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The quantitative analysis by the image processing of sperm changes according to the radiation irradiation of white rat testicle

흰쥐 정소의 방사선 조사에 따른 정자변화의 영상처리에 의한 정량분석

  • Na, Soo-Kyung (Dept. of Radiological Science, Gimcheon University) ;
  • Kim, Sung-In (Dept. of Biomedical Laboratory Science, Gimcheon University) ;
  • Lee, On-Seok (Dept. of Radiological Science, Gimcheon University)
  • 나수경 (김천대학교 간호보건대학 방사선학과) ;
  • 김성인 (김천대학교 간호보건대학 임상병리학과) ;
  • 이언석 (김천대학교 간호보건대학 방사선학과)
  • Received : 2014.03.20
  • Accepted : 2014.06.20
  • Published : 2014.06.28

Abstract

This study aims to get more accurate and objective result by quantifying the result for the sperm changes through the quantitative analysis by the image processing based on the image obtained microscopically for the testicle cell and sperm change appeared with the passage of time when the radiation is irradiated to the white rat testicle. This study has targeted the white rat of 8 weeks lifespan, the X-ray of 6 MV with 1 time of 2 Gy has been irradiated to the whole body. The testicles of 5 rats at each test group immediately after irradiation, after 2 hours of irradiation, 4 hours, 8 hours and 24 hours has been respectively extracted targeting all 30 white rats of normal control group not irradiated by the radiation and the test group. The state of testicle cell and sperm has been observed in the normal control group and the test group by implementing Periodic acid Schiff dyeing after extraction. 24 hours after irradiation, a gradual decrease in sperm count and testicular cells qualitatively and quantitatively that were identified as significant.

Keywords

Radiation Irradiation;Sperm;Histochemical Image;Image Processing;Quantitative Analysis;White Rat

References

  1. Hasegawa, M. G. Wilson, L. D. Russell, and M. L. Meistrich, Radiation-induced cell death in the mouse testes: relationship to apoptosis. Radiat, Res, 147, 457-467, 1997. https://doi.org/10.2307/3579503
  2. Y. Kamiguchi, H. Tateno, Radiation-and chemical-induced structural chromosome aberrations in human spermatozoa. Mutat, Res, 504, 183-191. 2002. https://doi.org/10.1016/S0027-5107(02)00091-X
  3. M. L. Meistrich, Efects of Chemotherapy and Radiotherapy on Spermatogenesis, Eur Urol, 23(1), 136-141, 1993. https://doi.org/10.1159/000474582
  4. J. S. Jang, J. S. Kim, S. H. Kim, Evaluation of Radiation-induced Apoptosis in Seminiferous Tubule of ICR Mouse after Gamma Irradiation, Journal of Life Science, 19(6), 799-803, 2009. https://doi.org/10.5352/JLS.2009.19.6.799
  5. G. Liu, P. Gong, H. Zhao, Z. Wang, S. Gong, L. Cai. Effect of low-level radiation on the death of male germ cells. Toksikol, 165(4), 379-389. 2006.
  6. O. Lee, S. H. Jeong, W. U. Shin, G. Lee, C. Oh, & S. W. Son, Influence of surface charge of gold nanorods on skin penetration. Skin Research and Technology, 19(1), e390-e396. 2013. https://doi.org/10.1111/j.1600-0846.2012.00656.x
  7. T. S. Oh, O. Lee, J. E. Kim, S. W. Son, & C. H. Oh, Quantitative method for measuring therapeutic efficacy of the 308 nm excimer laser for vitiligo. Skin Research and Technology, 18(3), 347-355. 2012. https://doi.org/10.1111/j.1600-0846.2011.00575.x