DOI QR코드

DOI QR Code

Pig Spermatozoa Defect in Acrosome Formation Caused Poor Motion Parameters and Fertilization Failure through Artificial Insemination and In vitro Fertilization

  • Lee, Won Young ;
  • Lee, Ran ;
  • Kim, Hee Chan ;
  • Lee, Kyung Hoon ;
  • Cui, Xiang Shun ;
  • Kim, Nam Hyung ;
  • Kim, Sang Hyun ;
  • Lee, Il Joo ;
  • Uhm, Sang Jun ;
  • Yoon, Min Jung ;
  • Song, Hyuk
  • Received : 2014.03.20
  • Accepted : 2014.05.27
  • Published : 2014.10.01

Abstract

The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology. However, these primary parameters are often not reliable for discriminating between normal and abnormal, non-fertilizable spermatozoa. The present study was designed to compare the motion characteristics, fertilization ability using in vitro fertilization (IVF), and acrosome formation of the semen from boars having low (boar number 2012) and normal (boar number 2004 and 2023) breeding performances. The ultimate goal was to identify additional simple and easy criteria for the selection of normal sperm. There was no significant difference between boar 2004 and boar 2023 sperm total motility in computer assisted sperm analysis. However, boar number 2012 semen presented a significantly reduced population of rapid moving spermatozoa and an increased population of slow moving spermatozoa compared to boar numbers 2004 and 2023. Analysis of detailed motion characteristics revealed that sperm from boar number 2012 had significantly reduced motility in progressiveness, average path velocity, straight-line velocity (VSL), curvilinear velocity (VCL), straightness, and linearity. The assessment of the fertilizing ability by IVF also showed that sperm from boar number 2012 showed a fertility rate of 3.4%, whereas sperm from boar number 2023 had a fertility rate of 75.45%. Interestingly, most of the sperm nuclei were found on the peripheral area of the oocytes, suggesting that the sperm from boar number 2012 lacked penetration ability into the oocyte zonapellucida. The acrosome formation analysis using Pisum sativum agglutinin staining demonstrated that the sperm from boar number 2012 had a defect in acrosome formation. Consequently, primary parameters for selecting semen before AI such as motility are not sufficient to select normal and fertilizable spermatozoa. In conclusion, the present study suggests that the acrosome staining and detailed motion characteristics such as progressiveness, VCL, and VSL should be included in determining semen quality together with primary parameters for successful AI and high breeding performance in the swine industry.

