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Influence of Curcumin on HOTAIR-Mediated Migration of Human Renal Cell Carcinoma Cells

  • Pei, Chang-Song (The First Clinical Medical College, Nanjing University of Chinese Medicine) ;
  • Wu, Hong-Yan (Department of Pharmacy, Yancheng Health Vocational and Technical College) ;
  • Fan, Fan-Tian (School of Pharmaceutical Science, Nanjing University of Chinese Medicine) ;
  • Wu, Yi (Department of Urology, The Second Affiliated Hospital of Xuzhou Medical School) ;
  • Shen, Cun-Si (School of Pharmaceutical Science, Nanjing University of Chinese Medicine) ;
  • Pan, Li-Qun (The First Clinical Medical College, Nanjing University of Chinese Medicine)
  • Published : 2014.05.30

Abstract

Background: This study investigated the influence of curcumin on HOX transcript antisense RNA (HOTAIR)-mediated migration of cultured renal cell carcinoma (RCC) cells. Materials and Methods: Five RCC cell lines (769-P, 769-P-vector, 769-P-HOTAIR, 786-0, and Kert-3 ) were maintained in vitro. The expression of HOTAIR mRNA was determined by quantitative real-time PCR and cell migration was measured by transwell migration assay. The effects of different concentrations of curcumin (0 to $80{\mu}mol/L$) on cell proliferation was determined by the CCK-8 assay and influence of non-toxic levels (0 to $10{\mu}M$) on the migration of RCC cells was also determined. Results: Comparison of the 5 cell lines indicated a correlation between HOTAIR mRNA expression and cell migration. In particular, the migration of 769-P-HOTAIR cells was significantly higher than that of 769-P-vector cells. Curcumin at $2.5-10{\mu}M$ had no evident toxicity against RCC cells, but inhibited cell migration in a concentration-dependent manner. Conclusions: HOTAIR expression is correlated with the migration of RCC cells, and HOTAIR may be involved in the curcumin-induced inhibition of RCC metastasis.

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