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Photodynamic effect of Photofrin and Radachlorin against Staphylococcus aureus and Staphylococcus epidermidis

황색포도알균과 표피포도알균에 대한 포토프린과 라다클로린의 광역학 효과

  • Seo, Choong-Won (Dept. of Biomedical Laboratory Science, Seonam University) ;
  • Ryu, Jae-Gi (Dept. of Biomedical Laboratory Science, Gimcheon University) ;
  • Kwon, Pil-Seung (Dept. of Biomedical Laboratory Science, Wonkwang Health Science University)
  • 서충원 (서남대학교 임상병리학과) ;
  • 류재기 (김천대학교 임상병리학과) ;
  • 권필승 (원광보건대학교 임상병리과)
  • Received : 2014.03.20
  • Accepted : 2014.06.20
  • Published : 2014.06.28

Abstract

The aim of this study was to evaluate the photodynamic therapy effects against staphylococci using Photofrin and Radachlorin with Light emitting diode(LED). Experimental methods, The bacterial suspensions containing Staphylococcus aureus and Staphylococcus epidermidis at $1{\times}10^5$ were prepared and diluted to different concentrations of photosensitizer, Photofrin or Radachlorin, on 1.25, 2. 5,5 and $10{\mu}g/ml$. The bacterial suspensions were exposed to 630 and 670 nm LED light at the energy density of 14.4 and $19.8J/cm^2$, respectively. The CFU results of S. aureus and S. epidermidis were showed 33 and 50 colony forming at $5{\mu}g/ml$ of Photofrin, respectively and both of them perfectely were dead at $5{\mu}g/ml$ of Radachlorin. The fluorescent intensity by flow cytometry was showed the increase in the dead cells than the normal cells. In the TEM photograph, the damage of bacterial membrane and the distortion of cell morphology were observed. These results suggest that photodynamic therapy combine with Photofrin and Radachlorin can be applied a new modality for antibacterial therapy.

Keywords

Photodynamic therapy(PDT);Photofrin;Radachlorin;Staphylococcus aureus;Staphylococcus epidermidis

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