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Demethoxycurcumin from Curcuma longa Rhizome Suppresses iNOS Induction in an in vitro Inflamed Human Intestinal Mucosa Model

  • Somchit, Mayura (School of Allied Health Sciences and Public Health) ;
  • Changtam, Chatchawan (Faculty of Science and Technology, Huachiew Chalermprakiet University) ;
  • Kimseng, Rungruedi (Center for Scientific and Technological Equipments, Walailak University) ;
  • Utaipan, Tanyarath (School of Allied Health Sciences and Public Health) ;
  • Lertcanawanichakul, Monthon (School of Allied Health Sciences and Public Health) ;
  • Suksamrarn, Apichart (Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University) ;
  • Chunglok, Warangkana (School of Allied Health Sciences and Public Health)
  • Published : 2014.02.28

Abstract

Background: It is known that inducible nitric oxide synthase (iNOS)/nitric oxide (NO) plays an integral role during intestinal inflammation, an important factor for colon cancer development. Natural compounds from Curcuma longa L. (Zingiberaceae) have long been a potential source of bioactive materials with various beneficial biological functions. Among them, a major active curcuminoid, demethoxycurcumin (DMC) has been shown to possess anti-inflammatory properties in lipopolysaccharide (LPS)-activated macrophages or microglia cells. However, the role of DMC on iNOS expression and NO production in an in vitro inflamed human intestinal mucosa model has not yet been elucidated. This study concerned inhibitory effects on iNOS expression and NO production of DMC in inflamed human intestinal Caco-2 cells. An in vitro model was generated and inhibitory effects on NO production of DMC at 65 ${\mu}M$ for 24-96 h were assessed by monitoring nitrite levels. Expression of iNOS mRNA and protein was also investigated. DMC significantly decreased NO secretion by 35-41% in our inflamed cell model. Decrease in NO production by DMC was concomitant with down-regulation of iNOS at mRNA and protein levels compared to proinflammatory cytokine cocktail and LPS-treated controls. Mechanism of action of DMC may be partly due to its potent inhibition of the iNOS pathway. Our findings suggest that DMC may have potential as a therapeutic agent against inflammation-related diseases, especially in the gut.

Keywords

Curcuminoids;inflamed human intestinal Caco-2 cells;iNOS/NO;in vitro model

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