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The Interaction between HCV-Infected huh7.5 Cells and HCV-Specific T Cells

C형 간염 바이러스 감염 간암 세포주와 T 림프구의 상호작용에 대한 연구

  • Kang, Hyojeung (Department of Pharmacy, College of Pharmacy, Kyungpook National University) ;
  • Cho, Hyosun (Department of Pharmacy, College of Pharmacy, Duksung Women's University)
  • 강효정 (경북대학교 약학대학 약학과) ;
  • 조효선 (덕성여자대학교 약학대학 약학과)
  • Received : 2014.03.28
  • Accepted : 2014.05.30
  • Published : 2014.06.30

Abstract

Recently, Hepatitis C virus (HCV) replication system has been established using human hepatoma cells (huh cell) and a variety of HCV clones. In this study, we established an infectious HCV replication system using huh7.5 cells and J6/JFH1 clone (genotype 2a). In addition, we investigated the antigen presentation capability of HCV-infected huh7.5 cells to HCV-specific T cells. Interestingly, HCV-infected huh7.5 cells were not capable of activating HCV-specific T cells. However, huh7.5 cells stimulated by exogenous HCV peptide were able to activate HCV-specific T cells, which was shown to produce TNF-${\alpha}$ and IFN-${\gamma}$. We further examined if HCV infection has an inhibitory effect on the expression of MHC class I molecule of huh7.5 cells. We found that HCV infection did not change the expression level of MHC class I molecule on huh7.5 cells.

Acknowledgement

Supported by : 덕성여자대학교

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