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Ethanolic Extract from Derris scandens Benth Mediates Radiosensitzation via Two Distinct Modes of Cell Death in Human Colon Cancer HT-29 Cells

  • Hematulin, Arunee (Radiobiology Research Unit, Department of Radiation Technology, Faculty of Allied Health Sciences) ;
  • Ingkaninan, Kornkanok (Department of Pharmaceutical Chemistry and Pharmacognosy, Naresuan University) ;
  • Limpeanchob, Nanteetip (Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Naresuan University) ;
  • Sagan, Daniel (Radiobiology Research Unit, Department of Radiation Technology, Faculty of Allied Health Sciences)
  • Published : 2014.02.28

Abstract

Enhancing of radioresponsiveness of tumors by using radiosensitizers is a promising approach to increase the efficacy of radiation therapy. Recently, the ethanolic extract of the medicinal plant, Derris scandens Benth has been identified as a potent radiosensitizer of human colon cancer HT29 cells. However, cell death mechanisms underlying radiosensitization activity of D scandens extract have not been identified. Here, we show that treatment of HT-29 cells with D scandens extract in combination with gamma irradiation synergistically sensitizes HT-29 cells to cell lethality by apoptosis and mitotic catastrophe. Furthermore, the extract was found to decrease Erk1/2 activation. These findings suggest that D scandens extract mediates radiosensitization via at least two distinct modes of cell death and silences pro-survival signaling in HT-29 cells.

Keywords

Radiosensitizer;Derris scandens benth;radiotherapy;apotosis mitotic catastrophe

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