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DOI QR Code

Inactivated Sendai Virus Strain Tianjin Induces Apoptosis in Human Breast Cancer MDA-MB-231 Cells

  • Chen, Jun (Department of Microbiology, Basic Medical College, Tianjin Medical University) ;
  • Han, Han (Department of Microbiology, Basic Medical College, Tianjin Medical University) ;
  • Chen, Min (Department of Nursing, The 44th Hospital of PLA) ;
  • Xu, Xiao-Zhu (The Second People Hospital of Guizhou Province) ;
  • Wang, Bin (Department of Anesthesiology, Tianjin Research Institute of Anesthesiology, Tianjin Medical University General Hospital) ;
  • Shi, Li-Ying (Department of Microbiology, Basic Medical College, Tianjin Medical University)
  • Published : 2014.06.30

Abstract

Sendai virus strain Tianjin is a novel genotype. Here, we investigate the antitumor and proapoptotic effects of ultraviolet-inactivated Sendai virus strain Tianjin (UV-Tianjin) on human breast cancer MDA-MB-231 cells in vitro, as well as the involvement of the apoptotic pathway in the mechanism of UV-Tianjin-induced antitumor effects. MTT assays showed that treatment with UV-Tianjin dose-dependently inhibited the proliferation of MDA-MB-231 cells but not normal MCF 10A breast epithelium cells. Hoechst staining and flow cytometric analysis revealed that UV-Tianjin induced apoptosis of MDA-MB-231 cells in a dose-dependent manner. Moreover, UV-Tianjin treatment resulted in reduction in the mitochondria membrane potential (MMP) and release of cytochrome complex (cyt c) via regulation of Bax and Bcl-2, as well as activation of caspase-9, caspase-3, Fas, FasL and caspase-8 in MDA-MB-231 cells. In summary, our study suggests that UV-Tianjin exhibits anticancer activity in human breast cancer MDA-MB-231 cells through inducing apoptosis, which may involve both the endogenous mitochondrial and exogenous death receptor pathways.

Acknowledgement

Supported by : National Natural Science Foundation of China

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