Ferutinin, an Apoptosis Inducing Terpenoid from Ferula ovina

  • Matin, Maryam Moghaddam (Department of Biology, Faculty of Science, Ferdowsi University of Mashhad) ;
  • Nakhaeizadeh, Hossein (Department of Biology, Faculty of Science, Ferdowsi University of Mashhad) ;
  • Bahrami, Ahamd Reza (Department of Biology, Faculty of Science, Ferdowsi University of Mashhad) ;
  • Iranshahi, Mehrdad (Biotechnology Research Center and School of Pharmacy, Mashhad University of Medical Sciences) ;
  • Arghiani, Nahid (Department of Biology, Faculty of Science, Ferdowsi University of Mashhad) ;
  • Rassouli, Fatemeh Behnam (Department of Biology, Faculty of Science, Ferdowsi University of Mashhad)
  • 발행 : 2014.03.01


A current hurdle in cancer management is the intrinsic or acquired resistance of cancer cells to chemical agents that restricts the efficacy of therapeutic strategies. Accordingly, there is an increasing desire to discover new natural compounds with selective toxicity to combat malignancies. In present study, the cytotoxic and apoptosis-inducing activities of ferutinin, a terpenoid derivative from Ferula ovina, were investigated on human breast (MCF7) and bladder (TCC) cancer cells as well as normal fibroblasts (HFF3).The toxicity and DNA damage inducing effects of ferutinin were studied by MTT and comet assays, DAPI and PI staining and DNA laddering. The $IC_{50}$ values of ferutinin were identified and compared with routine prescribed drugs, doxorubicin and vincristine, by MTT test. Alkaline comet assay and DAPI staining revealed DNA damage due to ferutinin, which was significantly (p<0.001) higher in MCF7 and TCC than HFF3 cells. Apoptosis induction was evidenced by PI staining and DNA laddering. Our results suggest that ferutinin could be considered as an effective anticancer agent for future in vivo and clinical experiments.


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