Safety, 24(2), 128-135.
  11. Kim, J. H., Byeon, D. H., Kim, M. J., Sim, S. S., Choo, H. S., Chai, G. J., et al. (2011). Hand washing and prevention measures for new types of influenza. Korean Society of Biological Nursing Science, 13(1), 16-22.
  12. Kim, Y. J. (2013). A study on the Subjectivity about Nursing Student's Attitude after Hand Washing Education. The Journal of Digital Policy & Management, 11(12), 621-630.
  13. LeBlanc, D. M., Reece, E. M., Horton, J. B., & Janis, J. E. (2007). Increasing incidence of methicillin -resistant Staphylococcus aureus in hand infections: a 3-year county hospital experience. Plast Reconstr Surg, 119(3), 935-940.
  14. Lim, H. J. (1996). A Study on handwashing knowledge and attitudes of nurses. The Journal of Korean Nursing Administration Academic Society, 2(2), 5-16.
  15. Montville, R., Chen, Y., & Schaffner, D. W. (2002). Risk assessment of hand washing efficacy using literature and experimental data. International Journal of Food Microbiology I, 73, 305-313.
  16. Press Releases Online Survey of Adolescent Health Behaviors. (2012, November). Korea Centers for Disease Control (KCDC).
  17. Robinson, V. C., Bergfeld, W. F., Belsito, D. V., Hill, R. A., Klaassen, C. D., Marks, J. G. Jr, et al. (2010). International journal of toxicology, Author Robinson V. C., Year 2010, Vol 29 (4), 151-161.
  18. Trine, H., Roy, M. N., Kjersti, S., & Stig, H. (2009). Association between use of Hand Hygiene Products and Rates of Health Care-associated Infections in a Large University Hospital in Norway. Am J Infect Control, 37, 311-317.
  19. Widipedia. (2014).
  20. Widipedia. (2013).