Analysis on the Components and Safety Evaluation of Abeliophyllum distichum Nakai Leaves and Stems

미선나무 잎과 줄기의 성분 분석 및 안전성 평가

  • Received : 2014.05.13
  • Accepted : 2014.06.19
  • Published : 2014.06.30


Objectives: This study was carried out in order to analyze the composition of the leaves and stems of Abeliophyllum distichum Nakai, with the aim of obtaining basic data for utilizing the plant as a food ingredient, as well as for processing. Methods: Leaves and stems from Abeliophyllum distichum Nakai were harvested at Cheongcheon-myeon, Geosan-gun, Chungcheongbuk-do, and were subsequently freeze-dried and ground to a fine powder for chemical component analysis and safety evaluation. Results: The moisture contents of Abeliophyllum distichum Nakai leaves and stems were respectively 65.07% and 40.97%, and the crude ash contents were 1.32% and 0.91%. In addition, the crude protein contents were 11.97% and 3.77%, and the crude fat contents were 2.52% and 0.36%, respectively. The fructose and glucose contents were 32.13 mg/g and 56.17 mg/g for leaves, and 11.38 mg/g and 10.59 mg/g for stems. The major fatty acids of the leaves were palmitic acid (31.79%) and stearic acid (14.79%), and those for stems were linolenic acid (32.78%) and palmitic acid (26.75%). The ascorbic acid contents of leaves and stems were 1.32 mg/g and 0.30 mg/g respectively. The calcium content was found to be the highest among the minerals tested, both in the leaves and stems, with the levels being 166.17 mg/100 g for leaves and 592.34 mg/100 g for stems. The content of organic acid was greater in leaves than in stems, with that of malic acid accounting for more than 75% of total organic acids for both samples. The total phenolic compounds and flavonoid contents of Abeliophyllum distichum Nakai were 50.64 mg/g and 13.53 mg/g in leaves and 96.47 mg/g and 18.53 mg/g in stems, respectively. No changes were shown in the number of micronucleated polychromatic erythrocytes (MNPCE) among 2,000 polychromatic erythrocytes compared to the negative control. Abeliophyllum distichum Nakai was administered orally to rats in order to investigate acute toxicity. The $LD_{50}$ values in rats were above 2,000 mg/kg. Conclusion: These results indicate that the leaves and stems of Abeliophyllum distichum Nakai can be used as natural ingredients in the development of nutritional and functional materials.


Abeliophyllum distichum Nakai;Nutritional components;Functional components;Safety evaluation


