DOI QR코드

DOI QR Code

High Expression of MICA in Human Kidney Cancer Tissue and Renal Cell Carcinoma Lines

  • Jia, Hong-Ying (Department of Evidence-Based Medicine, The Second Hospital, Shandong University) ;
  • Liu, Jun-Li (Clinical Molecular Biology Laboratory, The Second Hospital, Shandong University) ;
  • Zhou, Cheng-Jun (Department of Pathology, The Second Hospital, Shandong University) ;
  • Kong, Feng (Central Research Laboratory, The Second Hospital, Shandong University) ;
  • Yuan, Ming-Zhen (Department of Urology, The Second Hospital, Shandong University) ;
  • Sun, Wen-Dong (Department of Urology, The Second Hospital, Shandong University) ;
  • Wang, Jue (Central Research Laboratory, The Second Hospital, Shandong University) ;
  • Liu, Ling (Clinical Molecular Biology Laboratory, The Second Hospital, Shandong University) ;
  • Zhao, Jing-Jie (Clinical Molecular Biology Laboratory, The Second Hospital, Shandong University) ;
  • Luan, Yun (Central Research Laboratory, The Second Hospital, Shandong University)
  • Published : 2014.02.28

Abstract

The overall incidence and mortality of renal cell carcinoma (RCC), the most common kidney cancer, are steadily increasing for reasons that are not fully explained. Our aim was to explore the expression of membrane MHC class I chain-related gene A (mMICA) in human RCC cell lines and tissue specimens, and to determine expression of soluble MICA (sMICA) in serum of patients with renal cell carcinoma, we used flow cytometry (FCM) and immunohistochemistry as well as an enzyme linked immunosorbent assay (ELISA). The results showed that percentage of mMICA expression was significantly increased in human kidney cancer tissues and RCC cell lines (786-O and Ketr-3) than that in healthy adults and human embryonic kidney 293 (HEK293) cell line individuality (P<0.05). sMICA content in healthy adults was negative, but in renal cancer patients was significantly elevated (P<0.05). Our research showed that high expression of MICA in human kidney cancer, this results show that MICA might serve as potential tumor-associated antigen (TAA) in RCC.

Keywords

Renal cell carcinoma;mMICA;sMICA

References

  1. Raffaghello L, Prigione I, Airoldi I, et al (2004). Down regulation and/or release of NKG2D ligands as immune evasion strategy of human neuroblastoma. Neoplasia, 6, 558-68. https://doi.org/10.1593/neo.04316
  2. Herberman RB, Nunn ME, Lavrin DH (1975). Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic acid allogeneic tumors. I. Distribution of reactivity and specificity. Int J Cancer, 16, 216-29. https://doi.org/10.1002/ijc.2910160204
  3. Mrozek E, Anderson P, Caligiuri MA (1996). Role of interleukin-15 in the development of human CD56+ natural killer cells from CD34+ hematopoietic progenitor cells. Blood, 87, 2632-40.
  4. Pardoll DM (2001). Immunology. Stress, NK receptors, and immune surveillance. Science, 294, 534-6. https://doi.org/10.1126/science.1066284
  5. Sconocchia G, Spagnoli GC, Del Principe D, et al (2009). Defective infiltration of natural killer cells in MICA/Bpositive renal cell carcinoma involves beta (2)-integrinmediated interaction. Neoplasia, 11, 662-71. https://doi.org/10.1593/neo.09296
  6. Weissinger D, Tagscherer KE, Macher-Goppinger S, et al (2013). The soluble Decoy receptor 3 is regulated by a PI3Kdependent mechanism and promotes migration and invasion in renal cell carcinoma. Mol Cancer, 12, 120. https://doi.org/10.1186/1476-4598-12-120
  7. Wu JD, Higgins LM, Steinle A, et al (2004). Prevalent expression of the immunostimulatory MHC class I chain-related molecule is counteracted by shedding in prostate cancer. J Clin Invest, 114, 560-8. https://doi.org/10.1172/JCI200422206
  8. Yang F, Shao Y, Yang F, et al (2013). Valproic acid upregulates NKG2D ligand expression and enhances susceptibility of human renal carcinoma cells to NK cell-mediated cytotoxicity. Arch Med Sci, 9, 323-31.
  9. Ye YB, Zhou ZF, Chen Q, et al (2008). The roles of soluble MICA in immune escape of breast tumor. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 24, 904-8 (Article in Chinese).
  10. Diefenbach A, Jensen ER, Jamieson AM, Raulet DH (2001). Rae1 and H60 ligands of the NKG2D receptor stimulate tumour immunity. Nature, 413, 165-71. https://doi.org/10.1038/35093109
  11. Bauer S, Groh V, Wu J, et al (1999). Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science, 285, 727-9. https://doi.org/10.1126/science.285.5428.727
  12. Busche A, Goldmann T, Naumann U, et al (2006). Natural killer cell-mediated rejection of experimental human lung cancer by genetic overexpression of major histocompatibility complex class I chain-related gene A. Hum Gene Ther, 17, 135-46. https://doi.org/10.1089/hum.2006.17.135
  13. Cohen HT and McGovern FJ (2005). Renal-cell carcinoma. N Engl J Med, 353, 2477-90. https://doi.org/10.1056/NEJMra043172
  14. Diefenbach A and Raulet DH (2002). The innate immune response to tumors and its role in the induction of T-cell immunity. Immunol Rev, 188, 9-21. https://doi.org/10.1034/j.1600-065X.2002.18802.x
  15. Doubrovina ES, Doubrovin MM, Vider E (2003). Evasion from NK cell immunity by MHC class I chain-related molecules expressing colon adenocarcinoma. J Immunol, 171, 6891-9. https://doi.org/10.4049/jimmunol.171.12.6891

Cited by

  1. Epidemiology and Trends in Incidence of Kidney Cancer in Iran vol.16, pp.14, 2015, https://doi.org/10.7314/APJCP.2015.16.14.5859
  2. Regulation Roles of MICA and NKG2D in Human Renal Cancer Cells vol.16, pp.9, 2015, https://doi.org/10.7314/APJCP.2015.16.9.3901
  3. The clinical and biological significance of MICA in clear cell renal cell carcinoma patients vol.37, pp.2, 2016, https://doi.org/10.1007/s13277-015-4041-7
  4. Identification of genes associated with renal cell carcinoma using gene expression profiling analysis vol.12, pp.1, 2016, https://doi.org/10.3892/ol.2016.4573