Association of DNA Methylation Levels with Tissue-specific Expression of Adipogenic and Lipogenic Genes in Longissimus dorsi Muscle of Korean Cattle

  • Baik, M. (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Vu, T.T.T. (Department of Molecular Biotechnology, Chonnam National University) ;
  • Piao, M.Y. (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kang, H.J. (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University)
  • Received : 2014.04.17
  • Accepted : 2014.06.24
  • Published : 2014.10.01


Epigenetic factors, such as DNA methylation status, may regulate adipogenesis and lipogenesis, thus affecting intramuscular fat (IMF) deposition in longissimus dorsi muscle (LM) of beef cattle. In Korean cattle steers, the LM consists mainly of muscle tissue. However, the LM tissue also contains IMF. We compared the gene expression levels between the IMF and muscle portions of the LM after tissue separation. Real-time polymerase chain reaction analysis showed that the mRNA levels of both adipogenic peroxisome proliferator-activated receptor gamma isoform 1 (PPARG1) and lipogenic fatty acid binding protein 4 (FABP4) were higher (p<0.01) in the IMF than in the muscle portion of the LM. We determined DNA methylation levels of regulatory regions of the PPARG1 and FABP4 genes by pyrosequencing of genomic DNA. DNA methylation levels of two of three CpG sites in the PPARG1 gene promoter region were lower (p<0.05) in the IMF than in the muscle portion of the LM. DNA methylation levels of all five CpG sites from the FABP4 gene promoter region were also lower (p<0.001) in the IMF than in the muscle portion. Thus, mRNA levels of both PPARG1 and FABP4 genes were inversely correlated with DNA methylation levels in regulatory regions of CpG sites of the corresponding gene. Our findings suggest that DNA methylation status regulates tissue-specific expression of adipogenic and lipogenic genes in the IMF and muscle portions of LM tissue in Korean cattle.


Adipogenesis;DNA Methylation;Intramuscular Fat;Korean Cattle


Supported by : Rural Development Administration


  1. Yanase, T., T. Yashiro, K. Takitani, S. Kato, S. Taniguchi, R. Takayanagi, and N. Nawata. 1997. Differential expression of PPAR$\gamma$1 and $\gamma$2 isoforms in human adipose tissue. Biochem. Biophys. Res. Commun. 233:320-324.
  2. Zhu, Y., K. Alvares, Q. Huang, M. S. Rao, and J. K. Reddy. 1993. Cloning of a new member of the peroxisome proliferator-activated receptor gene family from mouse liver. J. Biol. Chem. 268:26817-26820.
  3. Zych, J., M. A. Stimamiglio, A. C. Senegaglia, P. R. Brofman, B. Dallagiovanna, S. Goldenberg, and A. Correa. 2013. The epigenetic modifiers 5-aza-2'-deoxycytidine and trichostatin A influence adipocyte differentiation in human mesenchymal stem cells. Braz. J. Med. Biol. Res. 46:405-416.
  4. Jeong, J. Y., J. S. Kim, T. H. Nguyen, H. J. Lee, and M. Baik. 2013. Wnt/beta-catenin signaling and adipogenic genes are associated with intramuscular fat content in the longissimus dorsi muscle of Korean cattle. Anim. Genet. 44:627-635.
  5. Moisa, S. J., D. W. Shike, D. B. Faulkner, W. T. Meteer, D. Keisler, and J. J. Loor. 2014. Central role of the PPAR$\gamma$ gene network in coordinating beef cattle intramuscular adipogenesis in response to weaning age and nutrition. Gene Regul. Syst. Biol. 8:17-32.
  6. Musri, M. M., R. Gomis, and M. Parrizas. 2007. Chromatin and chromatin-modifying proteins in adipogenesis. Biochem. Cell Biol. 85:397-410.
  7. Noer, A., A. L. Sorensen, A. C. Boquest, and P. Collas. 2006. Stable CpG hypomethylation of adipogenic promoters in freshly isolated, cultured, and differentiated mesenchymal stem cells from adipose tissue. Mol. Biol. Cell 17:3543-3556.
  8. Sarjeant, K. and J. M. Stephens. 2012. Adipogenesis. Cold Spring Harbor Perspectives in Biology 4:a008417.
  9. Sorensen, A. L., S. Timoskainen, F. D. West, K. Vekterud, A. C. Boquest, L. Ahrlund-Richter, S. L. Stice, and P. Collas. 2010. Lineage-specific promoter DNA methylation patterns segregate adult progenitor cell types. Stem Cells Dev. 19:1257-1266.
  10. Sundvold, H., A. Brzozowska, and S. Lien. 1997. Characterisation of bovine peroxisome proliferator-activated receptors gamma 1 and gamma 2: genetic mapping and differential expression of the two isoforms. Biochem. Biophys. Res. Commun. 239:857-861.
  11. Vidal-Puig, A. J., R. V. Considine, M. Jimenez-Linan, A. Werman, W. J. Pories, J. F. Caro, and J. S. Flier. 1997. Peroxisome proliferator-activated receptor gene expression in human tissues. Effects of obesity, weight loss, and regulation by insulin and glucocorticoids. J. Clin. Invest. 99:2416-2422.
  12. Ahn, J., X. Li, Y. M. Choi, S. Shin, S. A. Oh, Y. Suh, T. H. Nguyen, M. Baik, S. Hwang, and K. Lee. 2014. Differential expressions of G0/G1 switch gene 2 and comparative gene identification-58 are associated with fat content in bovine muscle. Lipids 49:1-14.
  13. Bong, J. J., J. Y. Jeong, P. Rajasekar, Y. M. Cho, E. G. Kwon, H. C. Kim, B. H. Paek, and M. Baik. 2012. Differential expression of genes associated with lipid metabolism in longissimus dorsi of Korean bulls and steers. Meat Sci. 91:284-293.
  14. Deaton, A. M. and A. Bird. 2011. CpG islands and the regulation of transcription. Genes Dev. 25:1010-1022.
  15. Duarte, M. S., P. V. Paulino, A. K. Das, S. Wei, N. V. Serao, X. Fu, S. M. Harris, M. V. Dodson, and M. Du. 2013. Enhancement of adipogenesis and fibrogenesis in skeletal muscle of Wagyu compared with Angus cattle. J. Anim. Sci. 91:2938-2946.
  16. Elbrecht, A., Y. Chen, C. A. Cullinan, N. Hayes, M. D. Leibowitz, D. Moller, and J. Berger. 1996. Molecular cloning, expression and characterization of human peroxisome proliferator activated receptors g1 and g2. Biochem. Biophys. Res. Commun. 224:431-437.
  17. Fujiki, K., F. Kano, K. Shiota, and M. Murata. 2009. Expression of the peroxisome proliferator activated receptor gamma gene is repressed by DNA methylation in visceral adipose tissue of mouse models of diabetes. BMC Biol. 7:38.
  18. Jones, P. A. and D. Takai. 2001. The role of DNA methylation in mammalian epigenetics. Science 293:1068-1070.

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