노간주나무(Juniperus rigida Sieb) 추출물의 항산화 활성 및 α-amylase와 α-glucosidase에 대한 저해활성

Antioxidant activity and inhibition activity against α-amylase and α-glucosidase of Juniperus rigida Sieb extracts

  • 김정환 (호서대학교 한방화장품과학과) ;
  • 이수연 (호서대학교 한방화장품과학과) ;
  • 박정미 (호서대학교 한방화장품과학과) ;
  • 박주훈 (호서대학교 한방화장품과학과) ;
  • 권오준 (대경지역사업평가원) ;
  • 이진영 (호서대학교 한방화장품과학과)
  • Kim, Jeung-Hoan (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Lee, Soo-Yeon (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Park, Jung-Mi (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Park, Joo-Hoon (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Kwon, O-Jun (Daegyeong Institute for Regional Program Evaluation) ;
  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University)
  • 투고 : 2013.11.08
  • 심사 : 2014.03.27
  • 발행 : 2014.06.30


본 연구에서는 노간주나무 추출물의 항산화 및 ${\alpha}$-amylase와 ${\alpha}$-glucosidase을 조사하여 항산화소재로 이용하고자 실험하였다. 노간주나무 추출물의 항산화효과를 살펴보기 위하여 총 폴리페놀 함량을 측정한 결과 열수추출물은 71.3mg/g, 에탄올추출물은 116.0 mg/g 함량을 포함하고 있었으며, 플라보노이드 함량은 열수추출물은 17.7 mg/g, 에탄올추출물은 76.4 mg/g 함량을 포함하고 있었다. ABTS 라디칼 소거능을 측정한 결과 $500{\mu}g/mL$ 농도에서 열수추출물은 76.4%, 에탄올 추출물은 79.3% 라디칼 소거능을 나타내었다. FRAP 값을 측정한 결과 열수추출물에서 1.83 mM를 에탄올추출물에서 1.77 mM로 열수추출물에서 더 높은 효과를 나타내었다. ACE 활성억제효과는 $500{\mu}g/mL$ 농도에서 열수추출물 75.39%, 에탄올 추출물 71.25%로 저해율을 나타내었다. 노간주나무 추출물의 antioxidant protection factor를 측정한 결과 열수추출물의 경우는 1.5 PF, 에탄올추출물의 경우는 2.1 PF를 나타내었다. TBARS 저해율은 $500{\mu}g/mL$ 농도에서 열수추출물과 에탄올추출물 각각 55.78%와 71.48%을 나타내었다. ${\alpha}$-glucosidase와 ${\alpha}$-amylase 저해효과를 측정한 결과 $500{\mu}g/mL$ 농도에서 각각 70%와 90% 이상의 저해효과를 나타내어 항산화 및 항당뇨 예방물질 소재로의 활용이 가능할 것으로 판단된다.


연구 과제 주관 기관 : 보건복지부


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