  1. Pyo YH, Lee TC, Logendra L, Rogen RT. Antioxidant activity and phenolic compounds of Swiss chard extracts. Food Chem. 2004; 85(1): 19-26.
  2. Elzaawely AA, Xuan TD, Koyama H, Tawata S. Antioxidant activity and contents of essential oil and phenolic compounds in flowers and seeds of A. zerumbet (Pers.). Food Chem. 2007; 104(4): 1648-1653.
  3. Hertog M, Feskens E, Kromhout D. Antioxidant flavonols and coronary heart disease risk. Lancet. 1997; 349(9053): 699-705
  4. Lee YS. Analysis of components in the different parts of Ailanthus altissima. Kor J Food Preserv. 2008; 159(2): 261-268.
  5. Tian S, Wang Y, Nakamura k. Correlation of polyphenolics content to antioxidant activity of Forsythia Suspensa leaves. In: Shinshu University International Symposium. Matsumoto: Shinshu University Press; 2010. p.79-84.
  6. Kim EJ, Choi JY, Yu MR, Kim MY, Lee SH, Lee BH. Total Polyphenols, Total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Kor J Food Sci Technol. 2012; 44(3): 337-342.
  7. Hong SG, Chung SG, Hyun SH. The micronucleus test of the diglyceride preparation with conjugated linoleic acid by using mice. J Kor Soc Food Sci Nutr. 2008; 37: 853-857
  8. Kim SJ, Cho HW, Rim KT, Maeng SH, Kim HY. Bacterial reverse mutation test of 1,2,4-trimethylbenzene. J Environ Toxcol. 2006; 21: 317-322.
  9. Jeong CH, Hur JY, Shim KH. Chemical components, antioxidative and antimicrobial activities of chestnut (Castanea crenata) leaves. Kor J Food Preserv. 2002; 9(1): 234-239.
  10. Lee YS, Joo EY, Kim NW. Analysis on the components in stem of the Lespedeza bicolor. J Kor Soc Food Sci Nutr. 2005; 34(8): 1264-1250.
  11. Lee OH, Lee HB, Lee JS, Son JY, Rhee SK, Kim HD, et al. Chemical properties of olive and bay leaves. J Kor Soc Food Sci Nutr. 2005; 34(4): 503-508.
  12. Hur CH, Jeong Ch, Shim KH. Chemical components of Humulus Japonicus leaves and stalks. J Agric Life Sci. 2003; 37(1): 1-7.
  13. Shin KK, Yang CB, Park H. Studies on lipid and fatty acid composition of Korean Perilla leaves (Penilla frutescens var. japonica HARA). Kor J Food Sci Technol. 1992; 24(6): 610-615.
  14. Byers T, Perry G. Dietary carotenes, vitamin C, and vitamin E as protective antioxidants in human cancers. Annu Rev Nutr. 1992; 12: 139-159.
  15. Lee YJ, Ahn MS, Hong KH. A study on the content of general compounds, amino acid, vitamins, catechins, alkaloids in green, oolong and black tea. J Food Hygiene Safety. 1998; 13(4): 377-382.
  16. Lee JS, Han GC, Han GP, Nobuyuki K. The antioxidant activity and total polyphenol content of Cudrania tricuspidata. J East Asian Soc Dietary Life. 2007; 17(5): 696-702.
  17. Park SK, Cho YS, Park JR, Chun SS, Moon JS. Non-volatile organic acids, mineral, fatty acids and fiber compositions in dolsan leaf mustard(Brassica juncea). J Kor Soc Food Nutr. 1993; 22(1): 53-57.
  18. Wang Q, Kuang H, Su Yang, Sun Y, Feng J, Guo R, et al. Naturally derived anti-inflammatory compounds from Chinese medicinal plants. J Ethnopharmacol. 2013; 146(1): 9-39.
  19. Yoo YK, Kim KS. Effects of plant growth regulators and cutting conditions on rooting of softwood and semihardwood cutting in white forsythia. J Kor Soc Hort Sci. 1997; 38(3): 263-271.
  20. Yoo YK, Kim KS. Germination inhibitors in the seed coat of white forsythia (Abeliophyllium destichum Nakai. J Kor Soc Hort Sci. 2001; 42(1): 6-10.
  21. Park JH. Antioxidant activities and inhibitory effect on oxidative DNA damage of extracts from Abeliophylli distichi folium. Kor J Herbology. 2011; 26(4): 95-99.
  22. AOAC. Official methods of analysis, 16th ed. Washington: Association of official analytical chemists Press; 2000. p.17-24.
  23. Dewanto V, Wu X, Adom KK, Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidative activity. J Agric Food Chem. 2002; 50: 3010-3014.
  24. Nieva MMI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol. 2000; 71(1-2): 109-114.
  25. Kim J, Jeong CH, Bae YI, Shim KH. Chemical components of Zanthoxylum schinifolium and Zanthoxylum piperitum leaves. Kor J Postharvest Sci Technol. 2000; 7(2): 189-194.
  26. Jeong CH, Shim KH. Chemical components in leaf and Fruit stalk of Hovenia dulcis Thunb. Kor J Postharvest Sci Technol. 1999; 6(4): 469-471.
  27. Kim JB, Cui CB, Lee DS, Ham SS. Ham SS. Studies on the composition and antioxidative effect of leaves from Korean Rosa davurica Pall. Kor J Food Preserv. 2004; 1(1): 106-110.
  28. Yoon KY, Hong JY, Shin SR. Analysis on the components of the Elaeagnus multiflora Thunb. leaves. Kor J Food Preserv. 2007; 14(6): 639-644.
  29. Lee TB. Edemic plants and their distribution in Korea. Bulletin of the Arboretum Seoul National University. 1983; 4: 71-113.
  30. Kim EJ, Choi JW, Yu MR, Kim MY, Lee SG, Lee BH. Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Kor J Food Sci Technol. 2012; 44(3): 337-342.
  31. Lee TB. Conservation of threatened plants in Korea. Bulletin of the Arboretum Seoul National University. 1980; 3: 190-196.
  32. Kim YS. Morphological and ecological characteris tics of Abeliophyllum destichem Nakai populations in Korea. J Resource Development. 1998; 17(1): 67-81.
  33. Jin YH, You JH, Cho HW, Lee CH. Flower color morphological characteristics of Abeliophyllum spp. Kor Flower Res Soc. 2005; 13(2): 83-89.
  34. Lee WT, Kil BS. The investigation on natural growth region of Abeliophyllum distichum Nakai. Kor J Plant Tax. 1991; 21(1): 1-8.
  35. Lim DO, Hwang IC, Choi HW, Kim YS. Characteristics and management proposal of Abeliophyllum distichum subpopulation in the Byeonsanbando national park. Kor J Environ Ecol. 2009; 23(2): 116-126.
  36. Park CM, Park SH, Oh HK, Soh MS. Characteristics and management Method of Abeliophyllum distichum new habitats in the Byeonsanbando. J Agric Life Sci. 2010; 41(2): 58-66.
  37. Lee HY, Kim TG, Oh CH. Recently Augmented natural habitat of Abeliophyllum distichum Nakai in Yeoju-si, Gyunggi-do, Korea. Kor J Environ Ecol. 2014; 28(1): 62-70.

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