Evaluation of the Efficacy of Fermented By-product of Mushroom, Pleurotus ostreatus, as a Fish Meal Replacer in Juvenile Amur Catfish, Silurus asotus: Effects on Growth, Serological Characteristics and Immune Responses

  • Katya, Kumar (Department of Marine Bio-materials and Aquaculture/Feeds and Foods Nutritional Research Center, Pukyong National University) ;
  • Yun, Yong-Hyun (Department of Marine Bio-materials and Aquaculture/Feeds and Foods Nutritional Research Center, Pukyong National University) ;
  • Park, Gunhyun (Department of Marine Bio-materials and Aquaculture/Feeds and Foods Nutritional Research Center, Pukyong National University) ;
  • Lee, Jeong-Yeol (Department of Aquaculture and Aquatic Science, Kunsan Nat'l University) ;
  • Yoo, Gwangyeol (The Province of Chungcheongnam-do Fisheries Research Institute) ;
  • Bai, Sungchul C. (Department of Marine Bio-materials and Aquaculture/Feeds and Foods Nutritional Research Center, Pukyong National University)
  • Received : 2014.01.16
  • Accepted : 2014.05.08
  • Published : 2014.10.01


The present experiment was conducted to evaluate the efficacy of dietary fermented by-product of mushroom, Pleurotus ostreatus, (FBPM) as a fish meal (FM) replacer in juvenile Amur catfish, Silurus asotus. A total number of 225 fish averaging $5.7{\pm}0.1g$ ($mean{\pm}standard$ deviation) were fed one of the five experimental diets formulated to replace FM with FBPM at 0%, 5%, 10%, 20%, and 30% ($FBPM_0$, $FBPM_5$, $FBPM_{10}$, $FBPM_{20}$, and $FBPM_{30}$, respectively). At the end of eight weeks of the experiment, average weight gain (WG) of fish fed $FBPM_0$ or $FBPM_5$ were significantly higher than those of fish fed $FBPM_{20}$ or $FBPM_{30}$ diets (p<0.05). However, there was no significant differences in WG among the fish fed $FBPM_0$, $FBPM_5$ or $FBPM_{10}$, and between fish fed $FBPM_{10}$ or $FBPM_{20}$, and also between those fed $FBPM_{20}$ or $FBPM_{30}$ diets. Lysozyme activity of fish fed $FBPM_0$ or $FBPM_5$ were significantly higher than those of fish fed $FBPM_{10}$, $FBPM_{20}$ or $FBPM_{30}$ diets (p<0.05). The chemiluminescent response of fish fed $FBPM_5$ was significantly higher than those of fish fed $FBPM_0$, $FBPM_{20}$ or $FBPM_{30}$ diets (p<0.05). Broken line regression analysis of WG suggested that the maximal dietary inclusion level for FBPM as a FM replacer could be 6.3% without any adverse effects on whole body composition and on serological characteristics. Therefore, these results may indicate that the maximal dietary inclusion level of FBPM as a FM replacer could be 6.3% in juvenile Amur catfish.


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