Keywords

Boar;Sperm;Motion Parameters;In vitro Fertilization;Acrosome Defect

References

  1. Aitken, R. J., L. Kerr, V. Bolton, and T. Hargreave. 1990. Analysis of sperm function in globozoospermia: implications for the mechanism of sperm-zona interaction. Fertil. Steril. 54:701-707.
  2. Amann, R. P. 1989. Can the fertility potential of a seminal sample be predicted accurately? J. Androl. 10:89-98.
  3. Bavister, B. D., M. L. Leibfried, and G. Lieberman. 1983. Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol. Reprod. 28:235-247. https://doi.org/10.1095/biolreprod28.1.235
  4. Blasco, L. 1984. Clinical tests of sperm fertilizing ability. Fertil. Steril. 41:177-192.
  5. Bostofte, E., J. Serup, and H. Rebbe. 1984. Interrelations among the characteristics of human semen, and a new system for classification of male infertility. Fertil. Steril. 41:95-102.
  6. Chenoweth P. J. 2005. Genetic sperm defects. Theriogenology 64:457-468. https://doi.org/10.1016/j.theriogenology.2005.05.005
  7. Correa, J. R. and P. M. Zavos. 1994. The hypoosmotic swelling test: Its employment as an assay to evaluate the functional integrity of the frozen-thawed bovine sperm membrane. Theriogenology 42:351-360. https://doi.org/10.1016/0093-691X(94)90280-1
  8. Flowers, W. L. 1997. Management of boars for efficient semen production. J. Reprod. Fertil. Suppl. 52:67-78.
  9. Foldesy, R. G., M. M. Vanderhoof, and D. W. Hahn. 1986. Lack of correlation between fertility and sperm numbers in male rats treated with histrelin, a potent LHRH agonist. J. Androl. 7:140-146.
  10. Gil, M. A., M. Ruiz, J. M. Vazquez, J. Roca, B. N. Day, and E. A. Martinez. 2004. Effect of short periods of sperm-oocyte coincubation during in vitro fertilization on embryo development in pigs. Theriogenology 62:544-552. https://doi.org/10.1016/j.theriogenology.2003.11.001
  11. Glass, R. H. and R. J. Ericsson. 1979. Spontaneous cure of male infertility. Fertil. Steril. 31:305-308.
  12. Abeydeera, L. R. and B. N. Day. 1997. In vitro penetration of pig oocytes in a modified Tris-buffered medium: Effect of BSA, caffeine and calcium. Theriogenology 48:537-544. https://doi.org/10.1016/S0093-691X(97)00270-7
  13. Saacke, R. G. 1990. What is abnormal? And is abnormal dependent upon the animal. In: Proceeding of the NAAB 13th Technical Conference on AI and Reprod. Milwaukee, WI, USA pp. 67-73.
  14. Santos, N. R., N. Krekeler, A. Schramme-Jossen, and D. H. Volkmann. 2006. The knobbed acrosome defect in four closely related dogs. Theriogenology 66:1626-1628. https://doi.org/10.1016/j.theriogenology.2006.02.006
  15. Soderquist, L. 1998. Reduced fertility after artificial insemination in a ram with a high incidence of knobbed acrosomes. Vet. Rec. 143:227-228. https://doi.org/10.1136/vr.143.8.227
  16. Toyama, Y. and Y. Itoh. 1993. Ultrastructural features and pathogenesis of knobbed spermatozoa in a boar. Am. J. Vet. Res. 54:743-749.
  17. World Health Organization. 1992. WHO Labboratory manual for the Examination of human semen and semen-cervical mucus interaction. Cambridge University Press, New York, NY, USA.
  18. Yoshioka K., C. Suzuki, A. Tanaka, I. M. Anas, and S. Iwamura. 2002. Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol. Reprod. 66:112-119. https://doi.org/10.1095/biolreprod66.1.112
  19. Hoing, L. M., P. Devroey, and A. C. Van Steirteghem. 1986. Treatment of infertility because of oligoasthenoteratospermia by transcervical intrauterine insemination of motile spermatozoa. Fertil. Steril. 45:388-391.
  20. Ha, W. T., W. Y. Lee, R. Lee, J. H. Kim, N. H. Kim, J. H. Kim, I. J. Lee, and H. Song. 2013. Effect of naturally derived substances on motion parameters of in vitro non-freezing preserved pig sperm. Reprod. Dev. Biol. 37:9-16. https://doi.org/10.12749/RDB.2013.37.1.9
  21. Hurtgen, J. P. and L. A. Johnson. 1982. Fertility of stallions with abnormalities of the sperm acrosome. J. Reprod. Fertil. Suppl. 32:15-20.
  22. Ivanova, M. and M. Mollova. 1993. Zona-penetration in vitro test for evaluating boar sperm fertility. Theriogenology 40:397-410. https://doi.org/10.1016/0093-691X(93)90277-C
  23. Lavara, R., E. Moce, F. Lavara, M. P. Viudes de Castro, and J. S. Vicente. 2005. Do parameters of seminal quality correlate with the results of on-farm inseminations in rabbits? Theriogenology 64:1130-1141. https://doi.org/10.1016/j.theriogenology.2005.01.009
  24. Moretti, E., G. Collodel, G. Scapigliati, I. Cosci, B. Sartini, and B. Baccetti. 2005. 'Round head' sperm defect. Ultrastructural and meiotic segregation study. J. Submicrosc. Cytol. Pathol. 37:297-303.
  25. Nicander, L. and A. Bane. 1966. Fine structure of the sperm head in some mammals, with particular reference to the acrosome and the subacrosomal substance. Z. Zellforsch. Mikrosk. Anat. 72:496-515. https://doi.org/10.1007/BF00319255
  26. Quintero-Moreno, A., T. Rigau, and J. E. Rodriguez-Gil. 2007. Multivariate cluster analysis regression procedures as tools to identify motile sperm subpopulations in rabbit semen and to predict semen fertility and litter size. Reprod. Domest. Anim. 42:312-319. https://doi.org/10.1111/j.1439-0531.2006.00785.x
  27. Robinson, J. A. and M. M. Buhr. 2005. Impact of genetic selection on management of boar replacement. Theriogenology 63:668-678. https://doi.org/10.1016/j.theriogenology.2004.09.040
  28. Saacke, R. G. 1983. Semen quality in relation to semen preservation. J. Dairy Sci. 66:2635-2644. https://doi.org/10.3168/jds.S0022-0302(83)82137-7

Cited by

  1. Improvement of Boar Semen Quality by Sperm Selection Using Magnetic Nano-particles vol.26, pp.8, 2016, https://doi.org/10.5352/JLS.2016.26.8.943
  2. Novel agents for sperm purification, sorting, and imaging vol.84, pp.9, 2017, https://doi.org/10.1002/mrd.22831
  3. Nanotechnology-based approach for safer enrichment of semen with best spermatozoa vol.10, pp.1, 2019, https://doi.org/10.1186/s40104-018-0307-4

Acknowledgement

Supported by : Rural Development Administration