- Volume 15 Issue 5
DOI QR Code
in vitro Assessment of Antineoplastic Effects of Deuterium Depleted Water
- Soleyman-Jahi, Saeed (Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences) ;
- Zendehdel, Kazem (Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences) ;
- Akbarzadeh, Kambiz (Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences) ;
- Haddadi, Mahnaz (Valie-Asr Reproductive Health Research Center) ;
- Amanpour, Saeid (Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences) ;
- Muhammadnejad, Samad (Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences)
- Published : 2014.03.01
Background: In vitro, in vivo and clinical studies have demonstrated anti-cancer effects of deuterium depleted water (DDW). The nature of this agents action, cytotoxic or cytostatic, remains to be elucidated. We here aimed to address the point by examining effects on different cell lines. Materials and Methods: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) -based cytotoxicity analysis was conducted for human breast, stomach, colon, prostate cancer and glioblastoma multiforme cell lines as well as human dermal fibroblasts. The cell lines were treated with decreasing deuterium concentrations of DDW alone, paclitaxel alone and both. One way analysis of variance (ANOVA) was used for statistical analysis. Results: Treatment with different deuterium concentrations of DDW alone did not impose any significant inhibitory effects on growth of cell lines. Paclitaxel significantly decreased the survival fractions of all cell lines. DDW augmented paclitaxel inhibitory effects on breast, prostate, stomach cancer and glioblastoma cell lines, with influence being more pronounced in breast and prostate cases. Conclusions: DDW per se does not appear to have inhibitory effects on the assessed tumor cell lines as well as normal fibroblasts. As an adjuvant, however, DDW augmented inhibitory effects of paclitaxel and thus it could be considered as an adjuvant to conventional anticancer agents in future trials.
- Bild W, Nastasa V, Haulica I (2004). In vivo and in vitro research on the biological effects of deuterium-depleted water: 1. Influence of deuterium-depleted water on cultured cell growth. Rom J Physiol, 41, 53-67.
- Cong FS, Zhang YR, Sheng HC, et al (2010). Deuteriumdepleted water inhibits human lung carcinoma cell growth by apoptosis. Exp Ther Med, 1, 277-83.
- Eisenhauer EA, Therasse P, Bogaerts J, et al (2009). New response evaluation criteria in solid tumours: revised RECIST guideline (version 1.1). Eur J Cancer, 45, 228-47. https://doi.org/10.1016/j.ejca.2008.10.026
- Gyongyi Z, Somlyai G (2000). Deuterium depletion can decrease the expression of C-myc Ha-ras and p53 gene in carcinogentreated mice. In Vivo, 14, 437-9.
- Gyongyi Z, Budan F, Szabo I, et al (2013). Deuterium depleted water effects on survival of lung cancer patients and expression of Kras, Bcl2, and Myc genes in mouse lung. Nutr Cancer, 65, 240-6. https://doi.org/10.1080/01635581.2013.756533
- Jordan MA, Wilson L (2004). Microtubules as a target for anticancer drugs. Nat Rev Cancer, 4, 253-65. https://doi.org/10.1038/nrc1317
- Katz JJ, Crespi HL (1971). Isotope effects in biological systems. In 'Iso-tope effects in chemical reactions',Eds C. J. Collins and N. S. Bowman. Van Nostrand Rein-hold, New York, 286-363.
- Korn EL, Arbuck SG, Pluda JM, et al (2001). Clinical trial designs for cytostatic agents: are new approaches needed? J Clin Oncol, 19, 265-72.
- Kovacs A, Guller I, Krempels K, et al (2011). Deuterium depletion may delay the progression of prostate cancer. J Cancer Therapy, 2, 548-56. https://doi.org/10.4236/jct.2011.24075
- Krempels K, Somlyai I, Somlyai G (2008). A retrospective evaluation of the effects of deuterium depleted water consumption on 4 patients with brain metastases from lung cancer. Integr Cancer Ther, 7, 172-81. https://doi.org/10.1177/1534735408322851
- Nagy LI, Fabian G, Puskas LG (2012). Effects of deuterium depleted water alone and in combination of known chemotherapeutic agents on different tumor cells. 2nd international congress on deuterium depletion. Budapest, Hungary, ECB.
- Plumb JA, Milroy R, Kaye SB (1989). Effects of the pH dependence of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide-formazan absorption on chemosensitivity determined by a novel tetrazolium-based assay. Cancer Res, 49, 4435-40.
- Roumyantsev SA, Osipova EY, Kozlitina OV, et al (2012). The effect of DDW on the kinetics of tumor clone in in vitro experiments. 2nd international congress on deuterium depletion. Budapest, Hungary, ECB.
- Somlyai G, Jancso G, Jakli G, et al (1993). Naturally occurring deuterium is essential for the normal growth rate of cells. FEBS Lett, 317, 1-4. https://doi.org/10.1016/0014-5793(93)81479-J
- Somlyai G, Laskay G, Berkenyi T, Jakli G, Jancso G (1997). Naturally occuring deuterium may have a central role in cell signalling. In 'Synthesis and application of isotopically labelled compounds', Eds J. R. Heys and D. G. Mellilo. John Wiley & Sons Ltd, New Jersy, 137-41.
- Somlyai G, Laskay G, Berkenyi T, et al (1998). The biological effects of deuterium-depleted water, a possible new tool in cancer therapy Z Onkol. J Oncol, 4, 91-4.
- Somlyai G (2004). Deuterium depletion and its impact on life expectancy of patients with stage iv breast tumor. J Complement Alternat Med, 8, 30-5.
- Somlyai G, Jancso G, Jakli G, et al (2010). Naturally occurring deuterium is essential for the normal growth rate of cells. 1st internation symposium on deuterium depletion. Budapest, Hungary.
- Somlyai G, Molnar M, Laskay G, et al (2010). Biological significance of naturally occurring deuterium: the antitumor effect of deuterium depletion. Orv Hetil, 151, 1455-60. https://doi.org/10.1556/OH.2010.28865
- Wang H, Zhu B, Liu C, Fang W, Yang H (2012). Deuteriumdepleted water selectively inhibits nasopharyngeal carcinoma cell proliferation in vitro. Nan Fang Yi Ke Da Xue Xue Bao, 32, 1394-9.
- Prognostic Significance of Preoperative and Postoperative Plasma Levels of Ghrelin in Gastric Cancer: 3-Year Survival Study vol.8, pp.1, 2017, https://doi.org/10.1038/ctg.2016.64
- The promising role of monoclonal antibodies for gastric cancer treatment vol.11, pp.4, 2019, https://doi.org/10.2217/imt-2018-0093