Quality Characteristics of Citrus Fruit by Cyclic Low Pressure Drying and High Hydrostatic Pressure Extraction

초고압 처리에 의한 감귤의 추출률 및 특성변화

  • Park, Sung-Jin (Dept. of Tourism Food Service Cuisine, Hallym Polytechnic University Research Institute of Biomaterial, Hallym Polytechnic University) ;
  • Choi, Young-Bum (Agro Foodtech Holdings O'JEJU, Jeju Special Self-Governing Province) ;
  • Ko, Jung-Rim (Agro Foodtech Holdings O'JEJU, Jeju Special Self-Governing Province) ;
  • Rha, Young-Ah (Dept. of Food Technology and Services, Eulji Univrsity) ;
  • Lee, Hyeon-Yong (Dept. of Food Science and Technology, Seowon University)
  • 박성진 (한림성심대학교 관광외식조리과/한림성심대학교 생물소재연구소) ;
  • 최영범 (농업회사법인 (주)오'제주) ;
  • 고정림 (농업회사법인 (주)오'제주) ;
  • 나영아 (을지대학교 식품산업외식학과) ;
  • 이현용 (서원대학교 식품공학과)
  • Received : 2014.02.05
  • Accepted : 2014.06.10
  • Published : 2014.06.30

Abstract

We developed a method for improving the antioxidant activities of Citrus extracts through cyclic low pressure drying(CLPD) and a high hydrostatic pressure extraction (HPE) process. Citrus fruits were prepared for water extraction at $60^{\circ}C$ and 300 MPa for 5 min (high hydrostatic pressure extraction, HPE5) and 15 min (high hydrostatic pressure extraction, HPE15) after cyclic low pressure drying method. Extraction yields obtained by cyclic low pressure drying and high hydrostatic pressure extraction process were 20.41, 23.47, and 28.19%, respectively. Total polyphenol contents were increased by combined process. Generally, CLPD and HPE resulted in higher yields than the conventional extraction process. Further, HPE15 showed 48.21% DPPH radical scavenging activity (EDA, %) at $1,000{\mu}g/mL$. In general, antioxidant activities of Citrus increased by CLPD and HPE. Therfore, CLPD and HPE of Citrus resulted in higher antioxidant activity than conventional water extraction.

본 연구에서는 순환형 감압건조 및 초고압 추출공정을 이용하여 전통적인 기존 추출공정과 비교함으로써 복합 추출공정에 의한 감귤의 항산화 활성 증진을 확인하고자 연구를 수행하였다. 건조공정을 거친 후 초고압 처리 추출물의 수율이 20.41~28.19%로 높은 추출 수율을 나타내어 건조전 열수추출공정(17.21%)과 비교하여 약 1.6배의 높은 추출수율을 나타내었다. 순환형 감압건조와 초고압 공정을 병행하였을 시 총 폴리페놀과 플라보노이드 함량이 순환형 감압건조공정을 거치지 않은 것보다 다소 증가되는 것으로 보아 활성성분의 용출이 증진된 것으로 보인다. DPPH radical 소거 활성은 15분 초고압 처리한 추출물이 48.21%로 높은 활성을 나타내었다. 전처리 공정에 따른 감귤 시료의 주사전자현미경(SEM)을 통해 순환형 감압건조 후 초고압 추출이 감귤 내부 조직까지 영향을 주어 세포벽이 깨어지면서 조직 및 구조가 변화한 것으로 판단되며, 이를 통해 수율 및 활성 성분의 용출 증가가 이루어 진 것으로 사료된다. 따라서, 감귤의 건조 및 초고압 추출공정의 최적화를 통한 활성물질의 추출 극대화를 통해 추출수율을 향상시킬 것으로 판단된다.

Keywords

Acknowledgement

Supported by : 농림수산식품기술기획평가원

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