Effect on Pancreatic Beta Cells and Nerve Cells by Low LET X-ray

Low LET X-ray가 췌장 ${\beta}$ 세포와 신경세포에 미치는 효과

  • Park, Kwang-Hun (Department of Nuclear Medicine, Kyungbuk National University Hospital) ;
  • Kim, Kgu-Hwan (Department of Radiological Technology, Daegu health College)
  • Received : 2013.12.19
  • Accepted : 2014.03.13
  • Published : 2014.03.31

Abstract

Cultured pancreatic beta cells and nerve cells, it is given normal condition of 10% FBS (fetal bovine serum), 11.1 mM glucose and hyperglycemia codition of 1% FBS, 30 mM glucose. For low LET X-ray irradiated with 0.5 Gy/hr dose-rate(total dose: 0.5 to 5 Gy). Survival rates were measured by MTT assay. When non irradiated, differentiated in the pancreatic beta cells experiment is hyperglycemia conditions survival rate compared to normal conditions survival rate seemed a small reduction. However increasing the total dose of X-ray, the survival rate of normal conditions decreased slightly compared to the survival rate of hyperglycemia conditions, the synergistic effect was drastically reduced. When non irradiated, undifferentiated in the nerve cells experiment is hyperglycemia conditions survival rate compared to normal conditions survival rate seemed a large reduction. As the cumulative dose of X-ray normal conditions and hyperglycemia were all relatively rapid cell death. But the rate of decreased survivals by almost parallel to the reduction proceed and it didn't show synergistic effect.

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