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The Role of CTGF in Osteosarcoma Progression

골육종에서 CTGF의 발현과 발암기전에서의 역할

  • Han, Ilkyu (Department of Orthopaedic Surgery, Seoul National University Hospital) ;
  • Lee, Mi Ra (Department of Orthopaedic Surgery, Seoul National University Hospital) ;
  • Kim, Han-Soo (Department of Orthopaedic Surgery, Seoul National University Hospital)
  • 한일규 (서울대학교병원 정형외과) ;
  • 이미라 (서울대학교병원 정형외과) ;
  • 김한수 (서울대학교병원 정형외과)
  • Received : 2014.03.31
  • Accepted : 2014.06.04
  • Published : 2014.06.30

Abstract

Purpose: To examine the expression of Connective Tissue Growth Factor (CTGF) in osteosarcoma and to evaluate its role in osteosarcoma invasion and proliferation. Materials and Methods: The mRNA expression of CTGF from 23 patient-derived osteosarcoma cell lines was examined, and the role of CTGF in cell invasion and proliferation was examined using siRNA transfection. Results: The over-expression of CTGF mRNA was observed in 17 cell lines (74%). CTGF-specific siRNA transfection into SaOS-2 and MG63 cell lines resulted in efficient knockdown of CTGF expression on Western blot analysis. siRNA transfected cells showed decreased migration on Matrigel invasion assay and decreased cell proliferation on WST-1 assay. Conclusion: These results indicated that the CTGF expression may play an important role in osteosarcoma progression, and may be a therapeutic target of osteosarcoma.

Acknowledgement

Supported by : 서울대학교병원

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