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Genotoxicity of Environment-friendly Organic Materials of Plant Origin in the Micronucleus Test Using Chinese Hamster Lung Cells

Chinese Hamster Lung Cell의 소핵시험을 이용한 식물추출물 유기농업자재의 유전독성평가

  • Cho, Hyeon-Jo (Chemical Safety Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Park, Kyung-Hun (Chemical Safety Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Jeong, Mi Hye (Agro-Material Safety Evaluation Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Park, Soo Jin (Korea Bio-Safety Institute Co., ltd.) ;
  • Oh, Jin-Ah (Chemical Safety Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Won-Il (Microbial Safety Team, National Institute of Agricultural Science, Rural Development Administration) ;
  • Cho, Namjun (Chemical Safety Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Ryu, Jae-Gee (Microbial Safety Team, National Institute of Agricultural Science, Rural Development Administration) ;
  • Paik, Min-Kyoung (Chemical Safety Division, National Institute of Agricultural Science, Rural Development Administration)
  • 조현조 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 박경훈 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 정미혜 (농촌진흥청 국립농업과학원 농자재평가과) ;
  • 박수진 (한국생물안전성연구소) ;
  • 오진아 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 김원일 (농촌진흥청 국립농업과학원 유해생물팀) ;
  • 조남준 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 류재기 (농촌진흥청 국립농업과학원 유해생물팀) ;
  • 백민경 (농촌진흥청 국립농업과학원 화학물질안전과)
  • Received : 2014.05.08
  • Accepted : 2014.06.10
  • Published : 2014.06.30

Abstract

BACKGROUND: Azadirachta Indica extract(AIE) and Sophorae radix extract(SRE) are widely used as environment-friendly organic materials of plant origin in South Korea. METHODS AND RESULTS: In this study, the in vitro micronucleus(vitMN) tests of two samples of AIE and SRE were conducted to evaluate their genotoxicity using the Chinese hamster lung(CHL) cell. This study was composed of two parts; cytochalasin B(cyto B) test and non-cyto B test. Mitomycin C and colchicine were used as positive controls. As a result, the incidence of micronucleus(MN) in all AIE and SRE treated groups increased in dose-dependent manner, but were less than 2.2% in 1,000 binucleated cells. In addition, there were no significant increases of MN incidence in all AIE and SRE treated groups, compared with the negative control group. CONCLUSION: Therefore, we suggest that AIE samples and SRE samples used in this study may have no genotoxicity in the in vitro micronucleus test using the CHL cells. In our previous study, we reported that AIE and SRE did not cause genotoxicity in Ames test. According to the genotoxicity battery system, we concluded that AIE and SRE used in this study have no genotoxic effects to humans.

Keywords

Azadirachta indica extract;Genotoxicity;In vitro micronucleus test;Sophorae radix extract

Acknowledgement

Grant : Research Program for Agricultural Science & Technology Development

Supported by : National Academy of Agricultural Science

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