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Antioxidant activity and inhibition activity against α-amylase and α-glucosidase of Juniperus rigida Sieb extracts

노간주나무(Juniperus rigida Sieb) 추출물의 항산화 활성 및 α-amylase와 α-glucosidase에 대한 저해활성

  • Kim, Jeung-Hoan (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Lee, Soo-Yeon (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Park, Jung-Mi (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Park, Joo-Hoon (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Kwon, O-Jun (Daegyeong Institute for Regional Program Evaluation) ;
  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University)
  • 김정환 (호서대학교 한방화장품과학과) ;
  • 이수연 (호서대학교 한방화장품과학과) ;
  • 박정미 (호서대학교 한방화장품과학과) ;
  • 박주훈 (호서대학교 한방화장품과학과) ;
  • 권오준 (대경지역사업평가원) ;
  • 이진영 (호서대학교 한방화장품과학과)
  • Received : 2013.11.08
  • Accepted : 2014.03.27
  • Published : 2014.06.30

Abstract

In this study, the antioxidant effect of water and ethanol extracts from Juniperus rigida Sieb were investigated. The activities of each of the extracts were measured based on their total phenolic and flavonoid contents and using antioxidant test such as of 2,2-azinobis (3-ethyl benzothiazoline-6-sulfonic acid (ABTs) radical scavenging activities, ferric reducing antioxidant power (FRAP), angiotensin I converting enzyme (ACE) inhibition activity, antioxidant protection fator (PF), thiobarbituric acid reactive substances (TBARs) content, and ${\alpha}$-glucosidase and ${\alpha}$-amylase inhibition activity assay. The result of the examination to measure the polyphenol content by investigating the antioxidativity of the J. rigida Sieb. extract showed 71.3 mg/g polyphenol content in the water extract, and 116.0 mg/g in the ethanol extract and a 17.7 mg/g flavonoid content in the water extract and in 76.4 mg/g in the ethanol extract. The ABTS radical cation decolorization showed 76.4% and 79.3% scavenging activities of the $500{\mu}g/mL$ water extract and ethanol extract, respectively. The FRAP showed 1.83 mM efficacy in the water extract and a lower 1.77 mM in ethanol extract. Both the water extract and the ethanol extract showed reduced ACE activities of 75.39% and 71.25% at $500{\mu}g/mL$, respectively. The antioxidant protection factor of the water and 70% ethanol extracts of J. rigida Sieb were 1.5 PF and 2.1 PF, respectively. In the TBARS inhibitory activity, the extracts showed 55.78% and 71.48% antioxidant activities at the $500{\mu}g/mL$ concentration. The results of the measurrement of the ${\alpha}$-amylase inhibitory activity indicated more than 90% of activity inhibition in the $500{\mu}g/mL$ concentration of the ethanol extract. For the ${\alpha}$-glucosidase inhibitory activity, the ethanol extract showed 70% activity inhibition at the $500{\mu}g/mL$ concentration.

Acknowledgement

Supported by : 보건복지부

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