Study of Kidney Toxicity of Azadirachta Indica Extract for Oral Administration in Rats

님추출물의 경구투여에 따른 랫드의 신장독성 연구

  • Yoon, Hyunjoo (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Choe, Miseon (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Cho, Hyeon-Jo (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Han, Beom Seok (Hoseo Toxicological Research Center, Hoseo University) ;
  • Park, Kyung-Hun (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Oh, Jin-Ah (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Cho, Namjun (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Paik, Min-Kyoung (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration)
  • 윤현주 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 최미선 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 조현조 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 한범석 (호서대 안전성평가센터) ;
  • 박경훈 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 오진아 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 조남준 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 백민경 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과)
  • Received : 2013.12.05
  • Accepted : 2014.03.26
  • Published : 2014.06.30


BACKGROUND: Azadirachta indica has been widely used as environment-friendly organic materials because of its insecticidal properties. This study was carried out to investigate the acute toxicity and the subacute toxicity of Azadirachta indica extract(AIE) in rats. METHODS AND RESULTS: For the oral acute toxicity test, Sprague-Dawley rats were gavaged with 2.0 g/Kg bw of AIE. The $LD_{50}$ value was greater than 2.0 g/Kg bw for both male and female rats. For the subacute toxicity study, rats were treated with AIE at doses of 0.5, 1.0, 2.0 mg/Kg bw once a day for 4 weeks(n=10 animals per each group). There were no significant changes in body weight, food intake and water consumption observed during the experimental duration. In addition, no difference of relative kidney weight was observed among all treated groups. Serum creatinine level in the AIE 2.0 g/Kg group increased significantly compared with that of control group in male rats, but serum blood urea nitrogen was significantly decreased in a dose-dependent manner (p<0.05). Significant increase of serum cholesterol levels were observed in all AIE groups, compared with the control group, in the female rats (p<0.05). However, histopathological examination of the kidney did not reveal any significant lesions in all groups. CONCLUSION: On the basis of results, it could be concluded that oral administration AIE didn't cause any toxic response in kidney, except the increased serum cholesterol.


Azadirachta indica extract;Kidney;Subacute toxicity


Grant : Research Program for Agricultural Science & Technology Development

Supported by : National Academy of Agricultural Science


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